Abstract: Bacillus velezensis can produce different kinds of antifungal proteins and lipopeptides at the same time. The specific composition and the content of each component in the culture of B. velezensis were greatly influenced by the composition of the medium. In order to obtain the optimal conditions for the production of antifungal components, the effects of different medium compositions and cultivation times on the antifungal activity and components of B. velezensis fermentation broth were investigated, using Aspergillus niger as a pathogenic fungus indicator. As results, the composition of the medium had a significant effect on the total yield and composition of the antifungal components. Landy medium was found to be more beneficial to cell growth, while NB medium was more beneficial to the production of antifungal compounds. The beef extract benefited the production of antifungal compounds, while peptone did not. The produced antifungal compounds were mainly proteins in Landy medium, while those were mainly lipopeptides in NB medium. The optimal medium composition for the accumulation of overall antifungal components were 20 g/L glucose, 5 g/L sodium chloride, 15 g/L beef extract, 5 g/L sodium glutamate, 20 g/L KH2PO4, 10 g/L MgSO4·7H2O, 10 g/L KCl, 3 mg/L FeSO4, 100 mg/L MnSO4, and 3.2 mg/L CuSO4. This condition improved the cell growth by 75.9% compared with that of the Landy medium and increased the overall antifungal activity by 21.9% than that of the NB medium, and the proportion of lipopeptide was higher.
赵鹏鹏,雷淑珍,徐晓光,等. 培养基组成对贝莱斯芽孢杆菌产抑真菌成分的影响[J]. 食品与发酵工业, 2020, 46(5): 147-151.
ZHAO Pengpeng,LEI Shuzhen,XU Xiaoguang,et al. Effect of medium compositions on the production of antifungalcomponents by Bacillus velezensis[J]. Food and Fermentation Industries, 2020, 46(5): 147-151.
 CRISTINA RUIZGARCIA,VICTORIA BEJAR,FERNANDO MARTINEZCHECA,et al.Bacillus velezensis sp. nov. a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain[J]. International Journal of Systematic and Evolutionary Microbiology,2005, 55(1):191-195.  NAM M H,PARK M S,KIM H G,et al.Biological control of strawberry Fusarium wilt caused by Fusarium oxysporum f. sp. fragariae using Bacillus velezensis BS87 and RK1 formulation[J].Journal of Microbiology and Biotechnology, 2009,19(5):520-524.  王哲,曹成亮,左飞,等.杨树变色真菌生防细菌KLBMP033发酵条件优化[J].林业实用技术,2011(11):3-7.  张志焱,赵倩,于佳民,等.一株枯草芽孢杆菌产抗菌肽培养基筛选及发酵工艺优化的研究[J].中国畜牧兽医,2019,46(4):1 217-1 226.  李兴玉,毛自朝,吴毅歆,等.不同培养基对Bacillus subtilis XF-1产脂肽抗生素的影响[J].内蒙古农业大学学报:自然科学版,2014(6):49-53.  李姝江,王淋敏,谯天敏,等.利用响应面法优化贝莱斯芽孢杆菌ZJ20发酵参数[J].西北农林科技大学学报(自然科学版),2019,47(2):94-102.  朱天辉,李姝江,梁漫,等.贝莱斯芽孢杆菌ZJ20菌株及其液体制剂,中国, 2012101226966, [P].2013-12-18.  杨可,司文,林海,等.利用响应面分析法优化贝莱斯芽孢杆菌TCS001的发酵条件[J].农药学学报,2019(4):444-452.