Abstract: In this study, DSS-induced colitis mice were used to investigate the effects of two Lactobacillus rhamnosus strains on disease and their potential mechanisms. C57BL/6J mice with colitis induced by 25 g/L DSS were administered with L. rhamnosus strains for 28 d. The results showed that L. rhamnosus FZJTZ46L6 alleviated DSS-induced colitis significantly and more effectively than that by FNMGEL5-1. L. rhamnosus FZJTZ46L6 alleviated the body weight loss caused by DSS, improved stool consistency and relieved bloody stool, and significantly improved the colonic mucosal damage in mice. Although FNMGEL5-1 alleviated the symptoms of diseases caused by DSS, it showed no effect on colonic inflammation and pathological damage. In addition, compared with the model group, L. rhamnosus FZJTZ46L6 significantly increased the transcription level of tight junction proteins in colon and reduced pro-inflammatory cytokines such as TNF-α、IL-1β、IL-6 and IFN-γ in colon. Besides, the content of short-chain fatty acids in the feces of mice was also increased significantly. The abundance of genera Coprococcus, the propionate and butyrate-producing bacteria, were elevated together with the diversity of gut microbiota.
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