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食品与发酵工业  2021, Vol. 47 Issue (1): 79-87    DOI: 10.13995/j.cnki.11-1802/ts.025161
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
耐热克鲁维酵母和戴尔有孢圆酵母在冰葡萄酒酿造中的应用潜力
王婧1,2*, 李敏1,2, 高娉娉1,2, 赵美1,2, 杨婕1,2
1(甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070)
2(甘肃省葡萄与葡萄酒工程学重点实验室,甘肃 兰州,730070)
Application potential of Lachancea thermotolerans and Torulaspora delbrueckii on ice wine fermentation
WANG Jing1,2 *, LI Min1,2, GAO Pingping1,2, ZHAO Mei1,2, YANG Jie1,2
1(College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)
2(Gansu Key Lab of Viticulture and Enology, Lanzhou 730070, China)
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摘要 为了研究耐热克鲁维酵母(Lachancea thermotolerans)和戴尔有孢圆酵母(Torulaspora delbrueckii)在冰葡萄酒酿造中的应用潜力,该文对不同来源的3株L.thermotolerans(LT1、LT2、LT3)和3株T.delbrueckii(TD1、TD2、TD3)菌株的嗜杀性及在冰酒生境下的生长动态、耐受性及其发酵特性进行分析。结果表明:供试的L.thermotoleransT.delbrueckii菌株对冰酒发酵条件均具有良好的适应性;其中菌株LT2和TD2在葡萄糖质量浓度为400~500 g/L、乙醇体积分数为15%、SO2质量浓度为400 mg/L、温度在13~19 ℃的条件下均可保持良好的定殖能力;3株L.thermotolerans和TD2菌株对酿酒酵母1296菌株具有较强的嗜杀性;LT2菌株发酵冰葡萄汁时的乳酸产量最高,为2.195 g/L;LT2和TD2菌株纯种发酵的冰酒乙醇体积分数能达到11%~12%,并且酒样中挥发性酯类、有机酸类物质的总含量比酿酒酵母纯种发酵的酒样显著降低,挥发性萜烯类物质含量显著增加;感官分析表明,TD2菌株纯种发酵的冰葡萄酒有较强花、果香气;LT2菌株能够增强冰葡萄酒的酸度,有助于平衡冰酒的甜腻感,使口感协调。综上所述,LT2和TD2菌株纯种发酵对冰葡萄酒的感官品质有一定的积极影响,L.thermotoleransT.delbrueckii具有酿造冰葡萄酒的应用潜力。
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王婧
李敏
高娉娉
赵美
杨婕
关键词:  耐热克鲁维酵母  戴尔有孢圆酵母  冰葡萄酒  发酵特征  挥发性香气    
Abstract: To study the application potential of Lachancea thermotolerans and Torulaspora delbrueckii in ice wine fermentation, growth parameters including killer property, growth dynamics, tolerance, and fermentation properties of three L. thermotolerans strains (LT1, LT2, LT3) and three T. delbrueckii strains (TD1, TD2, TD3) collected from different sources were evaluated. The results showed that all the tested L. thermotolerans and T. delbrueckii strains had good adaptability for ice wine fermentation conditions, and strains LT2 and TD2 revealed good tolerance to 400-500 g/L glucose, 15% alcohol content, 400 mg/L SO2 at 13-19℃. In addition, strain TD2 and the three L. thermotolerans strains showed strong killer activity to sensitive Saccharomyces cerevisiae strain 1296. In the ice wine fermentation, LT2 strain had the highest lactic acid production (2.195 g/L). The alcohol content of ice wine fermented by LT2 and TD2 strains can reach 11%-12% vol, and the total contents of volatile esters and fatty acids in the ice wine were significantly reduced than the wine samples fermented by Saccharomyces cerevisiae, but the content of volatile terpenes were significantly increased. Sensory analysis showed that the LT2 strain enhanced the acidity to balance the sweetness, helping to harmonize the taste of ice wine, while the TD2 strain strengthened the flower and fruit aroma of the ice wine. It was suggested that either LT2 or TD2 inoculated fermentation have certain positive effects on the sensory quality of ice wine, both L. thermotolerans and T. delbrueckii have the potential to brew ice wine.
