Abstract: To obtain a fast fermentation yeast from natural starter,high-throughput screening was performed using natural fermentation broth of grape,kefir grains and kombucha.The selected strain was subjected to tolerant domestication and fermentation optimization.OD600 of the fermented broth increased by 2.2 times at 300 g/L glucose and 8.5 times at 2.0 g/L NaCl after domestication.The strain was identified as Saccharomyces cerevisiae and named as Saccharomyces cerevisiae LBBE-1.Additionally,medium optimization was performed in shaking flasks and the optimal medium (glucose 55 g/L,corn slurry 21 g/L,yeast extract 4 g/L) was used for 15 L bioreactor fermentation.Finally,the OD600 reached 92.9 in 22 h.The viable cell count was 6.3×109 CFU/mL.The results laid a certain foundation for the application of natural yeast in baked goods.
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