Abstract: Temporin-SHf is a kind of small molecular peptide with broad-spectrum antimicrobial activity, the expression of Temporin-SHf in Pichia pastoris could provide reference for its industrial production. The pPIC9K-Temporin-SHf expression vector was constructed and transformed into P. pastoris GS115 strain, which was then verified by PCR and RT-PCR, and the induction conditions were optimized and antibacterial activity was analyzed in vitro. The results showed that the antimicrobial peptide Temporin-SHf was expressed successfully in P. pastoris, which had antibacterial activity on Staphylococcus aureus and Escherichia coli strains. And the optimum expression conditions of Temporin-SHf were as follows: temperature 28 ℃, initial induction OD600=1.5, adding 0.5% (volume fraction) methanol, and induced for 72 h. In this study, a novel antimicrobial peptide Temporin-SHf was expressed in P. pastoris for the first time and the induction conditions were optimized, which provided theoretical base for the industrial production.
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