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食品与发酵工业  2021, Vol. 47 Issue (2): 102-108    DOI: 10.13995/j.cnki.11-1802/ts.024997
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
焦谷氨酸对高盐饮食小鼠肠道健康及肠道菌群的作用
徐珒昭1, 汤梦琪1, 徐境含1, 滕国新2, 许晓曦1*
1(东北农业大学 食品学院,黑龙江 哈尔滨,150030)
2(内蒙古蒙牛乳业(集团)股份有限公司,内蒙古 呼和浩特,011500)
Effects of pyroglutamic acid on intestinal health and intestinal microflora in mice with high-salt diet
XU Jinzhao1, TANG Mengqi1, XU Jinghan1, TENG Guoxin2, XU Xiaoxi1*
1(College of Food Science,Northeast Agricultural University,Harbin 150030,China)
2(Inner Mongolia Meng Niu Dairy Industry(Group)Co.,Ltd.,Huhhot 011500,China)
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摘要 建立高盐饮食小鼠模型,给予小鼠焦谷氨酸干预,通过对小鼠小肠病理学分析和形态学观察,检测炎性细胞因子、粪便中白蛋白渗漏量等相关物质,结合高通量测序分析小鼠盲肠内容物中菌群的α-多样性、β-多样性及基于门、属水平的物种组成,探究焦谷氨酸对高盐饮食小鼠肠道健康和肠道菌群的作用,开发其新应用方向。结果表明,焦谷氨酸可以显著缓解由高盐饮食引起的小鼠肠道结构损伤、降低小肠炎症细胞因子分泌水平。同时,焦谷氨酸可减少部分由高盐饮食导致显著增加的菌群丰度,例如拟杆菌属(Bacteroides)、另枝菌属(Alistipes)等;另使部分在高盐饮食过程中减少的菌群丰度得到恢复和部分益生菌丰度增加,包括分节丝状菌(Candidatus Arthromitus)、双歧杆菌属(Bifidobacterium)和乳酸杆菌属(Lactobacillus)等多种益生菌。综上,焦谷氨酸对高盐饮食小鼠机体显著的有益作用,可以稳定肠道结构、降低肠道炎症水平和改善肠道菌群结构和丰度。
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徐珒昭
汤梦琪
徐境含
滕国新
许晓曦
关键词:  焦谷氨酸  高盐饮食  肠道结构  肠道炎症  肠道菌群    
Abstract: In this experiment,a high-salt diet model was established in mice.The model mice were given pyroglutamic acid intervention to explore the effect of pyroglutamic acid on intestinal health and intestinal flora in mice with high-salt diet.Pathological analysis and morphological observation were performed on the small intestine of mice,followed by detection of intestinal inflammatory cytokines and albumin leakage in feces.Alpha diversity,beta diversity,and intestinal flora composition at the phylum and genus levels were analyzed by high-throughput sequencing.The results showed that pyroglutamic acid significantly alleviated the intestinal structural damage of mice caused by high-salt diet and reduced the secretion of inflammatory cytokines in small intestine.While pyroglutamic acid inhibited the significant increase in the abundance of some flora caused by high-salt diet,such as Bacteroides,Alistipes and other microorganisms.In addition,pyroglutamic acid also restored and increased the abundance of flora that had been reduced during high-salt diet,including Candidatus Arthromitus,Bifidobacterium,Lactobacillus and other probiotics.In summary,pyroglutamic acid has a significant beneficial effect on high-salt diet mice,which can stabilize the intestinal structure,reduce the level of intestinal inflammation,and improve the intestinal flora structure and abundance.
Key words:  pyroglutamic acid    high-salt diet    gut structure    gut inflammation    intestinal microflora
收稿日期:  2020-07-08      修回日期:  2020-09-06           出版日期:  2021-01-25      发布日期:  2021-02-07      期的出版日期:  2021-01-25
基金资助: 内蒙古自治区科技成果转化项目(CGZH2018035)
作者简介:  硕士研究生(许晓曦教授为通讯作者,E-mail:xiaoxi_xu01@163.com)
引用本文:    
徐珒昭,汤梦琪,徐境含,等. 焦谷氨酸对高盐饮食小鼠肠道健康及肠道菌群的作用[J]. 食品与发酵工业, 2021, 47(2): 102-108.
XU Jinzhao,TANG Mengqi,XU Jinghan,et al. Effects of pyroglutamic acid on intestinal health and intestinal microflora in mice with high-salt diet[J]. Food and Fermentation Industries, 2021, 47(2): 102-108.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.024997  或          http://sf1970.cnif.cn/CN/Y2021/V47/I2/102
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