Abstract: Given that microbial fermentation is a complicated and dynamic process, it is difficult to regulate the metabolic flux due to the changing conditions in cellular growth. Furthermore, the fermentation system is a mixture which contains nongenetic variants with different production performance as well as different growth states. The current focus of fermentation engineering and metabolic engineering is how to simultaneously achieve the optimum growth and production states of individual cell in the fluctuating intra- and extra-cellular environment by dynamically regulating the metabolic network. According to the spatiotemporal distribution properties of metabolic flux perturbance factors, this review highlights the physical and chemical environment-based dynamic regulation strategies, and intracellular or extracellular metabolites-based dynamic regulation strategies. Theoretical and technical references for precise controlling of the fermentation process were also summarized.
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2020, Vol. 46 
