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食品与发酵工业  2020, Vol. 46 Issue (22): 28-35    DOI: 10.13995/j.cnki.11-1802/ts.024343
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
邝嘉华, 黄燕燕, 胡金双, 余佳佳, 周钦育, 赵珊, 刘冬梅*
(华南理工大学 食品科学与工程学院,广东 广州,510640)
Optimization of fermentation conditions and anti-oxidation of exopolysaccharideproduced by Bacillus amyloliquefaciens DMBA-K4
KUANG Jiahua, HUANG Yanyan, HU Jinshuang, YU Jiajia, ZHOU Qinyu, ZHAO Shan, LIU Dongmei*
(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)
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摘要 从云南普洱茶中分离出1株产胞外多糖(exopolysaccharide,EPS)的菌株,经16S rDNA鉴定,确定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)DMBA-K4;利用单因素和响应面法优化其胞外多糖的产量,并对其体外抗氧化活性进行研究。经优化后,DMBA-K4产胞外多糖的最佳发酵条件是:4 g/L 蔗糖、5 g/L胰蛋白胨、10 g/L酵母提取粉、5 g/L NaCl、培养时间18 h,pH 7.6,接种量3.10%;在此条件下,其胞外多糖产量为171.31 mg/L,较原始条件提高155.7%。抗氧化实验结果表明,DMBA-K4所产的胞外多糖具有较强的抗氧化活性。当EPS质量浓度为5 mg/mL时,其1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率达78.60%,对·OH的清除率为75.47%。该研究可为微生物胞外多糖的工业生产提供理论支持,丰富新型微生物多糖的应用前景。
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关键词:  解淀粉芽孢杆菌  胞外多糖  发酵条件  响应面优化  抗氧化    
Abstract: An exopolysaccharides (EPS) producing strain was isolated from Yunnan Puer tea.This strain was defined as Bacillus amyloliquefaciens DMBA-K4 by 16S rDNA.Single factor trials and response surface tests were performed to optimize its EPS yield,and its in vitro antioxidant activity was also investigated.The optimal fermentation conditions were as follow:the culture medium consisted of 4 g/L sucrose,5 g/L tryptone,10 g/L yeast,5 g/L NaCl,pH 7.6,inoculator amount of 3.10% and fermented for 18 h.Under these conditions,the maximal yields of EPS increased by 155.7% and reached 171.31mg/L.In vitro antioxidant results showed that EPS had strong antioxidant activity.When the concentration of EPS was 5 mg/mL,the scavenging rate of DPPH free radical and ·OH was 78.60% and 75.47%,respectively.The research provide theoretical support to EPS industrialization and widen the utilization prospect of novel bio-polysaccharide.
Key words:  Bacillus amyloliquefaciens    exopolysaccharide    fermentation conditions    response surface    antioxidation
收稿日期:  2020-04-29      修回日期:  2020-05-26           出版日期:  2020-11-25      发布日期:  2020-12-11      期的出版日期:  2020-11-25
基金资助: 国家自然科学基金(31771908);广州市科技计划项目(201903010015)
作者简介:  硕士研究生(刘冬梅教授为通讯作者,
邝嘉华,黄燕燕,胡金双,等. 解淀粉芽孢杆菌DMBA-K4高产胞外多糖的发酵条件优化及其抗氧化活性研究[J]. 食品与发酵工业, 2020, 46(22): 28-35.
KUANG Jiahua,HUANG Yanyan,HU Jinshuang,et al. Optimization of fermentation conditions and anti-oxidation of exopolysaccharideproduced by Bacillus amyloliquefaciens DMBA-K4[J]. Food and Fermentation Industries, 2020, 46(22): 28-35.
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