Differentially expressed gene function and mevalonate metabolism analysis of recombinant Saccharomyces cerevisiae strain N6076
WANG Xuan1, OU Ke1, FENG Guangwen1, CHEN Fuxin2, WANG Ting3, MAMATRISHAT Mamat1, QIAN Weidong3, MAO Peihong1*
1(Research Center of Ion Beam Biotechnology,College of Physics Science and Technology,Xinjiang University,Urumqi 830046,China) 2(College of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China) 3(School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’an 710021, China)
Abstract: In order to understand the information of differentially expressed genes (DEGs) and the metabolism of mevalonate(MVA) in recombinant Saccharomyces cerevisiae strain N6076 at different fermentation phases, based on transcriptomic sequencing and MVA assay, its functions of DEGs at three fermentation time points (0 h, 48 h, 96 h) were analyzed and compared. The results of gene ontology (GO) terms showed that 14 biological processes (BP), 2 cell components (CC) and 2 molecular functions (MF) were added, involving 1 140 DEGs in recombinant strain N6076. The results of Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that there were 13 new metabolic pathways involving 77 DEGs, of which 5 DEGs were related to the new increased terpenoid skeleton biosynthesis pathway in recombinant strain N6076. The results of mevalonate kinase (MVK) gene expression and quantitative analysis of MVA by liquid chromatography-tandem mass spectrometry (LC-MS/MS) showed that the change trend of MVK gene expression was consistent with that of MVA production during the whole fermentation process. The results can provide a basis for further understanding the gene expression and MVA metabolism regulation in recombinant yeast strain N6076.
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2020, Vol. 46 
