Abstract: This research aimed to evaluate the application of conjugated linoleic acid (CLA) as a functional component in formula food for patients with colitis, and probiotics can play an important role in the colon by producing CLA. Therefore, these ingredients were added to nutritional formulas to explore their effects on mice with chronic ulcerative colitis (UC). 50 specific pathogen free (SPF) C57BL/6J mice (male, 8 weeks, 18-20 g) were randomly divided into control group, experimental group including nutrition formula group B, C(including 1% CLA), P(containing 1×109 CFU/mL probiotics). Mice were administered 20-30 g/L dextran sulfate sodium (DSS) in their drinking water for three cycles to induce chronic colitis except the control group. Then the experimental groups were given sterilized water and fed nutrition formula B, C, P ad libitum for 2 weeks. The body weight, colon length, myeloperoxidase (MPO), pathological sections, inflammatory factors, and microbial composition were used as evaluation indicators to assess the effects of different dietary supplements on the recovery of mice with UC. Results showed that all the dietary supplements were beneficial against UC compared with the DSS-induced mice. Among them, diet with CLA or probiotic had better effect on colon length, cytokine expression body weight, MPO, and gut microbial composition, respectively. Nutrition formula diet in this research was beneficial to UC and the effect of diet with CLA or probiotic were better.
 吴佳红,史圣华,莫日根.溃疡性结肠炎的治法进展[J].内蒙古中医药,2016,35(12):133-134.  NG S C,SHI H Y,HAMIDI N,et al.Worldwide incidence and prevalence of inflammatory bowel disease in the 21 st century:a systematic review of population-based studies[J].The Lancet,2017,390(10 114):2 769-2 778.  HENGSTERMANN S,VALENTINI L,SCHAPER L,et al.Altered status of antioxidant vitamins and fatty acids in patients with inactive inflammatory bowel disease[J].Clin Nutr,2008,27(4):571-578.  王威.炎症性肠病的营养支持治疗方案回顾与疗效分析[J].中国现代药物应用,2019,13(23):49-50.  LEVINE A,SIGALL BONEH R,WINE E.Evolving role of diet in the pathogenesis and treatment of inflammatory bowel diseases[J].Gut,2018,67(9):1 726-1 738.  YUAN G,CHEN X,LI D.Modulation of peroxisome proliferator-activated receptor gamma (PPAR γ) by conjugated fatty acid in obesity and inflammatory bowel disease[J].Journal of Agricultural and Food Chemistry,2015,63(7):1 883-1 895.  朱诗雅,翟齐啸,赵星,等.不同乳杆菌缓解慢性酒精性肝损伤的作用比较[J].食品与发酵工业,2019,45(22):20-26.  BASSAGANYA-RIERA J,VILADOMIU M,PEDRAGOSA M,et al.Probiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage PPAR γ to suppress colitis[J].PloS One,2012,7(2):31238.  WIRTZ S,POPP V,KINDERMANN M,et al.Chemically induced mouse models of acute and chronic intestinal inflammation[J].Nat Protoc,2017,12(7):1 295-1 309.  YAN S,YANG B,ZHAO J,et al.A ropy exopolysaccharide producing strain Bifidobacterium longum subsp.longum YS108R alleviates DSS-induced colitis by maintenance of the mucosal barrier and gut microbiota modulation[J].Food Funct,2019,10(3):1 595-1 608.  王俊通.产共轭亚油酸乳酸菌对DSS诱导小鼠结肠炎的改善作用[D].无锡:江南大学,2016.  俞赟霞.产细菌素乳酸菌对小鼠肠道微生态的影响[D].无锡:江南大学,2018.  REES V.Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines[J].Clinical & Experimental Immunology,1998,114(3):385-391.  FANG Z,LI L,LIU X,et al.Strain-specific ameliorating effect of Bifidobacterium longum on atopic dermatitis in mice[J].Journal of Functional Foods,2019,60:103 426.  COAKLEY M,ROSS R P,NORDGREN M,et al.Conjugated linoleic acid biosynthesis by human‐derived Bifidobacterium species[J].Journal of Applied Microbiology,2003,94(1):138-145.  GASSULL M A,CABRÉ E.Nutrition in inflammatory bowel disease[J].Current Opinion in Clinical Nutrition & Metabolic Care,2001,4(6):561-569.  YANG B,CHEN H,GAO H,et al.Bifidobacterium breve CCFM683 could ameliorate DSS-induced colitis in mice primarily via conjugated linoleic acid production and gut microbiota modulation[J].Journal of Functional Foods,2018,49:61-72.  FOSSIEZ F,DJOSSOU O,CHOMARAT P,et al.T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines[J].Journal of Experimental Medicine,1996,183(6):2 593-2 603.  CHASSAING B,AITKEN J D,MALLESHAPPA M,et al.Dextran sulfate sodium (DSS)-induced colitis in mice[J].Curr Protoc Immunol,2014,104(1):15.25.1-15.25.14.  YOUNG D,IBUKI M,NAKAMORI T,et al.Soy-derived di-and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis[J].J Nutr,2012,142(2):363-368.  SU C G,WEN X,BAILEY S T,et al.A novel therapy for colitis utilizing PPAR-γ ligands to inhibit the epithelial inflammatory response[J].The Journal of Clinical Investigation,1999,104(4):383-389.  CHEN Y,YANG B,ROSS R P,et al.Orally Administered CLA Ameliorates DSS-Induced Colitis in Mice via Intestinal Barrier Improvement,Oxidative Stress Reduction,and Inflammatory Cytokine and Gut Microbiota Modulation[J].Journal of Agricultural and Food Chemistry,2019,67(48):13 282-13 298.  WU H,YE L,LU X,et al.Lactobacillus acidophilus alleviated salmonella-induced goblet cells loss and colitis by notch pathway[J].Molecular Nutrition & Food Research,2018,62(22):1 800 552.  HU Q,YUAN B,WU X,et al.Dietary intake of Pleurotus eryngii ameliorated dextran-sodium-sulfate-induced colitis in mice[J].Molecular Nutrition & Food Research,2019,63(17):1 801 265.  LI J,CUI H,CAI Y,et al.Tong-xie-yao-fang regulates 5-HT level in diarrhea predominant irritable bowel syndrome through gut microbiota modulation[J].Frontiers in Pharmacology,2018,9:1 110.  ZHENG J,ZHEANG J,GUO Y,et al.Improvement on metabolic syndrome in high fat diet-induced obese mice through modulation of gut microbiota by Sangguayin decoction[J].Journal of Ethnopharmacology,2020,246:112 225.  JU T,KONG J Y,STOTHARD P,et al.Defining the role of Parasutterella,a previously uncharacterized member of the core gut microbiota[J].The ISME Journal,2019,13(6):1 520-1 534.  毛立新,郭元春,钱剑.双歧杆菌三联活菌胶囊治疗溃疡性结肠炎的临床观察[J].中国药房,2015,26(36):5 095-5 096.