Abstract: Hexose oxidase (HOX) has a wide substrate spectrum, which makes it quite different from glucose oxidase. HOX catalyzes the oxidation of mono- and disaccharides to their corresponding lactones, including D-glucose, D-fructose, D-galactose, maltose, and lactose, with concomitant reduction of molecular oxygen to hydrogen peroxide. In recent years, HOX had been shown to have great application potential as a novel green additive in food, feeding and other industries due to its function of deoxygenation, acidification and improvement of protein cross-linking. HOX is primarily derived from red algae species. Current production of HOX is mainly dependent upon the isolation from the natural source. Studies have shown active expression and fermentation production in genetic engineered yeast. This review focused on the separation, purification, enzymatic properties, and heterologous expression of HOX, as well as some application cases, as to provide references for its development and application.
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