Abstract: The solid waste produced in brewing process,including sludge and distilled grains,is about three times of liquor.To investigate the conversion of the solid wastes discharged from Luzhou Laojiao Co.,Ltd.into an organic fertilizer by thermophilic composting,sorghum was supplied with the organic fertilizer to evaluate the fertilizer efficiency.The results showed that the mixture of sludge,distilled grains and decomposable inoculant was subject to heating,high temperature and cooling stages in the composting process.Concomitantly,nitrogen,phosphorus,potassium and humic acid in the compost substrates increased with moisture decreased while the substrate color changed from tawny to brown,black-brown or black.The mixture of sludge and distilled grains at the rate of 1∶2 in weight contained 65% moisture with about 25∶1 of C∶N ratio and pH 6.5 composted most rapidly.The organic fertilizer produced had high quality and fitted the National Organic Fertilizer Quality Standard (NY525-2012).The application of this organic fertilizer (OF) in combination with chemical fertilizers (CF) at the proportion of 1∶1 in the terms of nitrogen increased sorghum yield by 12.55%,grain nitrogen content by 36.70%,plant phosphorus uptake by 22.22% and potassium uptake by 29.20%,respectively,compared with those of CF alone.Besides,there were higher available nitrogen and enzyme activities (including phosphatase,urease and dehydrogenase) in the soil supplied with OF+CF than those of sole CF.Taken together,the mixture of sludge and distilled grains at an adequate proportion converted into an organic fertilizer through thermophilic composting,which fitted the National Standard.The application of OF plus CF at a proper rate increased sorghum yield,improved plant nutrient,and stimulated soil enzyme activity.
李哲,张宿义,杨红军,等. 利用酿酒废弃物生产有机肥及肥效研究[J]. 食品与发酵工业, 2021, 47(2): 160-166.
LI Zhe,ZHANG Suyi,YANG Hongjun,et al. Conversion of brewing wastes into an organic fertilizer and its fertilizer efficiency[J]. Food and Fermentation Industries, 2021, 47(2): 160-166.
[1] 张肖克.中小白酒企业发展战略思考[J].酿酒科技,2009(7):119-121;124. ZHANG X K.Small and medium-sized liquor-making enterprise development strategic thinking[J].Liquor-making Science &Technology,2009(7):119-121;124. [2] 陈飞,张良,霍丹群,等.浓香型白酒基酒的现状和发展趋势[J].中国酿造,2017,36(10):5-8. CHEN F,ZHANG L,HOU D Q,et al.Present situation and development trend of base liquor of Luzhou-flavor Baijiu[J].China Brewing,2017,36(10):5-8. [3] 李觅,刘念,王超凯,等.固态酿造废弃物规模化利用研究[J].酿酒科技,2018(2):113-116. LI M,LIU N,WANG C K,et al.Large-scale use of spent grains and waste water in distilleries[J].Liquor-making Science &Technology,2018(2):113-116. [4] 四川省宜宾五粮液集团有限公司.浓香型白酒丢糟综合利用方法:中国,200910303303.X[P].2009-10-28. Wuliangye Group Co.Ltd.,Yibin,Sichuan.Comprehensive utilization of distiller′s grains of Luzhou flavor liquor:China,200910303303.X[P].2009-10-28. [5] 刘跃红,吴正云,杨健,等.白酒丢糟的多酶复配降解制备可发酵性糖[J].生物加工过程,2014(6):18-22. LIU Y H,WU Z Y,YANG J,et al.Preparing of fermentable sugars from pretreatment Chinese liquor distillers′grains by using multi-enzyme hydrolysis[J].Chinese Journal of Bioprocess Engineering,2014(6):18-22. [6] 武汉森泰环保工程有限公司.