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食品与发酵工业  2021, Vol. 47 Issue (1): 236-242    DOI: 10.13995/j.cnki.11-1802/ts.024509
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四大产区莼菜黄酮类物质组成分析及抗氧化、抑菌活性研究
韩芳1, 李京凌1, 袁婧1, 于杰1,2*
1(西南大学 园艺园林学院,重庆,400715)
2(南方山地园艺学教育部重点实验室,重庆,400715)
Composition analysis and antioxidant and antibacterial activities of flavonoids of Brasenia schreberi in four main areas
HAN Fang1, LI Jingling1, YUAN Jing1, YU Jie1,2*
1(College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China)
2(Key Laboratory of Horticulture Science for Southern Mountain Regions, Ministry of Education, Chongqing 400715, China)
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摘要 为研究莼菜黄酮类物质组成及抗氧化、抑菌体外功能活性,利用紫外-可见分光光度法和高效液相色谱法分别测定中国四大产区莼菜总黄酮、黄酮类物质组成及含量,2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS),1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH),铁还原/抗氧化能力(ferric reducing/antioxidant power,FRAP)三种抗氧化法评价各产区莼菜黄酮抗氧化能力,滤纸片扩散法测定莼菜黄酮对变形杆菌(Proteus vulgaris)、大肠杆菌(Escherichia coli)和铜绿假单胞菌(Pseudomonas aeruginosa)的抑菌效果。结果显示:石柱莼菜总黄酮含量最高,为(42.505±0.458) mg/g,西湖莼菜最低,为(15.845±0.119) mg/g;供试材料中共检测到7种黄酮类物质,主要为异鼠李素、槲皮素和山奈酚3种;各产区莼菜均具有较好的抗氧化活性及不同程度的抑菌活性,且其大小变化顺序一致,均为:石柱莼菜﹥利川莼菜﹥雷波莼菜﹥西湖莼菜。该结果为莼菜黄酮类物质的进一步研究提供参考。
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韩芳
李京凌
袁婧
于杰
关键词:  莼菜  黄酮类  抗氧化  抑菌  高效液相色谱    
Abstract: In order to study the composition of water shield flavonoids and their antioxidant and antibacterial activities in vitro, in this study, the composition and content of flavonoid in water shield from four major producing areas in China were determined by ultraviolet-visible spectrophotometry (UV-VIS) and high performance liquid chromatography (HPLC).Three antioxidant methods, DPPH, ABTS and FRAP were used to evaluate the antioxidant capacity of flavonoids in water shield from various regions.The filter paper diffusion method was used to determine the antibacterial effects of flavonoids from water shield on Proteus vulgaris, Escherichia coli and Pseudomonas aeruginosa.The results showed that the content of total flavonoids in Shizhu water shield was the highest (42.505±0.458) mg/g, and West Lake was the lowest (15.845±0.119) mg/g.A total of 7 flavonoids were detected in the test materials, mainly isorhamnetin, quercetin and kaempferol.Water shield in each production area has good antioxidant activity and different degrees of antibacterial activity, and the order of their size changes is the same:all are:Shizhu﹥Lichuan﹥Leibo﹥West Lake.This will provide reference data for further research on water shield flavonoids.
Key words:  Brasenia schreberi    flavonoids    antioxidation    antibacterial    HPLC
收稿日期:  2020-05-21      修回日期:  2020-07-20                发布日期:  2021-02-03      期的出版日期:  2021-01-15
基金资助: 国家自然科学基金(31772260);重庆市社会民生项目(cstc2017shms-kjfp80009);西南大学科技创新基金(SZ201802)
作者简介:  硕士研究生(于杰副教授为通讯作者,E-mail:yujie1982@swu.edu.cn)
引用本文:    
韩芳,李京凌,袁婧,等. 四大产区莼菜黄酮类物质组成分析及抗氧化、抑菌活性研究[J]. 食品与发酵工业, 2021, 47(1): 236-242.
HAN Fang,LI Jingling,YUAN Jing,et al. Composition analysis and antioxidant and antibacterial activities of flavonoids of Brasenia schreberi in four main areas[J]. Food and Fermentation Industries, 2021, 47(1): 236-242.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.024509  或          http://sf1970.cnif.cn/CN/Y2021/V47/I1/236
[1] 吴征镒,RAVEN P H.中国植物志[M].北京:科学出版社,2001.
