Abstract: In order to elucidate the effect of the peroxidase EfeB on the regulation of intracellular oxidative stress in Escherichia coli, Red recombination/disruption and over-expression of the efeB gene were performed. The results showed that after over-expression of efeB, the content of malondialdehyde in the cell was decreased by 85% compared with the parental strain (223.79 nmol/g protein), and the level of intracellular reactive oxygen species was 1.36 folds compared with that of the parental strain. Meanwhile, the expression level of katE was decreased 87.62%, while efeU, efeO, sodA, oxyR and recA up-regulated by 3.88, 1.33, 2.48, 1.95 and 1.49 folds respectively. After exogenous addition of H2O2, katE was further down-regulated by 94.81% while recA was further up-regulated by 8.09 folds in the efeB over-expressing strain Eco/pEE. Conversely, efeB-deficient strain exhibited slower growth rate with higher intracellular malondialdehyde content (1.15-fold of the parental strain). The level of intracellular reactive oxygen species in efeB-deficient strain was 0.5-fold of the parental strain. The results provided new physiological function of EfeB in E.coli under oxidative stress.
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DING Liangliang,LIU Jinsheng,GU Pengshuai,et al. The role of peroxidase EfeB in Escherichia coli under cell oxidative stress[J]. Food and Fermentation Industries, 2020, 46(17): 33-39.
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