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食品与发酵工业  2020, Vol. 46 Issue (2): 266-271    DOI: 10.13995/j.cnki.11-1802/ts.021898
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
双波长可见吸收光谱法测定糕点及碳酸饮料中的苯甲酸
江虹*, 庞向东, 曾晓莎
(长江师范学院 化学化工学院,重庆,408100)
Determination of benzoic acid in pastries and carbonated beverages by dual- wavelength visible absorption spectrometry
JIANG Hong*, PANG Xiangdong, ZENG Xiaosha
(College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China)
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摘要 该文建立快速测定糕点及碳酸饮料中苯甲酸的双波长可见吸收光谱法。研究表明,在pH 8.54 Tris-盐酸介质中,苯甲酸与乙基紫反应生成二元离子缔合物。最大负吸收波长位于497 nm,次大负吸收波长位于628 nm,苯甲酸的质量浓度在0.03~1.2 mg/L 时与吸光强度呈良好的线性关系,服从朗伯-比尔定律。表观摩尔吸光系数(κ)分别为 5.04×104 L/(mol·cm)(497 nm)和3.70×104L/(mol·cm)(628 nm),检出限为0.026 mg/L(497 nm)和0.028 mg/L(628 nm),碳酸饮料的定量限为4.33 mg/L(497 nm) 和4.66 mg/L(628 nm),糕点的定量限为14.4 mg/kg(497 nm)和15.4 mg/kg(628 nm)。用双波长法测定时,线性范围为0.02~1.2 mg/L,表观摩尔吸光系数为8.74×104L/(mol·cm),检出限为0.014 mg/L,碳酸饮料和糕点的定量限分别为2.33和7.33 mg/kg,加标回收率为97.1%~103%,相对标准偏差RSD(n=5)为2.0%~2.8%。该法简便、快速、灵敏,适于碳酸饮料及糕点中苯甲酸的测定。
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江虹
庞向东
曾晓莎
关键词:  碳酸饮料  糕点  苯甲酸  乙基紫  双波长  吸收光谱法    
Abstract: A double wavelength visible absorption spectrometry method for the rapid determination of benzoic acid in pastries and carbonated beverages was developed. In a Tris- hydrochloric acid medium of pH 8.54, benzoic acid reacted with ethyl violet to form a binary ionic association complex. The maximum negative absorption wavelength was located at 497 nm, and the second largest negative absorption wavelength was at 628 nm. The mass concentration of benzoic acid in the range of 0.03 to 1.2 mg/L has a good linear relationship with the light absorption intensity, and the Lambert-beer's law was obeyed. The apparent molar absorptivity (κ) were 5.04×104 L/ (mol·cm) (497 nm) and 3.70×104 L/ (mol·cm) (628 nm), respectively. The detection limit was 0.026 mg/L (497 nm) and 0.028 mg/L (628 nm) respectively. The quantitative limits of carbonated beverages were 4.33 mg/L (497 nm) and 4.66 mg/L (628 nm), respectively, ang the quantitative limits of pastries were 14.4 mg/kg (497 nm) and 15.4 mg/kg (628 nm). When measured by the dual - wavelength method, linear range was 0.02 to 1.2 mg/L, the apparent molar absorptivity (κ) was 8.74×104 L/ (mol·cm), the detection limit was 0.014 mg/L. The quantitative limits of carbonated beverages and pastries were 2.33 mg/kg and 7.33 mg/kg, respectively; and the spiked recovery was 97.1% to 103%, the relative standard deviation RSD (n=5) was 2.0% to 2.8%. The method was simple, rapid and sensitive, which was suitable for the determination of benzoic acid in carbonated beverages and pastries.
Key words:  carbonated beverages    pastries    benzoic acid    ethyl violet    dual-wavelength    absorption spectrometry
收稿日期:  2019-08-04                出版日期:  2020-01-25      发布日期:  2020-03-13      期的出版日期:  2020-01-25
基金资助: 重庆市教委科技基金资助项目(KJ1401226);长江师范学院科技基金资助项目(2018CXX117)
作者简介:  学士,教授(本文通讯作者,E-mail:jianghongch@163.com)。
引用本文:    
江虹,庞向东,曾晓莎. 双波长可见吸收光谱法测定糕点及碳酸饮料中的苯甲酸[J]. 食品与发酵工业, 2020, 46(2): 266-271.
JIANG Hong,PANG Xiangdong,ZENG Xiaosha. Determination of benzoic acid in pastries and carbonated beverages by dual- wavelength visible absorption spectrometry[J]. Food and Fermentation Industries, 2020, 46(2): 266-271.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.021898  或          http://sf1970.cnif.cn/CN/Y2020/V46/I2/266
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