Abstract: The effect of ethanol extract of onion skins (EEOS) on color browning, lipid and protein oxidative as well as infection degree of microorganisms of fresh pork was studied, and was compared with control (without any antioxidant) and ascorbic acid (VC). The objective was to determine the effect of color browning by brightness (L*), redness (a*), and the content of methemoglobin (MetMb). The inhibitory effect of EEOS on the oxidation of pork lipid and protein was studied based on thiobarbituric acid reacting substances (TBARS), hydrophobicity and carbonyl content of protein in samples at different time points. Total volatile basic nitrogen (TVB-N) content was used to characterize the infection degree of microorganisms to meat quality. Results showed that EEOS could reduce MetMb content and increase a* value of meat samples. On the 12th day, TBARS and carbonyl content of 2.0% EEOS treatment were decreased by 53.1% and 29.2% respectively than control, which were lower than those in VC treatments. In addition, TVB-N values and protein hydrophobicity of each EEOS treatment were lower than control, and showed a concentration dependent relationship (P<0.05). These results indicated that EEOS has a good research prospect as a natural antioxidant or preservative.
王存堂,高增明,张福娟,等. 洋葱皮乙醇提取物对生鲜猪肉色泽、脂质和蛋白质氧化稳定性的影响[J]. 食品与发酵工业, 2021, 47(3): 87-94.
WANG Cuntang,GAO Zengming,ZHANG Fujuan,et al. Effect of ethanol extract from onion skin on color, lipid and protein oxidative stability of fresh pork[J]. Food and Fermentation Industries, 2021, 47(3): 87-94.
[1] ZAHID M A, SEO J K, RASHIDA P, et al.Comparison of butylated hydroxytoluene, ascorbic acid, and clove extract as antioxidants in fresh beef patties at refrigerated storage[J].Food Science of Animal Resources,2019,39(5):768-779. [2] KARAKAYA M, BAYRAK E, ULUSOY K, et al.Use of natural antioxidants in meat and meat products[J].Journal of Food Science and Engineering,2011,1(1):1-10. [3] RODRÍGUEZ-CARPENA J G, MORCUENDE D, ESTÉVEZ M.Avocado by-products as inhibitors of color deterioration and lipid and protein oxidation in raw porcine patties subjected to chilled storage[J].Meat Science,2011,89(2):166-173. [4] CHENG J R, XU L, XIANG R, et al.Effects of mulberry polyphenols on oxidation stability of sarcoplasmic and myofibrillar proteins in dried minced pork slices during processing and storage[J].Meat Science,2020,160(C):107 973. [5] TRAN T T T, TON N M N, NGUYEN T T, et al.Application of natural antioxidant extract from guava leaves (Psidium guajava L.) in fresh pork sausage[J].Meat Science,2020,165:108 106. [6] DAS ARUN K, RAJKUMAR V, NANDA P K, et al.Antioxidant efficacy of litchi (Litchi chinensis Sonn.) pericarp extract in sheep meat nuggets[J].Antioxidants (Basel, Switzerland),2016,5(2):1-10. [7] 刘世馨. 洋葱皮多酚、黄酮提取及其体外抗氧化等活性研究[D].北京:中国农业科学院,2018. LIU S X.Study on extraction and antioxidant activity in vitro and others of polyphenols, flavonoids from onion skin[D].Beijing:Chinese Academy of Agricultural Sciences,2018. [8] LEE K A,KIM K T,NAH S Y,et al.Antimicrobial and antioxidative effects of onion peel extracted by the subcritical water[J].Food Science and Biotechnology,2011,20(2):543-548. [9] LEE S Y,KIM H W,HWANG K E,et al.Combined effect of kimchi powder and onion peel extract on quality characteristics of emulsion sausages prepared with irradiated pork[J].Korean Journal for Food Science of Animal Resources,2015,35(3):277-285. [10] 任曼妮, 高增明,王存堂.不同溶剂提取对洋葱皮中多酚含量及抗氧化活性的影响[J].食品与发酵工业,2019,45(17):189-193. REN M N,GAO Z M,WANG C T.Effects of extraction solvents on the phenolic contents and antioxidant activities of onion (Allium cepa L.) peels[J].Food and Fermentation Industries,2019,45(17):189-193. [11] FRIEDRICH L,SIRÓ I,DALMADI I,et al.Influence of various preservatives on the quality of minced beef under modified atmosphere at chilled storage[J].Meat Science,2007,79(2):332-343. [12] 何晓燕, 蒋其斌,夏天兰,等.不同浓度茶多酚对冷却肉的影响分析[J].