The optimization of the extract condition, kinetics, and molecular characteristics of water-soluble polysaccharides from Pisum sativum L. seed pericarp
QU Lin, AI Lianzhong, LAI Phoency*, ZHANG Hui
(Shanghai Engineering Research Center of Food Microbiology,School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology,Shanghai 200093,China)
Abstract: The aim of this study was to investigate the optimum extract condition for water-soluble polysaccharides from Pisum sativum L. seed pericarp, elucidate extraction kinetic parameters, and identify the monosaccharide compositions and molecular characteristics of the polysaccharides. Seventeen extraction conditions designed by Box-Behnken experimental design were applied. After response surface statistical analysis, the optimal extraction condition was: liquid-material ratio 25 mL/g; extraction temperature 91℃ for 1.5 h. Under this condition, the maximal yield was 4.4%. The given multiple regression model could describe very well for yield variations in terms of extraction factors. The increase in polysaccharide yield with extraction time at 90℃ could be closely explained by two-stage first-order diffusion kinetics with the early-and late-stage extraction rate constant (k1) of 0.028 8 and 0.090 7 min-1, respectively. Alternatively, it could also be explained by one-stage pseudo-first-order kinetics (n=0.81) with an extraction rate constant kn=0.023 7 min-1. The polysaccharides were mainly composed of arabinose, xylose, and galacturonic acid (molar ratio=1∶0.55∶0.45), implying the presence of arabinans, xylans, and pectins. Moreover, for molecular characteristics, the polysaccharides showed a weight-averaged molecular weight (Mw) = 338.9 kDa; polydispersity index (PDI) = 3.67; mean-square radius of gyration (Rg) = 46.8 nm; hydrodynamic radius (Rh) = 33.8 nm; Rg/Rh = 1.38, intrinsic viscosity [η] = 85.8 mL/g, and the exponent α for Mark-Houwink-Sakurada relationship [η] = K×Mwα) = 0.372. These results suggested that the chain conformation of the polysaccharides adapted flexible random-coil sphere. Conclusively, water-soluble polysaccharides from Pisum sativum L. seed pericarp would be suitable for the production of valorized arabinans and xylans.
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QU Lin,AI Lianzhong,LAI Phoency,et al. The optimization of the extract condition, kinetics, and molecular characteristics of water-soluble polysaccharides from Pisum sativum L. seed pericarp[J]. Food and Fermentation Industries, 2020, 46(21): 81-89.
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2020, Vol. 46 
