Please wait a minute...
 
 
食品与发酵工业  2021, Vol. 47 Issue (4): 247-252    DOI: 10.13995/j.cnki.11-1802/ts.025009
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
麦麸酯酶抑制法检测辛辣蔬菜假阳性消除的研究
姜露1,2, 叶麟2,3, 侯晓艳2, 申光辉2, 黎杉珊2, 张志清2*
1(濮阳市食品药品检验检测中心,河南 濮阳,457000)
2(四川农业大学 食品学院,四川 雅安,625014)
3(四川国检检测有限责任公司,四川 泸州,646000)
Elimination of false positive in the detection of spicy vegetables by wheat bran esterase-inhibition method
JIANG Lu1,2, YE Lin2,3, HOU Xiaoyan2, SHEN Guanghui2, LI Shanshan2, ZHANG Zhiqing2*
1(Puyang Center for Food and Drug Inspection,Puyang 457000,China)
2(College of Food Science, Sichuan Agricultural University,Ya'an 625014,China)
3(Sichuan Guojian Testing Co.,Ltd,Luzhou 646000,China)
下载:  HTML   PDF (2972KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 利用酶抑制法可以快速检测蔬菜中的有机磷和氨基甲酸酯类农药残留,这两者能够抑制酯酶的活性,而辛辣蔬菜中含有影响酶活性的含硫化合物,容易导致检测结果出现假阳性。为了消除辛辣蔬菜造成的假阳性,以酶活性抑制率为指标,采用不同的加热方式、时间对4种辛辣蔬菜进行前处理。结果表明,对样品提取液使用水浴加热可起到消除假阳性的作用,其中香葱、洋葱、大蒜和青椒的最佳水浴加热温度分别为80、90、80、80 ℃,经过处理后的样品提取液对麦麸酯酶的抑制率分别由原来的37.06%、9.65%、17.02%、50.34%下降到12.69%、2.95%、5.79%、33.61%。除青椒外,该方法可在一定程度上消除或大大降低植物酯酶抑制法中假阳性的影响,且不会造成农药残留的损失,从而提高酶抑制法对辛辣蔬菜快速检测的准确性。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
姜露
叶麟
侯晓艳
申光辉
黎杉珊
张志清
关键词:  麦麸酯酶  酶抑制法  有机磷  氨基甲酸酯  辛辣蔬菜  假阳性    
Abstract: The enzyme inhibition method can be used to quickly detect organophosphate and carbamate pesticide residues, which inhibit the activity of esterase. But spicy vegetables also contain sulfur compounds that affect enzyme activity, which can lead to false positives. In this experiment, using activity inhibition rate as the index, different heating treatment and time to deal with 4 kinds of spicy vegetables were carried out to eliminate or reduce the false positive in enzyme inhibition method. The results indicated that under the appropriate heating temperature, which was 80 ℃, 90 ℃, 80 ℃, 80 ℃, for chives, onion, garlic and green pepper, respectively, the inhibition rate of enzyme activity decreased from 37.06%, 9.65%, 17.02%, 50.34% to 12.69%, 2.95%, 5.79%, 33.61%, respectively. In addition to green pepper, to some extent, this method could eliminate or greatly reduce the false positive that brought by spicy vegetables in the enzyme-inhibition method, which could improve the accuracy in enzyme-inhibition method to detect organophosphate and carbamate pesticide residues in spicy vegetables.
Key words:  wheat bran esterase    enzyme-inhibition methods    organophosphorus    carbamate    spicy vegetables    false positive
收稿日期:  2020-07-09      修回日期:  2020-09-11           出版日期:  2021-02-25      发布日期:  2021-03-16      期的出版日期:  2021-02-25
作者简介:  硕士研究生,助理工程师(张志清教授为通讯作者,E-mail:zqzhang721@163.com)
引用本文:    
姜露,叶麟,侯晓艳,等. 麦麸酯酶抑制法检测辛辣蔬菜假阳性消除的研究[J]. 食品与发酵工业, 2021, 47(4): 247-252.
JIANG Lu,YE Lin,HOU Xiaoyan,et al. Elimination of false positive in the detection of spicy vegetables by wheat bran esterase-inhibition method[J]. Food and Fermentation Industries, 2021, 47(4): 247-252.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025009  或          http://sf1970.cnif.cn/CN/Y2021/V47/I4/247
[1] 祝文峰, 李太平.基于文献数据的我国蔬菜农药残留现状研究[J].经济问题, 2018(11):92-98.
ZHU W F, LI T P.Study on the status of vegetable pesticide residues in china based on literature data[J].On Economic Problems, 2018(11):92-98.
