Identification of a Bacillus amyloliquefaciens strain KHQH-1 and preliminary study on its antibacterial activity
FU Zhiying1*, CHEN Xiaobo1, PAN Jieru2, DENG Youjin3, LAI Tengqiang1, LIU Caizhen1
1(Fujian Vocational College of Agriculture, Fuzhou 350119, China) 2(Fuzhou Center for Disease Control and Prevention, Fuzhou 350004, China) 3(Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002, China)
Abstract: In order to find the microbial resources with antiseptic and fresh-keeping function, a strain of bacteria with antibacterial activity was isolated and purified from the pervious orange fresh-keeping study. The strain was identified as Bacillus amyloliquefaciens KHQH-1 by morphology, biochemistry and molecular biology. Through BLAST alignment on NCBI platform, sequence analysis and phylogenetic tree construction with Clustalw and Mega 7.0 software, the phylogenetic status of the strain was established. Point planting method and filter paper method were used to study the antagonistic effects of KHQH-1 on several common fungi and bacteria in food. The results showed that KHQH-1 had a certain inhibitory effect on Rhizopus spp. and Aspergillus oryzae. It had a strong inhibitory effect on the most common fungi in food, such as Aspergillus flavus, Penicillium spp. and Trichoderma viride. The inhibitory circles were 13.70, 9.30 and 10.87 mm, respectively. KHQH-1 has certain inhibitory effect on common bacteria Staphylococcus aureus and Escherichia coli in food, and its inhibitory circle was 3.20 and 8.20 mm, respectively. These results will provide some theoretical and technical reference, which can explore the preservation technology of natural products and the development and application of microbial preparations.
付志英,陈晓波,潘洁茹,等. Bacillus amyloliquefaciens KHQH-1的鉴定及其抗菌活性初步研究[J]. 食品与发酵工业, 2020, 46(6): 89-94.
FU Zhiying,CHEN Xiaobo,PAN Jieru,et al. Identification of a Bacillus amyloliquefaciens strain KHQH-1 and preliminary study on its antibacterial activity[J]. Food and Fermentation Industries, 2020, 46(6): 89-94.
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2020, Vol. 46 
