Detoxification process of alkali-pretreated solution of corn stover and its application in butanol fermentation
DONG Jinjun1,2*, MA Baojian1,2*, GONG Lei1,2*, HAN Ruizhi1,2*, XU Guochao1,2*, ZHOU Jieyu1,2*, NI Ye1,2*
1 (Laboratory of Biocatalysis and Enzyme Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China) 2 (Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
Abstract: Alkali-pretreated solution of corn stover is one of the main waste water that causes environmental pollution. To reduce the source of pollution, detoxification process of pretreatment solution was developed and applied in butanol fermentation. The lignin in the alkaline pretreatment solution was precipitated and removed by adding sulfuric acid, then the lignin-removed pretreatment solution was detoxified with activated carbon and reused in hydrolysis and fermentation. The optimum pH for precipitating alkali-soluble lignin was 3.8. Alkali pretreatment solution detoxified with different amount of activated carbon had no obvious effect on corn stover hydrolysis, whereas the addition of activated carbon exhibited a significant influence on butanol fermentation. When 15 g/L activated carbon was added, the highest butanol concentration of 10.2 g/L was reached at 72 h. This study provides valuable guidance for utilization of corn stover for waste-free production of biofuels.
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