[2] 任晓姣, 白亚迪, 王党党, 等.西安市蔬菜水果有机磷农药残留规律研究[J].安徽农业科学, 2017, 45(1):91-93.
REN X J, BAI Y D, WANG D D, et al.Study on organophosphorus pesticide residues in vegetables and fruits in Xi'an[J].Journal of Anhui Agricultural Sciences, 2017, 45(1):91-93.
[3] 罗月, 朱永义, 何远东.2015-2017年某市蔬菜和水果中农药残留现状分析[J].医学动物防制, 2019, 35(7):651-653.
LUO Y, ZHU Y Y,HE Y D.Analysis of pesticide residues in vegetables and fruits in a city during 2015-2017[J].Journal of Medical Pest Control,2019, 35(7):651-653.
[4] LOZOWICKA B, ABZEITOVA E, SAGITOV A, et al.Studies of pesticide residues in tomatoes and cucumbers from Kazakhstan and the associated health risks[J].Environmental Monitoring & Assessment, 2015, 187(10):609.
[5] 郭子森, 刘永军, 沈娟.吉林省蔬菜农药残留现状及建议[J].吉林农业, 2018, 21(47):86-86.
GUO Z S, LIU Y J, SHEN J.Present situation and suggestion of pesticide residue in vegetables in Jilin province[J].Agriculture of Jilin, 2018, 21(47):86-86.
[6] 刘亚平, 张金艳, 孙东立.酶抑制率法在4种蔬菜农药残留快速检测中的应用[J].安徽农业科学,2014, 42(2):427-428;470.
LIU Y P, ZHANG J Y, SUN D L.Application of enzyme inhibition rate method in rapid detection of four vegetables pesticide residues[J].Journal of Anhui Agricultural Sciences,2014, 42(2):427-428;470.
[7] 胡贝贞, 宋伟华, 谢丽萍, 等.气相色谱-负化学离子源质谱法测定含硫蔬菜中残留的14种农药[J].色谱, 2008, 26(5):608-612.
HU B Z, SONG W H, XIE L P, et al.Determination of 14 pesticide residues in sulfur-containing vegetables by gas chromatography-negative chemical ionization mass spectrometry[J].Chinese Journal of Chromatography,2008, 26(5):608-612.
[8] 中华人民共和国卫生部, 中国国家标准化管理委员会, GB/T 5009.218—2008 水果和蔬菜中多种农药残留量的测定[S].北京:中国标准出版社,2008.
Ministry of Health of the People's Republic of China, Standardization Administration of China.GB/T 5009.218—2008 Determination of multi pesticide residues in fruits and vegetables[S].Beijing:Standards Press of China, 2008.
[9] 陈龙军, 郭得平.葱蒜类作物中的蒜氨酸酶[J].植物生理学通讯, 2001, 37(5):471-474.
CHEN L J, GUO D P.Alliinase in garlic and allied species[J].Plant Physiology Communications, 2001, 37(5):471-474.
[10] 洪萍, 应杏秋.含硫蔬菜农药残留检测方法研究进展[J].中国卫生检验杂志, 2011, 21(9):2 355-2 357.
HONG P, YING X Q.Research progress on determination methods of pesticide residues in sulfur-containing vegetables[J].Chines Journal of Health Laboratory Technology, 2011, 21(9):2 355-2 357.
[11] 周华, 汪尤刚, 边天斌.磷酸处理-气相色谱法测定韭菜中有机磷农药[J].中国卫生检验杂志, 2012, 22(3):649-650.
ZHOU H, WANG Y G, BIAN T B.Determination of organophosphorus pesticides in leek by phosphoric acid treatment-gas chromatography[J].Chines Journal of Health Laboratory Technology,2012, 22(3):649-650.
[12] 郑金武, 江晓燕.蔬菜农药残留快速检测中消除假阳性的检测方法[J].安徽农学通报, 2012, 18(19):189;192.
ZHENG J W, JIANG X Y.Elimination of false positives in rapid detection of pesticide residues in vegetables[J].Anhui Agricultural Science Bulletin, 2012, 18(19):189;192.
[13] 侯晓艳, 姜露, 叶麟, 等.纯化方式对麦麸酯酶农药检测效能的影响[J].基因组学与应用生物学, 2019, 38(1):276-282.
HOU X Y, JIANG L, YE L, et al.Effect of purification methods on detection efficiency of wheat bran esterase[J].Genomics and Applied Biology,2019, 38(1):276-282.
[14] 侯晓艳, 姜露, 叶麟, 等.麦麸酯酶对有机磷及氨基甲酸酯类农药的动力学特性[J].分析测试学报, 2018, 37(3):324-330.
