Abstract: In this study, polysaccharides from Matricaria chamomilla, extracted by reflux were comprehensively investigated. Two homogenous polysaccharides (MCP-1-1 and MCP-2-1) were obtained through DEAE-Sepharose and Sephadex G-100 chromatography. The total polysaccharide contents of MCP-1-1 and MCP-2-1 were 82.23% and 84.09% respectivley. Moreover, the contents of uronic acid were 4.11% and 5.38% respectively. And the protein contents were 0.58% and 1.05%. Besides, the solubility of MCP-1-1 and MCP-2-1 were 98.36% and 97.44%. Furthermore, MCP-1-1 was composed of rhamnose, glucuronic acid, galactose, glucose, xylose and arabinose, meanwhile MCP-2-1 was composed of glucuronic acid, galactose, glucose, galacturonic acid, xylose and arabinose. The molecular weight of MCP-1-1 and MCP-2-1were 30 902 and 7 244 Da. Further study indicated that two polysaccharides had pyran ring and β- type glycosidic linkages and had no triple- helix structure, showed excellent thermal stability. Two homogenous polysaccharides had scavenging effect on both DPPH and hydroxyl free radical, and the scavenging ability was positively correlated with the concentration of homogeneous polysaccharides, the scavenging ability of MCP-2-1 was significantly higher than that of MCP-1-1. The research results revealed that MCP-1-1 and MCP-2-1 could be used as natural antioxidant in food and cosmetics industry, and this study could provide theoretical support for the development and utilization of chamomile polysaccharides.
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2021, Vol. 47 
