LI Xia1, CHEN Haiou1, HAN Shufang1, LU Fengying1, ZHOU Yuheng2, SHAN Yang3, LI Jing1*
1(College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541006,China) 2(Guangxi Institute of Botany,Guilin 541006,China) 3(Hunan Academy of Agricultural Sciences Agricultural Product Processing Institute,Changsha 410125,China)
Abstract: To explore the potential of carboxymethyl xylan as a prebiotics,we modified by sodium hydroxide chloroacetic acid reaction system was used to carboxymethylate xylan,and the structure was characterized by infrared spectroscopy.Its anti-digestibility was explored by simulating the human digestive environment,and its prebiotic effects on four probiotics (Lactobacillus brevis,Lactobacillus plantarum, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophiles) were studied.The results showed that carboxymethyl xylan had characteristic absorption peak at 1 609 cm-1、1 405 cm-1 and 1 320 cm-1,which indicated that it was successfully modified and the degree of substitution was 0.68.In the digestion experiments of simulated saliva,gastric juice and intestinal fluid,the hydrolysis degree of carboxymethyl xylan was lower than 9%,5% and 6%,respectively,indicating its resistance to digestion in human digestive tract.Moreover,carboxymethyl xylan could promote the growth of four probiotics and the promoting effect increased with its concentration.The optimum concentration of carboxymethyl xylan for the growth and reproduction of probiotics was 3%,reaching the maximum growth within 24-36 hours.Carboxymethyl xylan has the potential to be used as prebiotics.
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