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食品与发酵工业  2020, Vol. 46 Issue (17): 80-86    DOI: 10.13995/j.cnki.11-1802/ts.023653
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
代谢多种低聚糖乳酸菌的筛选鉴定及其部分益生特性研究
林丽, 邓倩, 罗琳, 康渝接, 严唯玮, 何利, 敖晓琳, 刘书亮*
四川农业大学 食品学院,四川 雅安,625014
Screening and identification of lactic acid bacteria utilizing various oligosaccharides and their partial prebiotic properties
LIN Li, DENG Qian, LUO Lin, KANG Yujie, YAN Weiwei, HE Li, AO Xiaolin, LIU Shuliang*
College of Food Science, Sichuan Agriculture University, Ya’an 625000, China
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摘要 探究了110株乳酸菌对4种低聚糖(低聚木糖、低聚果糖、菊粉和低聚异麦芽糖)的广谱代谢能力,筛选获得3株对4种低聚糖代谢能力强的乳酸菌,对其进行系统鉴定和益生特性研究。结果表明,仅8株乳酸菌不能代谢低聚糖,其余菌株可代谢1~4种低聚糖,其中可代谢4种低聚糖的菌株SNR24为发酵乳杆菌(Lactobacillus fermentum)、菌株RP30和PC140为植物乳杆菌(Lactobacillus plantarum)。这3株菌能在以低聚糖为唯一碳源的培养基中良好生长,在11~13 h内进入稳定期,低聚糖添加量与生长量并非呈线性增长关系,菌株SNR24、RP30和PC140的最适宜低聚糖种类及添加质量浓度分别为50 g/L菊粉、30 g/L低聚异麦芽糖和50 g/L菊粉;对pH 3.0的低酸环境和质量浓度2 g/L胆盐环境耐受能力较好;在人工模拟胃肠液中的存活率为16%~70%;3株菌对多种抗生素敏感,对链霉素、环丙沙星耐药,菌株RP30和PC140对庆大霉素、四环素也耐药。综上所述,3株菌具有益生菌的潜质。
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林丽
邓倩
罗琳
康渝接
严唯玮
何利
敖晓琳
刘书亮
关键词:  乳酸菌  低聚木糖  低聚果糖  低聚异麦芽糖  菊粉  益生特性    
Abstract: 110 strains of suspected lactic acid bacteria were investigated for their broad-spectrum utilization to four oligosaccharides (xylo-oligosaccharides, fructo-oligosaccharides, inulin, isomalto-oligosaccharides). Of which, 102 strains of lactic acid bacteria could use at least one oligosaccharide. Three strains, SNR24, RP30 and PC140 with maximum utilization of four oligosaccharides were screened out and identified as Lactobacillus fermentum, Lactobacillus plantarum and L. plantarum. Their prebiotic characteristics were also investigated. The results showed that the three strains grew well in the media with oligosaccharides as the only carbon source, and entered the stable period within 11-13 h. However, the oligosaccharide concentration was not linearly associated with the growth of lactic acid bacteria. The optimal oligosaccharides and concentrations for SNR24, RP30 and PC140 were 50 g/L of inulin, 30 g/L of isomalto-oligosaccharides and 50 g/L of inulin, respectively. The three strains were tolerant to low-acid environment of pH 3.0 and bile salt environment of 2 g/L, and their survival rate in artificial simulated gastrointestinal fluid was 16%-70%. They were sensitive to multiple antibiotics, and resistant to streptomycin and ciprofloxacin. RP30 and PC140 were also resistant to gentamicin and tetracycline. In summary, they had the potential to become probiotics.
Key words:  lactic acid bacteria    xylo-oligosaccharides    fructo-oligosaccharides    isomalto-oligosaccharides    inulin    prebiotic properties
收稿日期:  2020-02-16      修回日期:  2020-03-27           出版日期:  2020-09-15      发布日期:  2020-10-14      期的出版日期:  2020-09-15
基金资助: 四川省科技厅重点研发项目(2019YFN0110);四川农业大学本科生科研兴趣计划;四川省眉山市科技局重点研发项目
作者简介:  本科生(刘书亮教授为通讯作者,E-mail:lsliang999@163.com)
引用本文:    
林丽,邓倩,罗琳,等. 代谢多种低聚糖乳酸菌的筛选鉴定及其部分益生特性研究[J]. 食品与发酵工业, 2020, 46(17): 80-86.
