Study on the stability of 98 pesticide standards in three common solvents
ZHANG Weibiao1,2, GU Yaping1,2*, ZENG Dingling1,2, WANG Rui1,2, LIN Huichun1,2, YIN Yijuan1,2, FANG Changfa3
1 Shenzhen Centre of Inspection and Testing for Agricultural Products, Shenzhen 518040, China 2 Key Laboratory of Supervision for Edible Agricultural Products, Administration for Market Regulation of Guangdong Province, Shenzhen 518040, China 3 School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, China
摘要 研究QuEChERS-液相色谱串联四极杆质谱(liquid chromatography tandem quadrupole mass spectrometry,LC-MS-MS)方法中,乙腈、乙腈+水(体积比1∶1)和甲醇+水(体积比1∶1)3种常用的溶剂对98种农药标准物质和2种加标基质稳定性的影响。标准溶液(浓度为0.080 μg/mL)11 h的连续测定结果显示,甲醇+水(体积比1∶1)中标准物质峰面积长时间的稳定性最好,乙腈+水(体积比1∶1)次之,乙腈最差。使用甲醇+水(体积比1∶1)配制标准物质时,长期不稳定的农药仅为四螨嗪、氟苯脲、杀扑磷和异菌脲。对韭菜和橙子2种加标基质放置3 h的稳定性研究结果表明,橙子基质中农药测定结果在3种溶剂中稳定性均优于韭菜,而以甲醇+水(体积比1∶1)作为溶剂对于检测结果的稳定性贡献更大。
Abstract: The stability of 98 pesticide standards and two matrix standards in three commonly used solvents, namely acetonitrile, acetonitrile/water (1∶1), and methanol/water (1∶1), was tested using QuEChERS-LC-MS-MS. The continuous measurement results of the standards for 11 hours showed that the peak area of the standards in methanol/water showed relative long-term stability than that in acetonitrile/water; while the stability in acetonitrile was poor. When methanol/water was used to prepare the standards, tetrabenazine, flufenuron, fenflufen, and isocarbazone were to be unstable. The unstable pesticides in methanol/water were tetramazine, flubenzuron, fenflufen, and allazurea. The stability results of the two matrix standards for 3 hours in leeks and oranges indicated that pesticide stability in the orange matrix was better, and methanol/water contributed more to the stability of the test results.
张伟彪,顾亚萍,曾定玲,等. 三种常用溶剂对98种农残检测结果稳定性的影响[J]. 食品与发酵工业, 2020, 46(16): 236-242.
ZHANG Weibiao,GU Yaping,ZENG Dingling,et al. Study on the stability of 98 pesticide standards in three common solvents[J]. Food and Fermentation Industries, 2020, 46(16): 236-242.