Comparative genome analysis of carbohydrate activity enzymes zymogram of Lactobacillus from Guangxi pickle
PENG Mingfang1, LI Peijun1,2 *, SHAN Yang1,2,3, CHEN Yuqiu1, YANG Daijun 1, LEI Lichang 1, HUANG Zhihui1, YU Kongxin1
1(College of Chemistry and Bioengineering, Guilin University of technology, Guilin 541004, China) 2(Hunan Academy of Agricultural Sciences, Hunan Agricultural Processing Institute, Changsha 410125, China) 3(Longping Branch Graduate School,Hunan University, Changsha 410125, China)
Abstract: Carbohydrate active enzymes (CAZymes) in microorganisms can degrade plant tissues. Compared with fungi CAZymes, the research on Lactobacillus CAZymes is still relatively scarce. In this study, three lactobacillus strains producing CAZymes were isolated from Guangxi pickle, and their encoding genes were compared by second-generation sequencing. 16S rRNA sequencing showed that these three strains were Lactobacillus brevis DC4, Lactobacillus plantarum GLKK1 and GLKK2. Under liquid-state fermentation, the activities of pectinase, cellulase and hemicellulase of L. brevis DC4 reached (0.40±0.01), (0.04±0.01), and (0.19±0.01) U/mL, respectively. The number of coding sequences (CDS) of DC4, GLKK1 and GLKK2 were 2 615, 3 355 and 3 270, respectively. According to COG annotations, they participated in carbohydrate transport and metabolism, transcription, amino acid transport and metabolism, and so on; CAZymes annotations focused on glycoside hydrolases (GHs), glycosyl transferases (GTs) and carbohydrate esterases (CEs). The three strains contained 17 CAZymes common genes, including cellulase, hemicellulase, pectinase and starch hydrolase and esterase. In addition, L. brevis DC4 contained 10 unique genes, including GH8, GH30 and GH51 families, and L. plantarum GLKK1 and L. plantarum GLKK 2 contained seven unique genes, including GH39, GH13, CE2 and GH78 families.
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2021, Vol. 47 