LIN Li,DENG Qian,LUO Lin,et al. Screening and identification of lactic acid bacteria utilizing various oligosaccharides and their partial prebiotic properties[J]. Food and Fermentation Industries, 2020, 46(17): 80-86.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.023653  或          http://sf1970.cnif.cn/CN/Y2020/V46/I17/80
[1] XU X, XU P, MA C,et al.Gut microbiota, host health, and polysaccharides[J].Biotechnology Advances,2013,31(2):318-337.
[2] 柳青.具有潜在益生特性乳酸菌的筛选鉴定及其特性研究[D].呼和浩特:内蒙古农业大学, 2018.
[3] CARMODY R N, GERBER G K, LUEVANO J M, et al.Diet dominates host genotype in shaping the murine gut microbiota[J].Cell Host & Microbe,2015,17(1):72-84.
[4] GIBSON G R, ROBERFEOID M B.Dietary modulation of the human colonic microbiota-introducing the concept of prebiotics[J].Journal of Nutrition,1995,125(6):1 401-1 412.
[5] 耿铁柱.低聚糖对益生菌饮料活菌数量的影响[D].重庆:西南大学,2016.
[6] 罗予,孟林敏,毛理纳,等.低聚果糖体内外对肠道菌的影响[J].中国微生态学杂志,2003(6):10-11.
[7] 谷雪玲,吕宏伟,宋泽和,等.低聚异麦芽糖对乳酸杆菌增殖及产酸效果的研究[J].家畜生态学报,2018,39(9):43-45.
[8] SAULNIER D M, MOLENAAR D, VOS W M, et al.Identification of prebiotic fructo-oligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays[J].Applied and Environmental Microbiology,2007,73(6):1 753-1 765.
[9] 王苗,蒋敏,李恒,等.乳酸菌对魔芋甘露低聚糖的降解与利用[J].食品与发酵工业,2016,42(11):20-24.
[10] BANUELOS O, FERMANDEZ L, CORRAL J M, et al.Metabolism of prebiotic products containing beta(2-1) fructan mixtures by two Lactobacillus strains[J].Anaerobe,2008,14(3):184-189.
[11] JAMES A, WANG Y S.Characterization, health benefits and applications of fruits and vegetable probiotics[J].Cyta-Journal of Food, 2019,17(1):770-780
[12] 王莉. K-B纸片扩散法药敏试验技术要点[J].医药前沿,2013(25):59-60.
[13] R.E.步坎南, N.E.吉本斯伯, 等.伯杰细菌鉴定手册[M].第八版.北京:科学出版社,1984:797-822.
[14] 刘宏宇,汪立平,艾连中,等.乳酸菌的抗氧化活性和耐酸耐胆盐性能的研究[J].食品工业科技,2014,35(2):92-96;99.
[15] 胡爱华,敖晓琳,陈岑,等.乳酸菌耐酸耐胆盐机制的研究进展[J].食品工业科技,2015,36(8):380-383;389.
[16] 李禤,贾丹,刘军龙,等.新分离植物乳杆菌的药敏性和抑菌性试验[J].中国兽医科学,2019,49(7):879-886.
[17] 刘思思.乳酸菌利用低聚糖的特性及在肠道中的作用[D].无锡:江南大学,2015.
[18] IGNATOVA T, ILIEV I, KIRILOV N, et al.Effect of oligosaccharides on the growth of Lactobacillus delbrueckii subsp bulgaricus strains isolated from dairy products[J].Journal of Agricultural and Food Chemistry,2009,57(20):9 496-9 502.
[19] 毛丙永.功能性低聚糖对肠道细菌的影响及机制[D].无锡:江南大学,2015.
[20] 曾小群,潘道东,郭宇星,等.不同来源乳酸菌的药敏试验[J].中国食品学报,2012,12(9):179-185.
[21] CATALOLUK O, GOGEBAKAN B.Presence of drug resistance in intestinal Lactobacilli of dairy and human origin in Turkey[J].FEMS Microbiology Letters,2004(236):7-12.
[22] JACOBSEN L, WILCKS A, HAMMER K, et al.Horizontal transfer of tet (M) and erm (B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats[J].FEMS Microbiology Ecology, 2007,59(1):158-166.
[23] 陈臣,卢艳青,于海燕,田怀香.乳酸菌代谢低聚糖机理的研究进展[J].中国食品学报,2019,19(6):274-283.
[24] ROSSI M, CORRADINI C, AMARETTI A, et al.Fermentation of fructo-oligosaccharides and inulin by bifidobacteria:A comparative study of pure and fecal cultures[J].Applied and Environmental Microbiology,2005(71):6 150-6 158.
[25] OSE R, HIRANO K, MAENO S, et al.The ability of human intestinal anaerobes to metabolize different oligosaccharides:Novel means for microbiota modulation?[J].Anaerobe,2018,51:110-119.
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