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食品与发酵工业  2021, Vol. 47 Issue (1): 125-131    DOI: 10.13995/j.cnki.11-1802/ts.024501
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
嗜酸乳杆菌La28对丙戊酸暴露引起的子代大鼠外周炎症和肝损伤的缓解作用
孔庆敏, 朱慧越, 田培郡, 赵建新, 张灏, 陈卫, 王刚*
(江南大学 食品学院,江苏 无锡,214122)
Alleviation effects on peripheral inflammation and liver damage by Lactobacillus acidophilus La28 in offspring rats induced by valproic acid exposure
KONG Qingmin, ZHU Huiyue, TIAN Peijun, ZHAO Jianxin, ZHANG Hao, CHEN Wei, WANG Gang*
(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
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摘要 为评价嗜酸乳杆菌La28对丙戊酸(valproic acid,VPA)暴露引起的子代大鼠外周炎症和肝损伤的作用,同时明确其对VPA大鼠肠道菌群的调节作用。在大鼠孕期12.5 d腹腔注射VPA和生理盐水,断奶后,随机选取雄性子代分成对照组、VPA组、La28组和利培酮(MED)组(n=5),La28组每天灌胃菌液(109 CFU/mL),对照、VPA和MED组灌胃等量生理盐水和药物,干预期28 d。干预结束后,评价外周炎症、肝功能和肠道菌群的改善情况。与VPA组大鼠相比,嗜酸乳杆菌La28干预能显著地改善VPA大鼠的外周炎症和肝功能,其中对IL-6、TNF-α、ALT和GGT的作用效果更为显著。此外,嗜酸乳杆菌La28可能通过上调AllobaculumheMucispirillum的相对丰度,增加厚壁菌门丰度,降低拟杆菌门丰度从而改善VPA暴露引起子代大鼠的神经炎症和肝功能。
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孔庆敏
朱慧越
田培郡
赵建新
张灏
陈卫
王刚
关键词:  嗜酸乳杆菌La28  丙戊酸  外周炎症  肝功能  肠道菌群    
Abstract: To evaluate the effects of Lactobacillus acidophilus La28 on relieving peripheral inflammation and improving liver function in rats induced by valproic acid, Wistar rats were intraperitoneally injected with VPA or normal saline on day 12.5 after pregnancy and the male offspring were randomly grouped as the control group, VPA group, La28 group and risperidone (MED) group (n=5). La28 group was treated with L. acidophilus La28 (109 CFU/mL) by gavage for 28 days. The control, VPA and MED groups were treated with equivalent saline and risperidone by gavage. After the intervention, peripheral inflammation, liver function and gut microbiota were evaluated. Compared with rats in VPA group, L. acidophilus La28 significantly improved peripheral inflammation and liver function in rats. The effects on IL-6, TNF-α, alanine aminotransferase (ALT) andγ-glutamyl transpeptidase (GGT) were more significant. In addition, L. acidophilus La28 might improve the peripheral inflammation and liver function through regulating the relative abundance of Allobaculumhe and Mucispirillum, increasing Firmicutes abundance, and decreasing Bacteroides abundance.
Key words:  Lactobacillus acidophilus La28    valproic acid    peripheral inflammation    liver function    gut microbiota
收稿日期:  2020-05-20      修回日期:  2020-07-15                发布日期:  2021-02-03      期的出版日期:  2021-01-15
基金资助: 国家自然科学基金项目(31972052);中央高校基本科研业务费专项资金项目(JUSRP22006)
作者简介:  博士研究生(王刚副教授为通讯作者,E-mail:wanggang@jiangnan.edu.cn)
引用本文:    
孔庆敏,朱慧越,田培郡,等. 嗜酸乳杆菌La28对丙戊酸暴露引起的子代大鼠外周炎症和肝损伤的缓解作用[J]. 食品与发酵工业, 2021, 47(1): 125-131.
KONG Qingmin,ZHU Huiyue,TIAN Peijun,et al. Alleviation effects on peripheral inflammation and liver damage by Lactobacillus acidophilus La28 in offspring rats induced by valproic acid exposure[J]. Food and Fermentation Industries, 2021, 47(1): 125-131.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.024501  或          http://sf1970.cnif.cn/CN/Y2021/V47/I1/125
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