Screening of lipopeptide-producing strains from fairy soybean and the characteristic of its lipopeptide products
CHEN Zhina1,2*, XUE Yongzhen1, YE Tao1,2, LIU Tian1, SHEN Yeqiao1
1(College of Biological Engineering, Huainan Normal University, Huainan 232038, China) 2(Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes, Huainan 232038, China)
Abstract: After preliminarily isolated from Anhui traditional fermented soybean food--fairy soybean, by blood-agar method, five strains were further examined by oil spreading, inhibitory test and detection of genes related to lipopeptide synthesis, and one strain BS6-2 producing Surfactin was screened out. Strain BS6-2 was identified as Bacillus subtilis by 16S rDNA gene sequencing analysis. The metabolite of BS6-2 was preliminarily identified as lipopeptide by Fourier transform infrared spectrometry (FT-IR). Using HPLC and ultra-performance liquid chromatography-electron spray ionization-quadrupole-time-of-flight/mass spectrometry (UPLC-ESI/Q-TOF MS) and compared with standards, the lipopeptide was further determined as three Surfactin homologues. The possible sequence was β-OH-C13-15-Glu-Leu-Leu-Val-Asp-Leu-Leu(Ile). The Surfactin lipopeptides produced by strain BS6-2 showed stable emulsification capability and also exhibited antifungal activity against Aspergillus niger. These results suggest that B. subtilis BS6-2 has potential application prospect.
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2020, Vol. 46 
