Abstract: An exopolysaccharides (EPS) producing strain was isolated from Yunnan Puer tea.This strain was defined as Bacillus amyloliquefaciens DMBA-K4 by 16S rDNA.Single factor trials and response surface tests were performed to optimize its EPS yield,and its in vitro antioxidant activity was also investigated.The optimal fermentation conditions were as follow:the culture medium consisted of 4 g/L sucrose,5 g/L tryptone,10 g/L yeast,5 g/L NaCl,pH 7.6,inoculator amount of 3.10% and fermented for 18 h.Under these conditions,the maximal yields of EPS increased by 155.7% and reached 171.31mg/L.In vitro antioxidant results showed that EPS had strong antioxidant activity.When the concentration of EPS was 5 mg/mL,the scavenging rate of DPPH free radical and ·OH was 78.60% and 75.47%,respectively.The research provide theoretical support to EPS industrialization and widen the utilization prospect of novel bio-polysaccharide.
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