Key words:  Lachancea thermotolerans    Torulaspora delbrueckii    ice wine    fermentation properties    volatile aroma
收稿日期:  2020-07-25      修回日期:  2020-08-11                发布日期:  2021-02-03      期的出版日期:  2021-01-15
基金资助: 国家重点研发计划项目(2019YFD1002500);甘肃省高校教师基本科研能力提升项目(2018A-033)
作者简介:  博士,副教授(通讯作者,E-mail: wangjing@gsau.edu.cn)。
引用本文:    
王婧,李敏,高娉娉,等. 耐热克鲁维酵母和戴尔有孢圆酵母在冰葡萄酒酿造中的应用潜力[J]. 食品与发酵工业, 2021, 47(1): 79-87.
WANG Jing,LI Min,GAO Pingping,et al. Application potential of Lachancea thermotolerans and Torulaspora delbrueckii on ice wine fermentation[J]. Food and Fermentation Industries, 2021, 47(1): 79-87.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025161  或          http://sf1970.cnif.cn/CN/Y2021/V47/I1/79
[1] 李景明,马丽艳,杨丽丽.冰葡萄酒及生产工艺[J].中外食品,2005(11):39-41.
LI J M,MA L Y,YANG L L.Ice wine and its production process[J].Global Food Industry,2005(11):39-41.
[2] BENITO Á,CALDERON F,PALOMERO F,et al.Quality and composition of Airén wines fermented by sequential inoculation of Lachancea thermotolerans and Saccharomyces cerevisiae[J].Food Technology and Biotechnology,2015,54(2):135-144.
[3] PETRUZZI L,CAPOZZI V,BERBEGAL C,et al.Microbial resources and enological significance:Opportunities and benefits[J].Frontier in Microbiology,2017,8:995.
[4] 谭凤玲,王宝石,胡培霞,等.非酿酒酵母在葡萄酒混菌发酵中的应用及其挑战[J/OL].食品与发酵工业,2020.DOI:10.13995/j.cnki.11-1802/ts.024520.
TAN F L,WANG B S,HU P X,et al.Application and challenge of non-Saccharomyces cerevisiae yeast in the mixed fermentation of wine[J/OL].Food and Fermentation Industries,2020.DOI:10.13995/j.cnki.11-1802/ts.024520.
[5] KURTZMAN C P,ROBNETT C J.Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses[J].FEMS Yeast Res,2003,3:417-432.
[6] BENITO S.The impacts of Lachancea thermotolerans yeast on winemaking[J].Applied Microbiology and Biotechnology,2018,102:6 775-6 790.
[7] TRISTAN J P,BENOIT D,MATHABATHA E S.Lachancea yeast species:Origin,biochemical characteristics and oenological significance[J].Food Research International,2019,119:378-389.
[8] 王玉华,盛文军,李敏,等.耐热克鲁维酵母和粟酒裂殖酵母顺序接种发酵对美乐干红葡萄酒品质的影响[J].食品科学,2019,40(8):102-111.
WANG Y H,SHENG W J,LI M,et al.Effect of sequential fermentation with Lachancea thermotolerans and Schizosaccharomyces pombe on the quality of Merlot dry red wine[J].Food Science,2019,40(8):102-111.
[9] SHEKHAWAT K,PORTER T J,Bauer F F,et al.Employing oxygen pulses to modulate Lachancea thermotolerans-Saccharomyces cerevisiae Chardonnay fermentations[J].Annals of Microbiology,2018,68(2):93-102.
[10] PONSONE M L,NALLY,et al.Evaluation of the effectiveness of potential biocontrol yeasts against black sur rot and ochratoxin A occurring under greenhouse and field grape production conditions[J].Biological Control,2016,103:78-85.
[11] BENITO Á,CALDERON F,BENITO S.Combined use of S.pombe and L.thermotolerans in winemaking.beneficial effects determined through the study of wines’ analytical characteristics[J].Molecules,2016,21(12):1 744
[12] BENITO Á,CALDERON F,PALOMERO F,et al.Combine use of selected Schizosaccharomyces pombe and Lachancea thermotolerans yeast strains as an alternative to the traditional nalolactic fermentation in red wine production[J].Molecules (Basel,Switzerland),2015,20(6):9 510-9 523.
[13] COMITINI F,GOBBI M,DOMIZIO P,et al.Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae[J].Food Microbiology,2011,28(5):873-882.
