Lactobacillus salivarius CCFM 1054alleviates the infection ofCampylobacter jejuni in mice by regulating the gut microbiota
JIN Xing1, HE Yufeng1, ZHOU Yonghua2*, CHEN Xiaohua3, WANG Gang1*, ZHAO Jianxin1, ZHANG Hao1, CHEN Wei1
1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China) 2(Key Laboratory of Parasitic Disease Prevention and Control Technology for National Health andHealth Commission,Wuxi 214064, China) 3(College of Life Science and Environment, Hengyang Normal University, Hengyang 421008, China)
Abstract: In this study, the biological characteristics of Lactobacillus salivarius CCFM 1054 in vitro were evaluated, such as the ability of acid production, the antibacterial ability of free supernatant for Campylobacter jejuni growth, the tolerance to artificial gastrointestinal fluids, the inhibition to co-cultured C. jejuni, the adhesion to HT-29 cell and the ability of biofilms generation. It was founded that CCFM 1054 had strong acid-producing ability and could inhibit the growth of C. jejuniin vitro. At the same time, it had good tolerance under artificial simulated gastrointestinal fluid, high adhesion to HT-29 cells and strong self-film-forming ability. The L. rhamnosus LGG and L. plantarum N49 were used as control strains to CCFM 1054 when administered to C. jejuni and Toxoplasma co-infected mice by gavage. The results showed that CCFM 1054 could significantly change the composition of gut microbiota, such as Firmicutes, Bacteroidetes and Proteobacteria at the phylum level and Campylobacter, Lactobacillus, Pediococcus, Faecoccus, Coprococcus and Unclassified Enterobacteriaceae at the genera level. Changes in gut microbes reduced the colonization of C. jejuni in mice and alleviated the infection of C. jejuniin vivo. The correlation between the changes of gut microbiota and the biological characteristics of lactic acid bacteria in vitro indicated that CCFM 1054's high adhesion to HT-29 cell and strong biofilm-forming ability made it change the gut microbiota in mice significantly.
金星,贺禹丰,周永华,等. 唾液乳杆菌CCFM 1054通过改变肠道菌群缓解空肠弯曲杆菌在小鼠体内的感染[J]. 食品与发酵工业, 2020, 46(5): 1-8.
JIN Xing,HE Yufeng,ZHOU Yonghua,et al. Lactobacillus salivarius CCFM 1054alleviates the infection ofCampylobacter jejuni in mice by regulating the gut microbiota[J]. Food and Fermentation Industries, 2020, 46(5): 1-8.
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