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食品与发酵工业  2020, Vol. 46 Issue (17): 277-284    DOI: 10.13995/j.cnki.11-1802/ts.024121
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曾慧君, 付诗慧, 晏涛, 杨小平, 刘萍, 徐玮, 董秋花, 郦娟*
武汉食品化妆品检验所,湖北 武汉,430012
Research advances of oligonucleotide aptamer-based biosensor for foodborne pathogen detection
ZENG Huijun, FU Shihui, YAN Tao, YANG Xiaoping, LIU Ping, XU Wei, DONG Qiuhua, LI Juan*
Wuhan Institute for Food and Cosmetic Control,Wuhan 430012,China
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摘要 核酸适配体是一小段经体外筛选得到的寡核苷酸序列,因具备易合成、易修饰、特异性强、稳定性高且广泛结合各种靶标分子等优点受到广泛关注。文章综述了近几年核酸适配体在食源性致病菌检测的研究进展,介绍了核酸适配体的筛选和作用原理,列举了已经筛选获得的食源性致病菌适配体,总结了9种适配体生物传感器在食源性致病菌检测中的应用,提出了目前该技术仍存在的问题,并展望了核酸适配体生物传感器在食源性致病菌检测领域的未来发展趋势。
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关键词:  核酸适配体  生物传感器  食源性致病菌    
Abstract: Aptamers are single-stranded DNA or RNA molecules, which can specifically recognize and bind to their targets. Due to its simplicity of synthesis, simplicity of modification, strong specificity, high stability and wide range of various target molecules, it had been applied in many fields such as food safety, disease diagnosis and public health. This article reviewed the development in the field of aptamer-based biosensor for foodborne pathogen detection, focused on the classification of aptamer screening technologies and nine aptamer-based biosensors application technologies, including colorimetric aptasensors, fluorescence aptasensors, chemiluminescence aptasensors, surface-enhanced Raman scattering aptasensors, surface plasmon resonance aptasensors, flow cytometry aptasensors, lateral flow aptasensors, electrochemical aptasensors and quartz crystal microbalance aptasensors. Although significant progress has been achieved in the field in the last decade, challenges or drawbacks in the applications still remain to be overcame. Lastly, the new trends about aptamer-based biosensors for the foodborne pathogen were also discussed.
Key words:  aptamer    biosensor    foodborne pathogen
收稿日期:  2020-04-07      修回日期:  2020-04-24           出版日期:  2020-09-15      发布日期:  2020-10-14      期的出版日期:  2020-09-15
基金资助: 湖北省自然科学基金计划项目(2017CFB366);湖北省自然科学基金计划项目(2018CFB547)
作者简介:  曾慧君博士研究生和付诗慧硕士研究生为共同第一作者(郦娟高级工程师为通讯作者,
曾慧君,付诗慧,晏涛,等. 核酸适配体生物传感器在食源性致病菌检测中的应用[J]. 食品与发酵工业, 2020, 46(17): 277-284.
ZENG Huijun,FU Shihui,YAN Tao,et al. Research advances of oligonucleotide aptamer-based biosensor for foodborne pathogen detection[J]. Food and Fermentation Industries, 2020, 46(17): 277-284.
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