Abstract: To analyze the internal molecular mechanism of Saccharomyces cerevisiae under selenium-enriched conditions from the omics level and provide a theoretical basis for the study of selenium-enriched S. cerevisiae and the mining and utilization of selenium-enriched genes, S. cerevisiae cultured without selenium was used as the control group Kb, and S. cerevisiae cultured with 20 μg/mL selenium was used as the experimental group Se. Illumina high-throughput sequencing platform was used to sequence the transcriptome. Data were analyzed and processed by a variety of bioinformatics methods. The results showed that a total of 6 445 Unigenes were obtained by transcriptome sequencing, including 1 401 (21.74%), 3 665 (56.87%), 5 630 (87.35%), 6 112 (94.83%), 6 077 (94.29%) and 5 059 (78.49%) Unigenes were annotated to GO, KEGG, COG, NR, Swiss Prot and Pfam databases, and a total of 6 150 (95.42%) Unigenes were annotated. In the GO functional annotation, a total of 41 GO functional subclasses were obtained, and 113 KEGG pathways were obtained in the KEGG metabolic pathway analysis. The high-quality transcriptome sequencing data and wide coverage provide a certain theoretical reference for selenium-enriched gene mining and research of S. cerevisiae.
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