一种酿酒废水处理污泥制备水处理生物活性促进剂的方法:中国,201310589023.6[P].2014-02-05. Wuhan Sentai Environmental Protection Engineering Co.,Ltd.A method for preparation of water treatment biological activity promoter from brewery wastewater treatment sludge:China,201310589023.6[P].2014-02-05. [7] 李文洪.白酒废水污泥特征及资源化利用研究[D].雅安:四川农业大学,2013. LI W H.Research on the properties and resources utilization of liquor sludge[D].Ya′an:Sichuan Agricultural University,2013. [8] 顾文杰,张发宝,徐培智,等.接种菌剂对堆肥微生物数量和酶活性的影响[J].农业环境科学学报,2009,28(8):168-172. GU W J,ZHANG F B,XU P Z,et al.Inoculum additions during composting:Impacts on microbial populations and enzyme activity[J].Journal of Agro-Environment Science,2009,28(8):168-172. [9] 张永锋,滕星,李忠和,等.玉米秸秆堆肥及其影响因素研究进展[J].吉林农业大学学报,2016,38(5):613-618. ZHANG Y F,TENG X,LI Z H,et al.Research progress of corn stalk compost and its influencing factors[J].Journal of Jilin Agricultural University,2016,38(5):613-618. [10] 劳德坤,张陇利,李永斌,等.不同接种量的微生物秸秆腐熟剂对蔬菜副产物堆肥效果的影响[J].环境工程学报,2015,9(6):451-457. LAO D K,ZHANG L L,LI Y B,et al.Effect of different inoculation amounts of microbial straw decomposition agents on vegetable byproducts composting[J].Chinese Journal of Environmental Engineering,2015,9(6):451-457. [11] HU H W,LI L M,QIAN K,et al.Effect of doses of fermentation inoculum on physico-chemical properties and related enzymic activities during the composting process[J].Journal of Agro-Environment Science,2013,32(6):1 261-1 270. [12] MOHAMMAD N,ALAM M Z,KABASHI N A.Optimization of effective composting process of oil palm industrial waste by lignocellulolytic fungi[J].Journal of Material Cycles and Waste Management,2015,17(1):91-98. [13] 中华人民共和国农业部.NY 525—2012 有机肥料[S].北京:中国农业出版社,2012. Ministry of Agriculture of the People′s Republic of China.NY 525—2012 Organic fertilizer[S].Beijing:China Agricultural Press,2012. [14] 中华人民共和国国家质量监督检验检疫局.GB/T 11957—2001煤中腐植酸产率测定方法[S].北京:中国标准出版社,2001. State Administration of Quality Supervision.GB/T 11957—2001 Determination of yield of humic acids in coal[S].Beijing:China Standards Press,2001. [15] 杨剑虹.土壤农化分析与环境监测[M].北京:中国大地出版社,2008. YANG J H.Soil agrochemical analysis and environmental monitoring[M].Beinjing:China Land Press,2008. [16] 关松荫.土壤酶及其研究法[M].北京:农业出版社,1986. GUAN S Y.Soil enzyme and its research methods[M].Beijing:Agricultural Press,1986. [17] 陈亚楠,张长华,梁永江,等.玉米秸秆堆肥的田间积制和原位还田肥效[J].应用生态学报,2014,25(12):130-136. CHEN Y N,ZHANG C H,LIANG Y J,et al.Corn straw composting in the field and in situ fertilizer effect[J].Chinese Journal of Applied Ecology,2014,25(12):130-136. [18] 姚伟卿.堆肥过程的生物学途径及主要影响因素的控制[J].四川环境,2008,27(5):93-98. YAO W Q.Biological Approach and control of main factors during the process of composting[J].Sichuan Environment,2008,27(5):93-98. [19] 杨红军,杜如万,吴叶宽,等.紫茎泽兰野外微生物堆肥及毒性评价[J].草业学报,2017,26(6):131-138. YANG H J,DU R W,WU Y K,et al.Microbial composting and detoxification of Ageratina adenophora[J].Acta Prataculturae Sinica,2017,26(6):131-138. [20] 赵建,袁玲,黄建国,等.柑橘皮渣高温堆肥生产有机肥[J].农业工程学报,2011,27(10):270-276. ZHAO J,YUAN L,HUANG J G,et al.High temperature compost of citrus pill residues to produce organic fertilizer[J].Transactions of the CSAE,2011,27(10):270-276. [21] 先元华.