Abstract: Many methods, such as titration, electrochemical and continuous spectrophotometry have been developed for measuring the activity of glucose oxidase. However, they are regarded as impractical since they all have large errors in independent repeated experiments and a simple and fast method with better reproducibility and less costs is desperately needed. In this study, an iodine-starch spectrophotometry method for measuring the activity of glucose oxidase was developed. The method used β-D-glucose as the substrate which could be turned into gluconic acid and produce hydrogen peroxide at the same time when the reaction started and an iodine-starch spectrophotometry method was used to measure the content of hydrogen peroxide produced. The results showed that the new developed method had good parallelism within the activity range of 0.1-0.5 U/mL in the measurement system. The average relative deviation was less than 5%, which was within the statistical margin of error. To sum up, this innovative method for measuring glucose oxidase activity is promising in laboratory or industry application for its accurate and stable with good reproducibility and low cost.
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