食品工业科技,2010,31(6):81-83. HE X Y,JIANG Q B,XIA T L,et al.Effects of tea polyphenols with different concentration on chilled pork[J].Science and Technology of Food Industry,2010,31(6):81-83. [13] ZENGIN H,BAYASAL H A.Antioxidant and antimicrobial activities of thyme and clove essential oils and application in minced beef[J].Journal of Food Processing and Preservation,2015,39(6):1 261-1 271. [14] BASIRI S,SHEKARFOROUSH S S,AMINLARI M,et al.The effect of pomegranate skin extract (PPE) on the polyphenol oxidase (PPO) and quality of pacific white shrimp (Litopenaeus vannamei) during refrigerated storage[J].LWT-Food Science and Technology,2015,60(2):1 025-1 033. [15] 贾娜, 王乐田,邵俊花,等.芦丁对冷藏猪肉糜脂肪和蛋白氧化及品质特性的影响[J].食品与发酵工业,2016,42(10):105-111. JIA N,WANG L T,SHAO J H,et al.Effect of rutin on lipid/protein oxidation and quality of minced pork during chilled storage[J].Food and Fermentation Industries,2016,42(10):105-111. [16] 梁慧, 于立梅,陈秀兰,等.多酚对鸡肉氧化脂肪诱导蛋白质变性的影响[J].食品与发酵工业,2016,42(5):146-151. LIANG H,YU L M,CHEN X L,et al.Effect of garcinia mangostana polyphenols on protein degeneration induced by chicken oxidation fat[J].Food and Fermentation Industries,2016,42(5):146-151. [17] JEONG C H,HEO H J,CHOI S G,et al.Antioxidant and anticancer properties of methanolic extracts from different parts of white,yellow,and red onion[J].Food Science and Biotechnology,2009,18:108-112. [18] HAJRAWATI,NURAINI H,ARIEF I I,et al.Lipid oxidation and antimicrobial activity of cooked beef patties as influenced by leaf extracts of “Cemba” (Albizia lebbeckoides[DC.] Benth)[J].Bulletin of Animal Science,2019,43(1):38-45. [19] DE OLIVEIRA FERRERA N S,ROSSET M,LINA G,et al.Effect of adding Brosimum gaudichaudii and Pyrostegia venusta hydroalcoholic extracts on the oxidative stability of beef burgers[J].LWT-Food Science and Technology,2019,108:145-152. [20] LEE M A,KIM T K,HWANG K E,et al.Kimchi extracts as inhibitors of colour deterioration and lipid oxidation in raw ground pork meat during refrigerated storage[J].Journal of the Science of Food and Agriculture,2019,99(6):2 735-2 742. [21] 肖朝耿, 谭芦兰,朱培培,等.红叶甜菜在肉糜中的发色效果及其抗氧化作用[J].中国食品学报,2019,19(5):111-121. XIAO C G,TAN L L,ZHU P P,et al.The coloring effect and antioxidant effect of red beet in minced pork[J].Journal of Chinese Institute of Food Science and Technology,2019,19(5):111-121. [22] FERNANDES R P P,TRINDADE M A,TONIN F G,et al.Evaluation of oxidative stability of lamb burger with,Origanum vulgare,extract[J].Food Chemistry,2017,233:101-109. [23] 王玮, 汤祥明,金邦荃.高铁肌红蛋白含量和高铁肌红蛋白还原酶活性与冷鲜肉肉色稳定性关系的研究[J].食品科学,2008(7):94-97. WANG W,TANG X M,JIN B Q.Study on correlation of metmyoglobin content and metmyoglobin reductase activity with color stability of chilled pork[J].Food Science,2008(7):94-97. [24] SADEGHINEJAD N,SARTESHNIZI R.A,GAVLIGHI H A,et al.Pistachio green hull extract as a natural antioxidant in beef patties:Effect on lipid and protein oxidation,color deterioration,and microbial stability during chilled storage[J].LWT-Food Science and Technology,2018,102:393-402. [25] KANATT S R,CHANDER R,RADHAKRISHNA P,et al.Potato peel extract natural antioxidant for retarding lipid peroxidation in radiation processed lamb meat[J].Journal of Agricultural and Food Chemistry,2005,53(5):1 499-1 504. [26] TURGUT S S,SOYER A,ISIKCI F.Effect of pomegranate skin extract on lipid and protein oxidation in beef meatballs during refrigerated storage[J].Meat Science,2016,116:126-132. [27] KIM W J,LEE K A,KIM K T,et al.Antimicrobial effects of onion (Allium cepa L.) peel extracts produced via subcritical water extraction against Bacillus cereus strains as compared with ethanolic and hot water extraction[J].The Korean Society of Food Science and Technology,2011,20(4):1 101-1 106. [28] SANTAS J,ALMAJANO M P,CARBÓR.Antimicrobial and antioxidant activity of crude onion (Allium cepa,L.) extracts[J].International Journal of Food Science & Technology,2010,45(2):403-409. [29] CHELH I,GATELLIER P,SANTÉ-LHOUTELLIER V.Technical note:A simplified procedure for myofibril hydrophobicity determination[J].Meat science,2006,74(4):681-683. [30] 曹云刚.植物多酚对肉蛋白氧化稳定性和功能特性的影响机理及应用[D].无锡:江南大学,2016. CAO Y G.Effect of plant-derived polyphenols on oxidative stability and functional properties of meat proteins:mechanism and application[D].Wuxi:Jiangnan University,2016.
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