Abstract: The maltotriose-forming α-amylase (AmyA) can produce maltotriose-rich syrup from starch or maltodextrin, and has broad application prospects in food and beverage industry. The maltotriose-forming α-amylase derived from Thermobifida fusca NTU22 (referred as TfAmyA) was recombinantly expressed in Bacillus subtilis WS11 for the first time. The recombinant TfAmyA was further purified and characterized. The optimal temperature of the TfAmyA is 55 ℃, the optimal pH is 6.0. The optimal conditions for maltotriose production from maltodextrin catalyzed by the recombinant TfAmyA were also investigated. The results showed that when 15% (mass concentration) maltodextrin (DE 5-7) was used as the substrate, the optimal conditions for enzymatic conversion were temperature 55 ℃ and pH 5.5. The dosage of TfAmyA is 60 U/g substrate, and the dosage of pullulanase was 32 U/g substrate. Under these conditions, the maximum conversion rate of maltotriose was 44.4% when the reaction proceeded to 11 h. These results indicated that the recombinant TfAmyA has superior properties and provides a theoretical basis for the industrial preparation of maltotriose.
胡凡,宿玲恰,吴敬. Thermobifida fusca麦芽三糖淀粉酶的重组表达及其在麦芽三糖制备中的应用[J]. 食品与发酵工业, 2020, 46(5): 23-30.
HU Fan,SU Lingqia,WU Jing. Recombinant expression of maltotriose-forming alpha-amylases fromThermobifida fusca and its application in preparation of maltotriose[J]. Food and Fermentation Industries, 2020, 46(5): 23-30.
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2020, Vol. 46 
