Research progress in functional bacteria in solid-state fermented Baijiu in China
GUO Xuewu1,2,3, FAN Endi2, MA Bingtao2, LI Zexia1, ZHANG Yuhang1, ZHANG Zhimin1, CHEN Yefu2, XIAO Dongguang2*
1(Hebei Hengshui Laobaigan Liquor Group Co. Ltd., Hengshui 053000,China) 2(Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Lab, College of Biotechnology of Tianjin University of Science and Technology, Tianjin 300457, China) 3(Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry,Yibin 644007,China)
Abstract: Bacteria are one of the three major microorganisms for Baijiu fermentation. Bacteria that play important roles in the production of Baijiu includes: Bacillus, lactic acid bacteria, and clostridium etc. They produce trace amount of components such as esters, organic acids, pyrazines, terpenes. These components affect the flavor and quality of Baijiu. Because of the important functions of bacteria in Baijiu, and with the advancement in microbial isolation and culturing techniques, as well as in analytical detection techniques, researches on Baijiu fermentation have become more and more in-depth. This paper summaries recent research progress of functional bacteria in Baijiu, and introduces the main functional bacteria and their functions in Baijiu production, aiming to provide reference for future studies of functional bacteria in Baijiu.
ZHENG X W, HAN B Z. Baijiu, Chinese liquor: History, classification and manufacture[J]. Journal of Ethnic Foods, 2016,3(1):19-25.
[2]
LIANG H P, LI W F, LUO Q C, et al. Analysis of the bacterial community in aged and aging pit mud of ChineseLuzhou-flavour liquor by combined PCR-DGGE and quantitative PCR assay[J]. Journal of the Science of Food and Agriculture, 2015,95(13):2 729-2 735.
[3]
GOU M, WANG H Z, YUAN H W, et al. Characterization of the microbial community in three types of fermentation starters used for Chinese liquor production[J]. Journal of the Institute of Brewing, 2015,121(4):620-627.
[4]
WANG C, SHI D, GONG G. Microorganisms in Daqu: a starter culture of Chinese Maotai-flavor liquor[J]. World Journal of Microbiology and Biotechnology, 2008,24(10):2 183-2 190.
[5]
WANG X D, BAN S D, HU B D, et al. Bacterial diversity of Moutai-flavour Daqu based on high-throughput sequencing method[J]. Journal of the Institute of Brewing, 2017,123(1):138-143.
[6]
FAN G S, SUN B G, FU Z L et al. Analysis of physicochemical indices, volatile flavor components, and microbial community of a Light-Flavor Daqu[J]. Journal of the American Society of Brewing Chemists, 2018,76(3):209-218.
[7]
ZOU W, ZHAO C Q, LUO H B. Diversity and function of microbial community in Chinese strong-flavor Baijiu ecosystem: a Review[J]. Frontiers in Microbiology, 2018,9:671.
[8]
ZHENG X W, YAN Z, HAN B Z, et al. Complex microbiota of a Chinese “Fen” liquor fermentation starter (Fen-Daqu), revealed by culture-dependent and culture-independent methods[J]. Food Microbiology, 2012,31(2):293-300.
[9]
ZHANG H X, ZHANG X H, ZHAO J L, et al. Analysis of the microbial communities of three kinds of Fen-Daqu by PLFAs[J]. Journal of the Institute of Brewing, 2016,122(1):34-41.
[10]
ZHENG X W, YAN Z, NOUT M J R, et al. Characterization of the microbial community in different types of Daqu samples as revealed by 16S rRNA and 26S rRNA gene clone libraries[J]. World Journal of Microbiology & Biotechnology, 2015,31(1):199-208.
[11]
LUO H B, WEI C H, YUAN X L. Study on the metabolites of a bacteria strain producing Esterifying synthetase[J]. Advanced Materials Research, 2012,1914(1103):1 080-1 084.
[12]
WANG D L, CHEN L, GAO L P, et al. Cultivable bacterial diversity and amylase production in two typical light-flavor Daqus of Chinese spirits[J]. Frontiers in Life Science, 2015,8(3):264-270.
[13]
LI H, LIAN B, DING Y H, et al. Bacterial diversity in the central black component of Maotai Daqu and its flavor analysis[J]. Annals of Microbiology, 2014,64(4):1 659-1 669.
[14]
ZHANG R, WU Q, XU Y. Lichenysin, a cyclooctapeptide occurring in Chinese liquor jiannanchun reduced the headspace concentration of phenolic off-flavors via hydrogen-bond interactions[J]. Journal of Agricultural and Food Chemistry, 2014,62(33):8 302-8 307.
[15]
ZHU B F, XU Y, FAN W L. High-yield fermentative preparation of tetramethylpyrazine by Bacillus sp using an endogenous precursor approach[J]. Journal of Industrial Microbiology & Biotechnology, 2010,37(2):179-186.
