Abstract: Using citrus pectin as raw material, the process conditions for preparing modified citrus pectin using ultrasonic and microwave assisted pectinase were studied, and the physicochemical properties and structural characteristics of modified and original citrus pectin were characterized by liquid chromatography, Fourier infrared spectroscopy and scanning electron microscopy. The results showed that the degradation efficiency of pectinase could be improved by both ultrasonic and microwave treatment. The weight average molecular weight of the pectin obtained by several degradation methods was 56.18% which was lower than that of the original pectin (283 kDa). Ultrasonic and microwave effect can significantly increase the content of galacturonic acid(GalA), microwave processing enzyme substrates (MEP Ⅰ), microwave digestion product (MEP Ⅱ), ultrasonic treatment enzyme substrates (UEP Ⅰ), ultrasonic treatment enzymolysis products (UEP Ⅱ) GalA content than pectin enzyme solution (39.57%) increased by 52.31%, 46.88%, 66.69% and 61.11% respectively. Moreover, different degradation methods did not change the monosaccharide types of pectin, but made the composition of monosaccharides different. At the same time, it was found that the main chain and side chain of pectin were broken to different degrees after modification, and the degree of esterification was reduced. Scanning electron microscopy (SEM) showed that pectinase, microwave and ultrasound changed the microstructure of pectin in different degrees.
牟方婷,袁美,石黎琳,等. 超声和微波辅助果胶酶处理对果胶结构的影响[J]. 食品与发酵工业, 2021, 47(4): 215-221.
MU Fangting,YUAN Mei,SHI Lilin,et al. Effect of ultrasonic and microwave assisted with pectinase treatment on pectin structure[J]. Food and Fermentation Industries, 2021, 47(4): 215-221.
[1] 程一鑫. 超声波对高甲氧基果胶NMR转折点蔗糖浓度及其结构的影响[D].南昌:南昌大学, 2014. CHENG Y X.Effect of ultrasonic on NMR transition point of sucrose concentration and structure of high methoxy pectin[D].Nanchang:Nanchang University, 2014. [2] 李倩倩,付佳璇,赵玉梅,等.果胶降解与采后果实质地变化研究进展[J].中国食品学报, 2019, 19(9):298-307. LI Q Q, FU J X, ZHAO Y M, et al.Progress on pectin and texture change of postharvest fruits[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(9):298-307. [3] 张丽芬,吴倩,陈复生,等.改性果胶结构、功能及方法的研究进展[J].粮食与油脂, 2015, 28(1):1-5. ZHANG L F, WU Q, CHEN F S, et al.Research on the structure,function and methods of modified pectin[J].Cereals and Oils, 2015, 28(1):1-5. [4] CHEN J, LIANG R H, LIU W, et al.Pectic-oligosaccharides prepared by dynamic high-pressure microfluidization and their in vitro fermentation properties[J].Carbohydr Polym, 2013, 91(1):175-182. [5] 杨蕾. 果胶及低聚糖的制备及其对双歧杆菌体外增殖作用的研究[D].芜湖:安徽工程大学, 2015. YANG L.Reparation of pectins and pectin-oligosaccharides and its effect on Bifidobacterium in vitro[D].Wuhu:Anhui Polytechnic University, 2015. [6] DI R, VAKKALANKA M S, ONUMPAI C, et al.Pectic oligosaccharide structure-function relationships:Prebiotics, inhibitors of Escherichia coli o157:h7 adhesion and reduction of shiga toxin cytotoxicity in ht29 cells[J].Food Chem, 2017, 227:245-254. [7] XU G R, ZHANG C, YANG H X, et al.Modified citrus pectin ameliorates myocardial fibrosis and inflammation via suppressing galectin-3 and TLR4/MYD88/NF-кB signaling pathway[J].Biomed Pharmacother, 2020, 126:110 071. [8] TAKEI TAKAYUKI, SATO MITSUNOBU, IJIMA HIROYUKI, et al.In situ gellable oxidized citrus pectin for localized delivery of anticancer drugs and prevention of homotypic cancer cell aggregation[J].Biomacromolecules, 2010, 11(12):3 525-3 530. [9] YAN J, KATZ AARON.Pectasol-c modified citrus pectin induces apoptosis and inhibition of proliferation in human and mouse androgen-dependent and- independent prostate cancer cells[J].Integr Cancer Ther, 2010, 9(2):197-203. [10] NANGIA-MAKKER PRATIMA, HOGAN VICTOR, HONJO YUICHIRO, et al.Inhibition of human cancer cell growth and metastasis in nude mice by oral intake of modified citrus pectin[J].J Natl Cancer Inst, 2002, 94(24):1 854-1 862. [11] 魏子淏,杨伟,刘夫国,等.改性柑橘果胶研究进展[J].