WU Z Y,RAVEN P H.Flora of China[M].Beijing:Science Press,2001.
[2] LI Z Z,GICHIRA A W,WANG Q F,et al.Genetic diversity and population structure of the endangered basal angiosperm Brasenia schreberi (Cabombaceae) in China[J].PeerJ,2018,6(5):1-14.
[3] KIM H,WANG Q,SHOEMAKER C F,et al.Polysaccharide gel coating of the leaves of Brasenia schreberi lowers plasma cholesterol in hamsters[J].Journal of Traditional and Complementary Medicine,2015,5(1):56-61.
[4] 吴洪梅, 吕泽芳,张昭,等.四大莼菜产区商品成分种类和质量分数测定及比较研究[J].西南大学学报(自然科学版),2017,39(5):76-82.
WU H M,LV Z F,ZHANG Z,et al.A comparative study of the commercially valuable components of water shield (Brasenia schreberi) from 4 cultivation areas in China[J].Journal of Southwest University (Natural Science Edition),2017,39(5):76-82.
[5] LIU H,HUANG W,HUANG L,et al.Research progress on resources and nutritional components of Brasenia schreberi[J].Botanical Research,2019,8(1):7-14.
[6] 王慧芳, 谈如蓝.莼菜的药用价值与综合开发应用[J].现代食品科技,2016,7(13):45-46.
WANF H F,TAN R L.Water shield medicinal values and comprehensive development and application[J].Modern Food,2016,7(13):45-46.
[7] 李燕, 柯剑鸿,焦大春,等.莼菜的营养价值及其应用研究进展[J].长江蔬菜,2018,18(14):36-39.
LI Y,KE J H,JIAO D C,et al.The nutritional value of Brasenia schreberi and its application research progress[J].Journal of Changjiang Vegetables,2018,18(14):36-39.
[8] ALEKSANDRA K,DOROTA S W.Flavonoids-food sources and health benefits[J].Roczniki Państwowego Zakńadu Higieny,2014,68(2):79-85.
[9] HUGHES S D,KETHEESAN N,HALEAGRAHARA N.The therapeutic potential of plant flavonoids on rheumatoid arthritis[J].Critical Reviews in Food Science and Nutrition,2017,57(17):3 601-3 613.
[10] KIM C,NA H R,CHOI H K.Conservation genetics of endangered Brasenia schreberi based on RAPD and AFLP markers[J].Journal of Plant Biology,2008,51(4):260-268.
[11] OH M J,NA H R,CHOI H K,et al.High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of water shield (Brasenia schreberi)[J].Plant Biotechnology Reports,2008,2(1):87-92.
[12] XIE C,LI J,PAN F,et al.Environmental factors influencing mucilage accumulation of the endangered Brasenia schreberi in China[J].Scientific Reports,2018,8(1):583-597.
[13] XIAO H,CAI X,FAN Y,et al.Antioxidant activity of water-soluble polysaccharides from Brasenia schreberi[J].Pharmacognosy Magazine,2016,12(47):193-197.
[14] LEGAULT J,PERRON T,MSHVILDADZE V,et al.Antioxidant and anti-inflammatory activities of quercetin 7-O-β-D-glucopyranoside from the leaves of Brasenia schreberi[J].Journal of Medicinal Food,2011,14(10):1 127-1 134.
[15] LEE M K,PARK H J,KWON S H,et al.Tellimoside,a flavonol glycoside from Brasenia schreberi, inhibits the growth of cyanobacterium (Microcystis aeruginosa LB 2385)[J].Journal of the Korean Society for Applied Biological Chemistry,2013,56(1):117-121.
[16] SHIMODA H,NAKAMURA S,HITOE S,et al.Anti-Adipogenic polyphenols of water shield suppress TNF-α-Induced cell damage and enhance expression of HAS2 and HAPB2 in Adiponectin-Treated dermal fibroblasts[J].Natural Products Chemistry & Research,2014,2(5):146-153.
[17] 许瑞波, 曹晓英,王明艳,等.睡莲叶黄酮的提取及其抑菌活性研究[J].食品科技,2009,34(4):190-192;197.
XU R B,CAO X Y,WANG M Y,et al.Studies on the extracting technology of flavones from pygmy waterlily leaves and their antibacterial activity[J].Food Science and Technology,2009,34(4):190-192;197.
[18] GOMEZ J D,VATTUONE M A,ISLA M I,et al.Antioxidant activities of Sechium edule(Jacq.) Swartz extracts[J].Food Chemistry,2006,97(3):452-458
[19] 左龙亚. 柑橘亚属植物果皮多酚类物质提取及其抗氧化、抑菌活性检测[D].重庆:西南大学,2018.
ZUO L Y.Polyphenols extraction and antioxidant,antifungal activities test of subgenus Citrus[D].Chongqing:Southwest University,2018.
[20] GORINSTEIN S,HARUENKIT R,PARK Y S,et al.Bioactive compounds and antioxidant potential in fresh and dried Jaffa® sweeties,a new kind of Citrus fruit[J].Journal of the Science of Food and Agriculture,2004,84(12):1 459-1 463.
[21] ALMEIDA M M B,SOUSA P H M D,ARRIAGA A M C,et al.Bioactive compounds and antioxidant activity of fresh exotic fruits from northeastern Brazil[J].Food Research International,2011,44(7):2 155-2 159.
[22] BENZIE I F F,STRAIN J J.The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”:The FRAP assay[J].Analytical Biochemistry,1996,239(1):70-76.
[23] 吕爽, 田呈瑞,韩莎莎.薄荷属植物精油和多酚类的抑菌性及抗氧化性研究[J].食品与生物技术学报,2011,30(6):827-831.
LV S,TIAN C R,HAN S S.Study on antibacterial and antioxidant activity of essential oils and polyphenol in mentha plants[J].Journal of Food and Biotechnology,2011,30(6):827-831.
[24] 孙雨欣, 毛水芳,陈银宁,等.莼菜体外胶的分离及其体外功能活性研究[J].食品与发酵工业,2020,46(2):55-60.
SUN Y X,MAO S F,CHEN Y N,et al.Effect of ultrasonic treatment on the separation,physical properties of mucilage from Brasenia schreberi[J].Food and Fermentation Industries,2020,46(2):55-60.
[25] 唐莹, 黄先智,丁晓雯.食源性黄酮类化合物对肝脏功能的影响与作用机制研究进展[J].食品与发酵工业,2018,44(2):284-289.
TANG Y,HUANG X Z,DING X W.Research progress of the effects of dietary flavonoids on liver function and its mechanisms[J].Food and Fermentation Industries,2018,44(2):284-289.
[26] 刘馨, 郭婷,肖洁,等.莼菜贮藏保鲜的研究进展[J].农业机械,2012,10(30):111-113.
LIU X,GUO T,XIAO J,et al.Research progress on storage and fresh-keeping of Brasenia schreberi[J].Farm Machinery,2012,10(30):111-113.
[27] 蔡敏, 李长春,毕宏光,等.荷叶黄酮的提取和纯化工艺研究[J].湖北农业科学,2015,54(22):5 691-5 693;5 697.
CAI M,LI C C,BI H G,et al.Study on extraction and purification process of flavonoids from lotus leaf[J].Hubei Agricultural Sciences,2015,54(22):5 691-5 693;5 697.
[28] 李鹏程. 王莲类黄酮成分分析[D].南京:南京农业大学,2017.
LI P C.The flavonoid composition in Victoria[D].Nanjing:Nanjing Agricultural University,2017.
[29] 袁丽萍, 王卫,吉莉莉,等.荷叶中黄酮抑菌作用的研究进展[J].山东化工,2018,47(22):46-47.
YUAN L P,WANG W,JI L L,et al.Research progress on the bacteriostatic effect of flavonoids in lotus leaves[J].Shandong Chemical Industry,2018,47(22):46-47.
[30] 董兰芳, 张琴,童潼,等.方格星虫多糖抗菌和抗氧化活性研究[J].广西科学,2013,20(4):289-293.
DONG L F,ZHANG Q,TONG T,et al.Study on the antibacterial and antioxidant activities of polysaccharide from Sipunculus nudus[J].Guangxi Science,2013,20(4):289-293.
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