Optimization of ultrasonic assisted degreasing process of rabbit bone meal
XU Jingbing1,2, LI Xue1,3, HE Zhifei1,4*, MAO Qing2*
1(College of Food Science,Southwest University, Chongqing 400715, China) 2(Chongqing Institute for Food and Drug Control, Chongqing 401121, China) 3(Agro-product Storage and Processing Institute, Chongqing Academy of Agricultural Science, Chongqing 401329, China) 4(Chongqing Engineering Research Center of Regional Food, Chongqing 400715, China)
Abstract: In order to improve the degreasing rate of rabbit bone meal, the parameters of ultrasonic assisted solvent degreasing process were optimized with rabbit limb bone as raw material. In this study, the degreasing agent was selected by degreasing rate and protein retention rate, and the ultrasonic assisted degreasing parameters were optimized by single factor and Box-Behnken response surface experiment. The degreasing rates of rabbit bone meal under different technological conditions were compared and studied, and the production validation test was carried out finally. Results showed that ethyl acetate is the best degreaser. By single factor experiment, the optimum conditions of ultrasonic assisted degreasing were liquid to material ratio of 5∶1(g∶g), ultrasonic power of 360 W, ultrasonic time of 15 min. On the basis of single factor, the parameters of ultrasonic assisted degreasing were optimized by response surface method, and the quadratic regression equation was established, the optimal parameters of ultrasonic assisted degreasing process of rabbit bone were obtained finally, which were liquid to material ratio of 5.3∶1(g∶g), ultrasonic power of 480 W, ultrasonic time of 24 min, the degreasing rate of rabbit bone meal was (94.63±0.20)%, the relative error with the theoretical prediction value was less than 5%, which will have certain guiding significance for the application of animal bone meal.
 廖洪波,李洪军,李铁牛,等.动物骨粉的营养、加工及应用[J].肉类工业,2003(1):23-25.  TRAYLOR S L,CROMWELL G L,LINDEMANN M D.Bioavailability of phosphorus in meat and bone meal for swine [J].Journal of Animal Science,2005,83(1):1 054-1 061.  蔡蕊.湿法超细粉碎鸡骨泥品质及加工特性研究[D].广州:华南理工大学,2012.  陈丽尧.猪骨骼超微粉碎工艺及新产品的开发[D].哈尔滨:东北农业大学,2008.  刘丽莉,马美湖,杨协力.畜禽骨骼开发利用的研究进展[J].肉类工业,2008(9):50-53.  LINDER M,FANNI J,PARMENTIER M,et al.Protein recovery from veal bones by enzymatic hydrolysis[J].Food Science,1995,60:949-952.  张春晖.骨源食品加工技术[M].北京:科学出版社,2015.  REZAEE A,RANGKOOY H,KHAVANIN A,et al.High photocatalytic decomposition of the air pollutant formaldehyde using nano-ZnO on bone char[J].Environmental Chemistry Letters,2014,12(2):353-357.  JIA Puqi,TAN Hongwei,LIU Kuiren,et al.Enhanced photocatalytic performance of ZnO bone char composites[J].Materials Letters,2017,205:233-235.  ANTONELLA D Z,ZSOLT S.The role of rabbit meat as functional food[J].Meat Science,2011,88(3):319-331.  任灿,贺稚非,李杨梅,等.超细微兔骨理化性质的研究[J].食品与发酵工业,2017,43(2):226-231.  李少博,李洪军,贺稚非.纳米级兔骨粉球磨法制备工艺优化[J].农业工程学报,2017,33(14):300-306.  李少博,贺稚非,邓大川,等.不同粒度兔骨粉营养特性和理化特性的研究[J].食品与发酵工业,2017,43(10):74-79.  LI Shaobo,HE Zhifei,LI Hongjun.Effect of nano-scaled rabbit bone powder on physicochemical properties of rabbit meat batter [J].Journal of the Science of Food and Agriculture,2018,98(12):4 533-4 541.  任灿.超微兔骨与高钙兔糜凝胶的制备及其理化特性研究[D].重庆:西南大学,2017.  GB 5009.5—2016 食品中蛋白质的测定[S].北京:中国标准出版社,2016.  GB 5009.3—2016 食品中水分的测定[S].北京:中国标准出版社,2016.  GB 5009.6—2016 食品中脂肪的测定[S].北京:中国标准出版社,2016.  刘文媛,贾伟,李侠,等.超声辅助水热法提取牛骨油工艺参数优化[J].农业工程学报,2018,34(8):283-290.  秦晓洁,沈青山,贾伟,等.超声辅助脱脂对牦牛骨粉制备及其理化特性的影响[J].农业工程学报,2018,34(21):272-280  肖厚荣,杨红,汪珊珊.牛骨粉品质及其生物利用率研究[J].食品研究与开发,2016,37(12):5-9.  TIAN Yuting,XU Zhenbo,ZHENG Baodong,et al.Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil[J].Ultrasonics Sonochemistry,2013,20(1):202-208.  李晔,马良,张宇昊,等.正交试验优化鱼皮超声乳化法脱脂工艺[J].食品科学,2012,33(24):127-131.  徐向宏,何明珠.试验设计与Design-Expert、SPSS应用[M].北京:科学出版社,2010:150-158.  宁俊丽,龙霞,黄先智,等.响应面法优化鸭油超声波提取工艺[J].食品与发酵工业,2019,45(8):184-190.