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食品与发酵工业  2021, Vol. 47 Issue (1): 138-142    DOI: 10.13995/j.cnki.11-1802/ts.024873
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究
高雪婧, 罗思奕, 汤凯洁, 罗秋水*
(江西农业大学 食品学院,江西 南昌,330045)
Effect of crude hawthorn glycoprotein on hypolipidemic and antioxidant activity in high-fat mice
GAO Xuejing, LUO Siyi, TANG Kaijie, LUO Qiushui*
(School of Food Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China)
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摘要 采用水提醇沉法提取有效成分,Sevage法去除游离蛋白,DEAE-52 纤维素层析及SephadexG-75纯化制备山楂粗糖蛋白,并选择昆明(KM)小鼠为研究对象,随机分为空白对照组、高脂模型组、山楂粗糖蛋白低、中、高剂量组。8周后检测小鼠血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量;检测肝脏过氧化氢酶(catalase,CAT)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性,总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malonaldehyde,MDA)含量。结果表明,与空白对照组相比,高脂模型组的TC、TG、MDA水平升高,HDL-C、CAT、T-SOD、T-AOC降低,且均极显著差异(P<0.01);与高脂模型组相比,山楂粗糖蛋白组的TC、TG、MDA水平降低(P<0.05),HDL-C、CAT、T-SOD、T-AOC含量升高(P<0.05),量效关系显著,由此可知,山楂粗糖蛋白对高脂小鼠具有良好的降脂作用和抗氧化活性。
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高雪婧
罗思奕
汤凯洁
罗秋水
关键词:  山楂  粗糖蛋白  高脂小鼠  降脂  抗氧化    
Abstract: The hypolipidemic and antioxidant activity of crude glycoprotein from hawthorn in high-fat mice were investigated in the paper.The active ingredients were extracted by water extraction and alcohol precipitation, the free protein was removed by Sevage method, DEAE-52 cellulose chromatography and SephadexG-75 purification were used to prepare crude hawthorn glycoprotein.KM mice were selected as the research objects, and randomly divided into blank control group, high-fat model group, hawthorn glycoprotein low, medium and high dose groups.After 8 weeks of gavage, the content of total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) in serum were measured, the activities of catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and the content of malonaldehyde (MDA) in liver were measured.Results showed that compared with the blank control group, the levels of TC, TG and MDA in the high-fat model group increased, while HDL-C, CAT, T-SOD and T-AOC decreased, all the differences were significant (P<0.01).Compared with the high-fat model group, the content of TC, TG and MDA in the hawthorn glycoprotein group decreased (P<0.05), and the content of HDL-C and the activities of CAT, T-SOD and T-AOC increased (P<0.05).The dose-effect relationship was significant, from this we can find that the crude glycoprotein from hawthorn had a better effect on hypolipidemic and antioxidant activity in high-fat mice.
Key words:  hawthorn    crude glycoprotein    high-fat mice    hypolipidemic    antioxidant activity
收稿日期:  2020-06-28      修回日期:  2020-07-23                发布日期:  2021-02-03      期的出版日期:  2021-01-15
基金资助: 国家自然科学基金项目(31760488);江西农业大学大学生创新创业训练项目(202010410231)
作者简介:  硕士研究生(罗秋水实验师为通讯作者,E-mail:lqsh2001@163.com)
引用本文:    
高雪婧,罗思奕,汤凯洁,等. 山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究[J]. 食品与发酵工业, 2021, 47(1): 138-142.
GAO Xuejing,LUO Siyi,TANG Kaijie,et al. Effect of crude hawthorn glycoprotein on hypolipidemic and antioxidant activity in high-fat mice[J]. Food and Fermentation Industries, 2021, 47(1): 138-142.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.024873  或          http://sf1970.cnif.cn/CN/Y2021/V47/I1/138
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