中国食品添加剂, 2014(3):194-200. WEI Z H, YANG W, LIU F G, et al.Advances in modified citrus pectin[J].China Food Additives, 2014(3):194-200. [12] 邱现创,赵宁,李晨,等.铁皮石斛多糖提取工艺优化及对果蝇抗氧化能力的影响[J].食品科学, 2018, 39(2):273-280. QIU X C, ZHAO N, LI C, et al.Optimization of extraction of polysaccharide from Dendrobium officinale and its antioxidant effect on Drosophila melanogaster[J].Food Science, 2018, 39(2):273-280. [13] RAHUL SESHADRI, JOCHEN WEISS, GREG J HULBERT, et al.Ultrasonic processing influences rheological and optical properties of high-methoxyl pectin dispersions[J].Food Hydrocolloids, 2003, 17(2):191-197. [14] 万宇俊,殷军艺,聂少平,等.微波提取对胡萝卜中的多糖基本结构特征及固体形貌的影响[J].食品科学, 2017, 38(7):1-5. WAN Y J, YIN J Y, NIE S P, et al.Impact of microwave extraction on structural characteristics and morphology of carrot (Daucus carota L.) polysaccharide[J].Food Science, 2017, 38(7):1-5. [15] EVZˇENIA DRˇÍMALOVÁ, VLADIMY'R VELEBNY, VLASTA SASINKOVÁ, et al.Degradation of hyaluronan by ultrasonication in comparison to microwave and conventional heating[J].Carbohydrate Polymers, 2005, 61(4):420-426. [16] 廖祥兵,陈晓明,肖伟,等.DNS法定量测定还原糖的波长选择[J].中国农学通报, 2017, 33(15):144-149. LIAO X B, CHEN X M, XIAO W, et al.The wavelength selection of reducing sugar quantitatively determined by DNS method[J].Chinese Agricultural Science Bulletin, 2017, 33(15):144-149. [17] JONGBIN LIM, JIYOUNG YOO, SANGHOON KO, et al.Extraction and characterization of pectin from yuza (citrus junos) pomace:A comparison of conventional-chemical and combined physical-enzymatic extractions[J].Food Hydrocolloids, 2012, 29(1):160-165. [18] YANG X B, ZHAO Y, WANG Q W, et al.Analysis of the monosaccharide components in angelica polysaccharides by high performance liquid chromatography[J].Analytical Sciences, 2005, 21(10):1 177-1 180. [19] 赵文婷. 超高压辅助酶法制备低酯果胶及酶激活机理研究[D].北京:中国农业大学, 2016. ZHAO W T.Preparation and characterization of low methoxyl pectin by high hydrostatic pressure-assisted enzymatic treatment and mechanism of pressure-induced activation[D].Beijing:China Agricultural University, 2016. [20] WANG W J, MA X B, XU Y T, et al.Ultrasound-assisted heating extraction of pectin from grapefruit peel:Optimization and comparison with the conventional method[J].Food Chemistry, 2015, 178:106-114. [21] MANEL MASMOUDI, SOUHAIL BESBES, FATMA ABBES, et al.Pectin extraction from lemon by-product with acidified date juice:Effect of extraction conditions on chemical composition of pectins[J].Food and Bioprocess Technology, 2012, 5(2):687-695. [22] ZHANG L F, YE X Q, XUE SOPHIA JUN, et al.Effect of high-intensity ultrasound on the physicochemical properties and nanostructure of citrus pectin[J].J Sci Food Agric, 2013, 93(8):2 028-2 036. [23] 陈颖. 改性果胶的制备及其结合半乳凝集素-3的结构特征[D].无锡:江南大学, 2016. CHEN Y.Preparation of modified pectin and the structure characteristics of its binding to GAL-3[D].Wuxi:Jiangnan University, 2016. [24] WANG W J, MA X B, JIANG P, et al.Characterization of pectin from grapefruit peel:A comparison of ultrasound-assisted and conventional heating extractions[J].Food Hydrocolloids, 2016, 61:730-739. [25] 马晓彬. 果胶酶促降解中的超声波作用途径及机理研究[D].杭州:浙江大学, 2017. MA X B.Research on function routes and mechanisms of ultrasound in the enzymatic degradation of pectin[D].Hangzhou:Zhejiang University, 2017. [26] AMATJAN SAWUT, MAMATJAN YIMIT, WANFU SUN, et al.Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer[J].Carbohydrate Polymers, 2014, 101:231-239. [27] PAPPAS C S, MALOVIKOVA A, HROMADKOVA Z, et al.Determination of the degree of esterification of pectinates with decyl and benzyl ester groups by diffuse reflectance infrared Fourier transform spectroscopy (drifts) and curve-fitting deconvolution method[J].Carbohydrate Polymers, 2004, 56(4):465-469. [28] KACURÁKOVÁ M, CAPEK P, SASINKOVÁ V, et al.FT-IR study of plant cell wall model compounds:Pectic polysaccharides and hemicelluloses[J].Carbohydr Polym, 2000, 43(2):195-203. [29] SUN J X, SUN R C, SUN X F, et al.Fractional and physico-chemical characterization of hemicelluloses from ultrasonic irradiated sugarcane bagasse[J].Carbohydr Res, 2003, 339(2):291-300.