[14] BELY M,STOECKLE P,MASNEUF P L,et al.Impact of mixed Torulaspora delbrueckii-Saccharomyces cerevisiae culture on high-sugar fermentation[J].International Journal of Food Microbiology,2008,122(1):312-320.
[15] BELDA I,RUIZ J,BEISERT B,et al.Influence of Torulaspora delbrueckii in varietal thiol (3-SH and 4-MSP) release in wine sequential fermentations[J].International Journal of Food Microbiol,2017,257:183-191.
[16] BENITO S.The impacts of Torulaspora delbrueckii yeast on winemaking[J].Applied Microbiology and Biotechnology,2018,102(7):3 081-3 094.
[17] ULLIVARRI M,MENDOZA L M,RAYA R R.Killer activity of Saccharomyces cerevisiae strains:Partial characterization and strategies to improve the biocontrol efficacy in winemaking[J].Antonie Van Leeuwenhoek,2014,106(5):865-878.
[18] 杨婕,王玉华,米兰,等.耐热克鲁维酵母与酿酒酵母顺序接种发酵对霞多丽干白葡萄酒感官品质的影响[J].食品与发酵工业,2019,45(18):144-154.
YANG J,WANG Y H,MI L,et al.Effects of sequential fermentation of Lachancea thermotolerans and Saccharomyces cerevisiae on the quality of Chardonnay dry white wine[J].Food and Fermentation Industries,2019,45(18):144-154.
[19] 刘灿珍,董书甲,姜凯凯,等.5株非酿酒酵母的耐受性及发酵特性研究[J].中国酿造,2017,36(10):42-46.
LIU C Z,DONG S J,JIANG K K, et al.Tolerance and fermentation characteristics of five non-Saccharomyces[J].China Brewing,2017,36(10):42-46.
[20] BUGLASS A J,LEE S H.SEQUENTIAL.Analysis of malic acid and both enantiomers of lactic acid in wine using a high-performance liquid chromatographic column-switching procedure[J].Journal of Chromatographic Science,2001,39(11):453-458.
[21] PEREZ-RUIZ T,MART N C,TOMAS V,et al.High-performance liquid chromatographic separation and quantification of citric,lactic,malic,oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection[J].Journal of Chromatography A,2004,1026(1-2):57-64.
[22] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 15038—2006 葡萄酒、果酒通用分析方法[S].北京:中国标准出版社,2006.
State Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,China National Standardization Administration.GB/T 15038—2006 Analytical methods of wine and fruit wine[S].Beijing:China Standards Press,2006.
[23] 曲昆生,曲天波,曲林,等.苹果酸-乳酸发酵对威代尔冰酒香气的影响[J].中国酿造,2019,38(9):36-42.
QU K S,QU T B,QU L,et al.Effect of malolactic fermentation on volatile compounds of Vidal ice wine[J].China Brewing,2019,38(9):36-42.
[24] PEREZ-MAGARINO S,ORTEGA-HERAS M,CANO-MOZO E,et al.The influence of oak wood chips,micro-oxygenation treatment,and grape variety on colour,and anthocyanin and phenolic composition of red wines[J].Journal of Food Composition & Analysis,2009,22(3):204-211.
[25] 苑伟,王学锋,刘延琳.优选酿酒酵母菌株发酵性能研究[J].中国酿造,2010,29(9):48-52.
YUAN W,WANG X F,LIU Y L,Study of the vinification characteristics of superior wine yeast[J].China Brewing,2010,29(9):48-52.
[26] 杨诗妮,叶冬青,贾红帅,等.本土戴尔有孢圆酵母在葡萄酒酿造中的应用潜力[J].食品科学,2019,40(18):108-115.
YANG S N,YE D Q,JIA H S,et al.Oenological potential of indigenous Torulaspora delbrueckii for winemaking[J].Food Science,2019,40(18):108-115.
[27] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T25 504—2010 冰葡萄酒[S].北京:中国标准出版社,2010.
State Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,China National Standardization Administration.GB/T25 504—2010 Ice Wines[S].Beijing:China Standards Press,2010.
[28] 李华,王华,袁春龙,等.葡萄酒化学[M].北京:科学出版社,2006.
LI H,WANG H,YUAN C L,et al.Wine Chemistry[M].Beijing:Science Press,2006.
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