The functional effects of extraction with acetone form Acaudina molpadioideagonad in vitro
XU Yangli1, SU Laijin1*, YANG Huicheng2, LI Ruixue3
1 (Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China) 2 (Zhejiang Marine Development Research Institute, Zhoushan 316021, China) 3 (Zhoushan Kejia Technical Service Co., Ltd, Zhoushan 316021, China)
Abstract: The present study aims to investigate the functional activities of extraction with acetone form Acaudina molpadioidea gonad (Am-GE) in vitro. The effects of Am-GE on cell proliferation were detected by MTT method in HepG2 and Caco-2 cells. Am-GE-influenced cell apoptosis and cycle were measured by flow cytometry. The inflammatory gene mRNA relative expression levels were determined by quantitative real time PCR in the macrophage of mice. Results showed that Am-GE could significantly inhibited cell proliferation in HepG2 and Caco-2 cells, promoted cell apoptosis and the ratio of G2 stage of HepG2 cells, mitigated the tumorigenic ability in Caco-2 cells. Meanwhile, Am-GE significantly inhibited the inflammatory genes relative expression at transcriptional level, namely TNF-α, IL-1β, IL-6 and iNOS. These indicated that Am-GE exhibited better anti-tumor and anti-inflammatory effects.
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2020, Vol. 46 
