Abstract: This study aims to obtain the best preparation technology of nano-chitosan particles and study its antimicrobial activity. Nano-chitosan particles were prepared by the ionic crosslinking method using chitosan and sodium tripolyphosphate as raw materials. Based on the single factor experiment, the preparation process of nano-chitosan particles was optimized by response surface analysis, and its antimicrobial activity was studied. The optimum preparation process is as follows: Chitosan concentration was 0.76 mg/mL, sodium triphosphate concentration was 0.77 mg/mL, the volume ratio of chitosan to sodium triphosphate was 4.11∶1, and the pH value of chitosan solution was 5.0. The theoretical predictions of particle size and Zeta potential of nano-chitosan particles were 122.3 nm and +23.43 mV, respectively. Confirmatory experiments showed that the size of nano-chitosan particles was 119.2 nm and the zeta potential was +22.8 mV, and the deviations from the theoretical values were 2.53% and 2.69% respectively, and nano-chitosan particles were distributed uniformly. Nano-chitosan particles concentration 0.6mg/mL could inhibit E. coli and S. aureus significantly. In conclusion, the preparation process is simple without adding organic reagents; the nano-chitosan particles prepared with this method have a small particle size, uniform distribution and remarkable antimicrobial effect. The experimental results provide a theoretical reference for the preparation and application of nano-chitosan particles.
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2020, Vol. 46 