HOU X Y, JIANG L, YE L, et al.Kinetics characteristics of Wheat Bran esterase on organophosphorus and carbamate pesticides[J].Journal of Instrumental Analysis, 2018, 37(3):324-330.
[15] 叶麟, 杨雪, 张志清, 等.响应面法优化麦麸酯酶提取工艺[J].麦类作物学报, 2017, 37(3):420-427.
YE L, YANG X, ZHANG Z Q, et al.Optimization of extraction process for Wheat Bran esterase by response surface methodology[J].Journal of Triticeae Grops, 2017, 37(3):420-427.
[16] 中华人民共和国卫生部, 中国国家标准化管理委员会. GB/T 5009.199—2003 蔬菜中有机磷和氨基甲酸酯类农药残留量的快速检测[S].北京:中国标准出版社,2003.
Ministry of Health of the People's Republic of China, Standardization Administration of China.GB/T 5009.199—2003 Rapid detection for organophosphorus and carbamate pesticide residues in vegetables[S].Beijing:Standards Press of China, 2003.
[1] 曹栩菡, 黄小军, 叶麟, 潘才惠. QuEChERS-超滤净化结合GC-QQQ测定酿酒大曲中多种农药残留量[J]. 食品与发酵工业, 2021, 47(7): 238-243.
[2] 黄嫒, 马志强, 王玮, 宋燕飞, 李荣, 陈强, 陈祥贵, 杨潇. 豌豆酯酶的纯化和酯酶活性表征及其对农药的敏感性[J]. 食品与发酵工业, 2019, 45(11): 78-84.
[3] 安红梅 , 柯润辉 , 王丽娟 , 等. 固相萃取-气相色谱-火焰光度法同时测定植物性肽粉中42种有机磷农药残留[J]. 食品与发酵工业, 2018, 44(6): 245-250.
[4] 姜露,叶麟,杨雪,黎杉珊,申光辉,张志清. 不同前处理对植物酯酶抑制法检测蔬菜中有机磷及氨基甲酸酯类农药残留的影响[J]. 食品与发酵工业, 2016, 42(1): 200-.
[5] 沈丹玉, 刘毅华, 汤富彬, 钟冬莲, 莫润宏, 丁明,. MSPD-GC法检测竹笋中有机磷类农药残留[J]. 食品与发酵工业, 2013, 39(09): 185-188.
[6] 韩礼,侯亚西,汪俊涵,陈芳. 不同清洗方式对生菜表面农药残留的降解效果[J]. 食品与发酵工业, 2011, 37(12): 76-80.
[7] 周建科,徐鹏,杨东霞. 微萃取瓶富集-液相色谱法测定苹果醋中氨基甲酸酯类农药[J]. 食品与发酵工业, 2011, 37(06): 165-167.
[8] 蒋红英. 娃娃菜农残消降组合方法及对品质的影响[J]. 食品与发酵工业, 2011, 37(04): 92-96.
[9] 刘功良,陶嫦立,白卫东,赵文红. 有机磷农药的免疫检测研究进展[J]. 食品与发酵工业, 2010, 36(01): 113-117.
[10] 刘畅,陈福生,高海生,刘绍军. 鸭血清胆碱酯酶抑制法检测果蔬农药残留的研究[J]. 食品与发酵工业, 2009, 35(1): 157-.
[11] 张媛媛,赵广华,葛毅强,陈芳,胡小松. 食品中有机磷杀虫剂残留分析方法的研究进展[J]. 食品与发酵工业, 2007, 33(2): 114-.
[12] 闵建华,李建科,朱桂勤,何婷,牛乐. 电位法快速检测有机磷及氨基甲酸酯类农药灵敏度的研究[J]. 食品与发酵工业, 2006, 32(6): 111-.
[13] 曾令琴,陈芳,吴继红,廖小军,葛毅强,胡小松. 臭氧水降解苹果表面有机磷农药的研究[J]. 食品与发酵工业, 2006, 32(2): 16-.
[14] 李延华,张兰威,王伟君. 微生物法检测乳中抗生素残留及假阳性产生的原因[J]. 食品与发酵工业, 2005, 31(8): 93-.
[15] 梅文泉,黎其万,佴注. 牛乳中多种有机磷农药残留的双毛细管气相色谱测定法[J]. 食品与发酵工业, 2005, 31(2): 114-.
No Suggested Reading articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
版权所有 © 《食品与发酵工业》编辑部
地址:北京朝阳区酒仙桥中路24号院6号楼111室
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn