Abstract: Monacolin K (MK, also known as lovastatin), produced by fermenting steamed rice with Monascus sp.,is a beneficial secondary metabolite which has hypocholesterolemic effects. While some Monascus strains can produce the mycotoxin citrinin, which gives rise to a controversy over the safety of Monascus fermented products. In this study, 11 Monascuspurpureus and 10 Monascusruber strains were selected, and the contents of MK and citrinin in red yeast rice produced by these strains were analyzed with high performance liquid chromatography (HPLC). All 11 M. purpureus strains and 4 M. ruber strains could produce citrinin, while a M. purpureus strain and 9 M. ruber strains could produce MK. The contents of MK and citrinin in fermented rice produced by 5 M. ruber strains met the standards of functional red yeast rice (QB/T 2847—2007). The results from this study provide valuable information for modifying standards of related functional red yeast rice.
李志强,刘颖,林风,等. 两种红曲菌固态发酵产莫纳可林K和桔霉素的比较[J]. 食品与发酵工业, 2020, 46(17): 94-98.
LI Zhiqiang,LIU Ying,LIN Feng,et al. Comparison of monacolin K and citrinin production by two Monascus species in red yeast rice[J]. Food and Fermentation Industries, 2020, 46(17): 94-98.
[1] 林风,吴丽云,李慧.红曲生产菌种选择管理中的问题与思考[J].中药材,2018,41(2):490-496. [2] YANG Y,LIU B,DU X,et al.Complete genome sequence and transcriptomics analyses reveal pigment biosynthesis and regulatory mechanisms in an industrial strain,Monascus purpureus YY-1[J].Scientific Reports,2015,5:8 331. [3] LIU Z,WANG Z,LYU X,et al.Comparison study of the volatile profiles and microbial communities of Wuyi Qu and Gutian Qu,two major types of traditional fermentation starters of Hong Qu glutinous rice wine[J].Food Microbiol,2018,69:105-115. [4] SHI Y C,PAN T M.Beneficial effects of Monascus purpureus NTU 568-fermented products:A review[J].Appl Microbiol Biotechnol,2011,90:1 207-1 217. [5] CHEN W,HE Y,ZHOU Y,et al.Edible filamentous fungi from the species Monascus:early traditional fermentations,modern molecular biology,and future genomics[J].Compr Rev Food Sci Food Saf,2015,14:555-567. [6] ENDO A.Monacolin K,a new hypocholesterolemic agent produced by a Monascus species[J].J Antibiot (Tokyo),1979,32:852-854. [7] BLANC P J,LAUSSAC J P,LE BARS J,et al.Characterization of monascidin A from Monascus as citrinin[J].Int J Food Microbiol,1995,27:201-213. [8] 李贞景,薛意斌,刘妍,等.红曲菌中桔霉素的控制策略及研究进展[J].食品科学,2018,39(17):263-268. [9] GB 1886.181—2016,食品安全国家标准食品添加剂红曲红[S].北京:中国标准出版社,2016. [10] GB 1886.66—2015,食品安全国家标准食品添加剂红曲黄色素[S].北京:中国标准出版社,2015. [11] QB/T 2847—2007,功能性红曲米(粉)[S].北京:中国轻工业出版社,2007. [12] 刘颖,林风,郑军荣,等.低桔霉素且产Monacolin K红曲菌株的筛选与鉴定[J].福建农林大学学报(自然科学版),2015,44(5):456-461. [13] FENG Y,CHEN W,CHEN F.A Monascus pilosus MS-1 strain with high-yield monacolin K but no citrinin[J].Food Sci Biotechnol,2016,25:1 115-1 122. [14] 姜冰洁,许赣荣,张薄博,等.降脂红曲产品质量标准的探讨[J].中草药,2015,46(3):453-456. [15] 李志强,刘颖,林风,等.福建古田红曲生产用红曲霉菌主要种类的鉴别[J].食品与发酵工业,2017,43(5):64-69. [16] SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J].Proc Natl Acad Sci USA,2012,109:6 241-6 246. [17] TAMURA K,STECHER G,PETERSON D,et al.MEGA 6:Molecular evolutionary genetics analysis version 6.0[J].Mol Biol Evol,2013,30:2 725-2 729. [18] BARBOSA R N,LEONG S L,VINNERE-PETTERSSON O,et al.Phylogenetic analysis of Monascus and new species from honey,pollen and nests of stingless bees[J].Stud Mycol,2017,86:29-51. [19] GB 5009.222—2016,食品安全国家标准食品中桔青霉素的测定[S].北京:中国标准出版社,2016. [20] 李钟庆,郭芳.红曲菌的形态与分类学[M].北京:中国轻工业出版社,2003. [21] SHAO Y,LEI M,MAO Z,et al.Insights into Monascus biology at the genetic level[J].Appl Microbiol Biotechnol,2014,98:3 911-3 922. [22] SHAO Y,XU L,CHEN F.Genetic diversity analysis of Monascus strains using SRAP and ISSR markers[J].Mycoscience,2011,52:224-233. [23] PARK H G,JONG S C.Molecular characterization of Monascus strains based on the D1/D2 regions of LSU rRNAgenes[J].Mycoscience,2003,44:25-32. [24] PARK H G,ELENA K S,JONG S C.Phylogenetic relationships of Monascus species inferred from the ITS and the partial β-tubulin gene[J].Bot Bull Acad Sin,2004,45:325-330. [25] SHIMIZU T,KINOSHITA H,ISHIHARA S,et al.Polyketide synthase gene responsible for citrinin biosynthesis in Monascuspurpureus[J].Appl Environ Microb,2005,71:3 453-3 457. [26] SHIMIZU T,KINOSHITA H,NIHIRA T.Identification and in vivo functional analysis by gene disruption of ctnA,an activator gene involved in citrinin biosynthesis in Monascuspurpureus[J].Appl Environ Microb,2007,73:5 097-5 103. [27] CHEN Y P,TSENG C P,CHIEN I L,et al.Exploring the distribution of citrinin biosynthesis related genes among Monascusspecies[J].J Agric Food Chem,2008,56:11 767-11 772.