Abstract: In order to make full use of pomelo resources and reduce environmental pollution, pomelo peel was used as the raw material and an improved Osborne method was used to extract proteins in stages. The structure of the protein was identified by scanning electron microscope, infrared spectrum and amino acid composition analysis. And the survival rate of mouse RAW 264.7 macrophages, NO content, and the expression level of IL-6 and TNF-α were detected by MTT, and ELISA detection, respectively. It was found that the extraction rates of albumin, globulin, gliadin, and glutenin were (15.47±0.17)%, (4.20±0.09)%, (0.18±0.02)% and (0.04±0.01)%, respectively. The results of scanning electron microscope showed that the albumin was in the form of a sheet and the globulin was in a porous form, while the gliadin was in a strip-like structure. Moreover, the conformations of the three proteins only changed at the functional groups of N—H, —OH, and —CH. Furthermore, the content of glycine and alanine were the most abundant which reached 125.49 g/100 g and 50.48 g/100 g respectively. Albumin had the strongest ability to stimulate RAW 264.7 cells to produce NO and TNF-α and IL-6 at 800 μg/mL, at 95.46 μmol/L, 9 528.05 pg/mL and 1 769.33 pg/mL, respectively. The experimental data showed that pomelo peel albumin had the strongest immune activity, suggesting its potential as a health food development and medical drug.
林诺怡,成坚,王琴,等. 柚皮蛋白的结构表征及细胞免疫活性初步研究[J]. 食品与发酵工业, 2021, 47(3): 59-65.
LIN Nuoyi,CHENG Jian,WANG Qin,et al. A preliminary study on structure and cellular immune activity of pomelo peel protein[J]. Food and Fermentation Industries, 2021, 47(3): 59-65.
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2021, Vol. 47 