屠宰场污泥堆肥效果及其影响因素实验研究[J].环境工程,2015,33(1):112-116. XIAN Y H.Experimental study on the performance of slaughterhouse sludge compost and its influencing factors[J].Environmental Engineering,2015,33(1):112-116. [22] 张陇利,刘青,徐智,等.复合微生物菌剂对污泥堆肥的作用效果研究[J].环境工程学报,2008,2(2):266-269. ZHANG L L,LIU Q,XU Z,et al.Research on effect of compound microbial inoculant on sewage sludge composting[J].Chinese Journal of Environmental Engineering,2008,2(2):266-269. [23] 党秋玲,李鸣晓,席北斗,等.堆肥过程多阶段强化接种对细菌群落多样性的影响[J].环境科学,2011,32(9):195-201. DANG Q L,LI M X,XI B D,et al.Effects of multi-stage strengthening inoculation on bacterial community diversity during composting[J].Chinese Journal of Environmental Science,2011,32(9):195-201. [24] 龚昆,张莉,熊海容.秸秆堆肥降解过程中的微生物变化[J].湖北农业科学,2019,58(21):81-86. GONG K,ZHANG L,XIONG H R.Changes of microorganisms in the process of straw compost degradation[J].Hubei Agricultural Sciences,2019,58(21):81-86. [25] JERIS J S,REGAN R W.Controlling environmental parameters for optimal composting,PartⅢ[J].Compost Sci.,1973,l4(3):16-22. [26] MILLER F C.Matric water potential as an ecological determinant in compost,a substrate dense system[J].Microbial Ecology,1989,18(1):59-71. [27] 勾长龙,高云航,刘淑霞,等.微生物菌剂对堆肥发酵影响的研究进展[J].湖北农业科学,2013,52(6):1 244-1 247. GOU C L,GAO Y H,LIU S X,et al.Investigation and application progress of inoculating microbes on aerobic composting of manure[J].Hubei Agricultural Sciences,2013,52(6):1 244-1 247. [28] SMARS S,GUSTAFSSON L,BECK-FRIIS B,et al.Improvement of the composting time for household waste during an initial low pH phase by mesophilic temperature control[J].Bioresource Technology,2002,84(3):237-241. [29] 孙先锋,钟海风,谢式云,等.高效堆肥菌种的筛选及在城市污泥堆肥上的应用[J].环境污染治理技术与设备,2006,7(2):108-111. SUN X F,ZHONG H F,XIE S Y,et al.Filtration of high effect composting strains and its application in municipal sludge composting[J].Techniques and Equipment for Environmental Pollution Control,2006,7(2):108-111. [30] 曹云,黄红英,吴华山,等.畜禽粪便超高温堆肥产物理化性质及其对小白菜生长的影响[J].农业工程学报,2018,34(12):251-257. CAO Y,HUANG H Y,WU H S,et al.Physico-chemical properties of hyperthermophilic composting from livestock manures and its effects on growth of Chinese cabbage[J].Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE),2018,34(12):251-257. [31] OLAETXEA M,DE HITA D,GARCIA C,et al.Hypothetical framework integrating the main mechanisms involved in the promoting action of rhizospheric humic substances on plant root-and shoot-growth[J].Applied Soil Ecology,2018,123:521-537. [32] 唐玉明.高粱籽粒的酿酒品质研究[J].酿酒,2000(4):30-32. TANG Y M,Study on liquor-making quality of sorghum seed[J].Liquor Making,2000(4):30-32. [33] 秦华,林先贵,陈瑞蕊,等.DEHP对土壤脱氢酶活性及微生物功能多样性的影响[J].土壤学报,2005,42(5):829-834. QIN H,LIN X G,CHEN R R,et al.Effect of DEHP on dehydrogenase activity and microbial functional diversity in soil[J].Acta Pedologica Sinica,2005,42(5):829-834. [34] 陈鸿飞,庞晓敏,张仁,等.不同水肥运筹对再生季稻根际土壤酶活性及微生物功能多样性的影响[J].作物学报,2017,43(10):1 507-1 517. CHEN H F,PANG X M,ZHANG R,et al.Effects of different irrigation and fertilizer application regimes on soil rn-zyme activities and microbial functional diversity in rhizosphere of ratoon-ing rice[J].Acta Agronomica Sinica,2017,43(10):1 507-1 517.