[16]
ZHI Y, WU Q, DU H, et al. Biocontrol of geosmin-producing Streptomyces spp. by two Bacillus strains from Chinese liquor[J]. International Journal of Food Microbiology, 2016,231:1-9.
WU H, ZHANG S, MA Y, et al. Comparison of microbial communities in the fermentation starter used to brew Xiaoqu liquor[J]. Journal of the Institute of Brewing, 2017,123(1):113-120.
WANG J, ZHONG Q, YANG Y, et al. Comparison of bacterial diversity between two traditional starters and the Round-Koji-Maker starter for traditional cantonese Chi-Flavor Liquor brewing[J]. Frontiers in Microbiology, 2018,9:1 053.
SILO-SUH L A, LETHBRIDGE B J, RAFFEL S J, et al. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85[J]. Applied & Environmental Microbiology, 1994,60(6):2 023-2 030.
[23]
QURESHI N, EZEJI T C, EBENER J, et al. Butanol production by Clostridium beijerinckii. Part I: Use of acid and enzyme hydrolyzed corn fiber[J]. Bioresource Technology, 2008,99(13):5 915-5 922.
[24]
STEIN T. Bacillus subtilis antibiotics: structures, syntheses and specific functions[J]. Molecular Microbiology, 2005,56(4):845-857.
[25]
ZHAO C Q, YAN X L, YANG S T, et al. Screening of Bacillus strains from Luzhou-flavor liquor making for high-yield ethyl hexanoate and low-yield propanol[J]. LWT-Food Science and Technology, 2017,77(10):60-66.
[26]
YAN Z, ZHENG X E, CHEN J U, et al. Effect of different Bacillus strains on the profile of organic acids in a liquid culture of Daqu[J]. Journal of the Institute of Brewing, 2013,119(1-2):78-83.
GUO J, JIA S. Effect of cellulase-producing bacteria on enzyme activity and ester production in fermented grains of Chinese Liquor[J]. Journal of the American Society of Brewing Chemists, 2018,73(2):130-136.
[39]
GAO W J, FAN W L, XU Y. Characterization of the key odorants in light aroma type Chinese Liquor by gas chromatography-olfactometry, quantitative measurements, aroma recombination, and omission studies[J]. Journal of Agricultural and Food Chemistry, 2014,62(25):5 796-5 804.
[40]
FAN W L, XU Y, ZHANG Y H. Characterization of pyrazines in some Chinese Liquors and their approximate concentrations[J]. Journal of Agricultural and Food Chemistry, 2007,55(24):9 956-9 962.
[41]
YANG Z. Antimicrobial compounds and extracellular polysaccharides produced by lactic acid bacteria: structures and properties[M]. Z. Yang, 2000.
[42]
LIANG H P, LUO Q C, ZHANG A, et al. Comparison of bacterial community in matured and degenerated pit mud from ChineseLuzhou-flavour liquor distillery in different regions[J]. Journal of the Institute of Brewing, 2016,122(1):48-54.
[43]
ZHAO J S, ZHENG J, ZHOU R Q, et al. Microbial community structure of pit mud in a Chinese strong aromatic liquor fermentation pit[J]. Journal of the Institute of Brewing, 2012,118(4):356-360.
[44]
CHAI L J, XU P X, QIAN W, et al. Profiling the Clostridia with butyrate-producing potential in the mud of Chinese liquor fermentation cellar[J]. International Journal of Food Microbiology, 2019,297:41-50.
[45]
LIU C L, HUANG D, LIU L Y, et al. Clostridium swellfunianum sp. nov., a novel anaerobic bacterium isolated from the pit mud of Chinese Luzhou-flavor liquor production[J]. Antonie Van Leeuwenhoek: Journal of Microbiology and Serology, 2014,106(4):817-825.
[46]
HU X L, DU H, XU Y. Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production[J]. International Journal of Food Microbiology, 2015,214:116-122.
[47]
ZHANG W X, QIAO Z W, SHIGEMATSU T, et al. Analysis of the bacterial community in Zaopei during production of Chinese Luzhou-flavor Liquor[J]. Journal of the Institute of Brewing, 2005,111(2):215-222.
[48]
LIU M K, ZHAO K, TANG Y M, et al. Analysis of Clostridium cluster I community diversity in pit mud used in manufacture of Chinese Luzhou-flavor liquor[J]. Food Science and Biotechnology, 2015,24(3):995-1 000.
[49]
ZHENG Q, LIN B, WANG Y, et al. Proteomic and high-throughput analysis of protein expression and microbial diversity of microbes from 30-and 300-year pit muds of Chinese Luzhou-flavor liquor[J]. Food Research International, 2015,75(Sep.):305-314.
2020, Vol. 46 
