1 (School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China) 2 (Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China) 3 (Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning 530004, China)
Abstract: To investigate the effects of ultrasonic mutagenesis on the content and activity of crude polysaccharides in mycelium and sporophore of Hericium erinaceus. The strains of Hericium erinaceus were subjected to ultrasonic mutagenesis at different times. After each mutagenized strain was initial screened by plate culture and then rescreened by liquid fermentation culture, a mutant strain of Hericium erinaceus with the highest biomass and the highest crude polysaccharide content was obtained finally. The original strain and the H-2 strain were then subjected to bag cultivation to obtain sporophores, and the crude polysaccharide content of the sporophores were compared. Finally, the antibacterial activities of the crude polysaccharides in the original strain and H-2 strain sporophores were compared. The crude polysaccharide contents of mycelium and sporophores of mutant strain H-2 were increased by 1.2% and 2.3%, respectively, and the crude polysaccharide of H-2 sporophore had significant antibacterial effect against Staphylococcus aureus. The content and antibacterial activity of crude polysaccharides in mycelium and sporophore can be improved by ultrasonic mutagenesis of Hericium erinaceus strains at an appropriate time.
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