Combined mutagenesis of ARTP and 5-BU for improving production ofadenosine in Bacillus subtilis
YANG Xinping1, SONG Ci1, ZHANG Weihao1, LIU Yan1,2, WANG Zhou1,2, XUE Zhenglian1,2*
1(College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China) 2(Anhui Engineering Technology Research Center of Microbial Fermentation, Wuhu 241000, China)
Abstract: The mutagenesis of Bacillus subtilis B-0 was carried out using atmospheric and room temperature plasma (ARTP) and 5-bromouracil (5-BU), and the adenosine high-yield strain was selected by sulfaguanidine (SG) resistance and in 48-well plates. A genetic stable mutant strain B-6 with 35% higher yield of adenosine than that of Bacillus subtilis B-0 was finally obtained after 6 cycles of mutagenesis and screening. Briefly, the combination of ARTP and 5-BU mutagenesis combined with 48-well plates can significantly improve the breeding efficiency and accuracy of adenosine high-yielding strains, and also provide a theoretical reference for the selection of other nucleoside strains.
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2020, Vol. 46 
