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食品与发酵工业  2020, Vol. 46 Issue (2): 32-39    DOI: 10.13995/j.cnki.11-1802/ts.022229
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
基于高通量测序分析黄酒对D-半乳糖致衰老小鼠模型肠道微生物菌群的影响
刘蓉1,2*, 栾春光2,3, 王德良2,3, 覃思1*, 郝飞克2,3*
1 (湖南农业大学 食品科学技术学院,湖南 长沙,410128)
2 (中国食品发酵工业研究院有限公司,北京,100015)
3 (酒类品质与安全国际联合研究中心,北京,100015)
Effect of Yellow rice wine on intestinal microorganism in D-galactose induced aging mice based on high-throughput sequencing analysis
LIU Rong1,2*, LUAN Chunguang2,3, WANG Deliang2,3, QIN Si1*, HAO Feike2,3*
1 (College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China)
2 (China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China)
3 (International Joint Research Center of Quality and Safety of Alcoholic Beverages, Beijing 100015, China)
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摘要 该文用D-半乳糖(D-galactose,D-gal)颈脊皮下注射建立衰老小鼠模型,评价黄酒对衰老小鼠模型肠道菌群结构的影响。通过设立空白对照、模型组、黄酒组,6周后测定其抗氧化酶活力,同时采用Morris水迷宫对小鼠的认知能力进行测试。收集小鼠粪便,利用IonS5TMXL高通量测序平台对肠道细菌16S rDNA基因进行测序,探索黄酒摄入对衰老小鼠肠道微生物菌群的影响。结果表明,D-gal致衰老小鼠模型的抗氧化相关酶活性明显降低,丙二醛(malondialdehyde,MDA)水平显著增高(P<0.05);黄酒干预后,各剂量组小鼠体内多种抗氧化酶活性升高,MDA含量显著下降(P<0.05);在行为学实验中,适量黄酒干预能够显著提高D-gal致衰老小鼠的认知能力(P<0.05);高通量测序发现黄酒干预显著改善了D-gal致衰老小鼠的肠道菌群结构;进一步分析菌群组成发现,黄酒显著改善了衰老小鼠模型的Firmicutes和Bacteroidetes的相对丰度(P<0.05),显著提升了Bacteroides、Lactobacillus、Bifidobacterium、Lachnospiracea和Butyricicoccus的相对丰度(P<0.05)。适量黄酒摄入可一定程度上改善衰老所致的肠道菌群结构失衡现象,有助于延缓衰老。
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刘蓉
栾春光
王德良
覃思
郝飞克
关键词:  黄酒  D-半乳糖  肠道微生物菌群  菌群分析    
Abstract: The objective of the study is to investigate the effects of Yellow rice wine (YRW) on intestinal microbiota in D-galactose (D-gal) induced aging mice model. The aging model were established with D-gal injection. Fifty Kunming mice were randomly and equally divided into control group, aging model group (model), YRW group. After six weeks, the antioxidant enzyme activity and the content of malondialdehyde (MDA) in the liver and brain of mice were determined. Morris water maze (MWM) was used to test the learning and memory ability of mice in each group. High-throughput sequencing of the 16S rDNA gene of the intestinal flora was performed using an IonS5TMXL platform to explore the effects of YRW of intestinal flora in aging mice. The results showed that the antioxidant enzyme was significantly reduced in liver and brain of D-gal induced aging mice, and MDA contents were increased significantly compare to control group (P & lt;0.05). After the intervention with YRW, the activity of antioxidant enzymes and MDA contents were improved compare to model (P & lt;0.05). Meanwhile, MWM test showed that moderate administration of YRW could significantly improve the cognitive impairment in D-gal induced aging mice (P & lt;0.05). Compared with the control, the relative abundance of Firmicutes and Bacteroidetes were changed significantly in the model group. Compared with the model group, the relative abundance of Firmicutes and Bacteroidetes were improved in the YRW group (P & lt;0.05). At genus level, the proportion of Bacteroide in the aging group was decreased significantly, and the proportions of the Bacteroide, Lactobacillus, Lachnospiracea, Butyricicoccus in YRW group were increased significantly. Moderate intake of YRW could improve the intestinal environment disorder in D-gal induced aging mice, thereby delaying the aging process.
Key words:  Yellow rice wine    D-galactose    intestinal microbiota    analysis of flora
收稿日期:  2019-09-10                出版日期:  2020-01-25      发布日期:  2020-03-13      期的出版日期:  2020-01-25
作者简介:  硕士研究生(覃思副教授和郝飞克高级工程师为共同通讯作者,E-mail:qinsiman@hunau.edu.cn,haofeike@outlook.com)。
引用本文:    
刘蓉,栾春光,王德良,等. 基于高通量测序分析黄酒对D-半乳糖致衰老小鼠模型肠道微生物菌群的影响[J]. 食品与发酵工业, 2020, 46(2): 32-39.
LIU Rong,LUAN Chunguang,WANG Deliang,et al. Effect of Yellow rice wine on intestinal microorganism in D-galactose induced aging mice based on high-throughput sequencing analysis[J]. Food and Fermentation Industries, 2020, 46(2): 32-39.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.022229  或          http://sf1970.cnif.cn/CN/Y2020/V46/I2/32
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