Please wait a minute...
 
 
食品与发酵工业  2021, Vol. 47 Issue (7): 109-115    DOI: 10.13995/j.cnki.11-1802/ts.025314
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
厚壳贻贝多糖对葡聚糖硫酸钠诱导的结肠炎改善作用
姚丽文1,2, 周宇芳2, 孙继鹏2, 王家星2, 廖妙飞2, 郑斌1,2, 王芮3, 邓尚贵1*, 相兴伟2,3*
1(浙江海洋大学 食品与医药学院,浙江 舟山,316021)
2(浙江省海洋开发研究院,浙江 舟山,316021)
3(浙江工业大学 食品科学与工程学院,浙江 杭州,310014)
Improving effect of mussel (Mytilus corusus) polysaccharide on dextran sodium sulfate-induced colitis
YAO Liwen1,2, ZHOU Yufang2, SUN Jipeng2, Wang Jiaxing2, LIAO Miaofei2, ZHENG Bin1,2, WANG Rui3, DENG Shanggui1*, XIANG Xingwei2,3*
1(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316021,China)
2(Zhejiang Marine Development Institute,Zhoushan 316021,China)
3(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China)
下载:  HTML   PDF (3256KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 为了探讨厚壳贻贝多糖(Mytilus coruscus polysaccharide,MP)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导的结肠炎的改善作用,试验设置空白组、模型组和MP组(300 mg/kg),在日常饮用水中给予质量分数3.5% DSS构建结肠炎模型,通过观察小鼠体态表征、肠道组织形态、测定肠道和血清细胞因子水平,研究MP对小鼠结肠炎的影响。结果表明:与模型组相比,MP组显著缓解了小鼠体重的降低、结肠长度的缩短(P<0.05),并降低疾病活动指数,改善炎性细胞浸润、腺体水肿以及杯状细胞的减少,MP组结肠组织中超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽还原酶(flutathione peroxidase,GSH-Px)以及血清中的GSH-Px活性显著增高(P<0.05),血清中的SOD有所升高但无显著性差异。结肠组织和血清中的肿瘤坏死因子-α含量显著降低(P<0.05),白细胞介素-6含量有所降低但无显著性差异。该研究证明MP能在一定程度上改善由DSS诱导的结肠炎。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
姚丽文
周宇芳
孙继鹏
王家星
廖妙飞
郑斌
王芮
邓尚贵
相兴伟
关键词:  厚壳贻贝多糖  结肠炎  葡聚糖硫酸钠  抗氧化  炎症反应    
Abstract: In this experiment, three groups:blank control group, model group and Mytilus coruscus polysaccharide (MP) group (300 mg/kg) were set up to explore whether MP could improve colitis induced by dextran sodium sulfate (DSS).After 3.5% DSS was given in daily drinking water, the drug was administrated by gavage, and the improvement effects of MP on colitis in mice were studied by observing the body characterization of mice, intestinal tissue morphology, and determination of intestinal and serum cytokines.The results showed that compared with the model group, the MP group could significantly alleviate the weight loss of mice, the shortening of the colon length (P<0.05), and reduce the disease activity index, improve inflammatory cell infiltration, glandular edema and goblet cell cut back.Compared with the model group, the superoxide dismutase (SOD) and glutathione reductase (GSH-Px) in the colon tissue of the MP group and the GSH-Px activity in the serum were significantly increased (P<0.05), and the SOD in the serum increased but there was no significant difference.The content of inflammatory factor tumor necrosis factor-ɑ(TNF-ɑ)in colon tissue and serum was significantly reduced (P<0.05), and the content of interleukin-6(IL-6)was reduced but there was no significant difference.The results proved that the Mytilus coruscus polysaccharide could improve the colitis induced by DSS to a certain extent.
Key words:  Mytilus coruscus polysaccharide    colitis    dextran sodium sulfate    antioxidant    inflammation
收稿日期:  2020-08-09      修回日期:  2020-09-08           出版日期:  2021-04-15      发布日期:  2021-05-20      期的出版日期:  2021-04-15
基金资助: 浙江省重点研发计划项目(2019C02076);舟山市“五大会战”科技项目(2017C12030);浙江省公益性项目(LGN18C200006);浙江省教育厅一般科研项目(Y202043175)
作者简介:  硕士研究生(邓尚贵教授和相兴伟教授为共同通讯作者,E-mail:dengshanggui@163.com;xxw11086@zjut.edu.cn)
引用本文:    
姚丽文,周宇芳,孙继鹏,等. 厚壳贻贝多糖对葡聚糖硫酸钠诱导的结肠炎改善作用[J]. 食品与发酵工业, 2021, 47(7): 109-115.
YAO Liwen,ZHOU Yufang,SUN Jipeng,et al. Improving effect of mussel (Mytilus corusus) polysaccharide on dextran sodium sulfate-induced colitis[J]. Food and Fermentation Industries, 2021, 47(7): 109-115.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025314  或          http://sf1970.cnif.cn/CN/Y2021/V47/I7/109
[1] 杨小冰,金明玉,吴小禾,等.膳食营养素与炎症性肠病关系研究进展[J].食品科学,2019,40(9):317-323.YANG X B,JIN M Y,WU X H,et al.Research progress on the relationship between dietary nutrients and inflammatory bowel disease[J].Food Science,2019,40(9):317-323.
[2] 郭小芹. IL-17/IL-17R通路在炎症性肠病中的免疫调节机制研究[D].北京:中国人民解放军军事医学科学院,2013.GUO X Q.The immunoregulatory mechanism of IL-17/IL-17R pathway in inflammatory bowel disease[D].Beijing:Chinese Academy of Military Medical Sciences,2013.
[3] 李亮成. 炎症性肠病的临床表现[J].山西医药杂志,1993(2):93-94.LI L C.Clinical manifestations of inflammatory bowel disease[J].Shanxi Medical Journal,1993(2):93-94.
[4] 邓长生, 陈志芬.炎症性肠病的临床表现、诊断及鉴别诊断[J].实用医学杂志,2003(5):9-10.DENG C S,CHEN Z F.Clinical manifestations,diagnosis and differential diagnosis of inflammatory bowel disease[J].The Journal of Practical Medicine,2003(5):9-10.
[5] ZSUZSANNA V,ZSUZSANNA K,PETER L L.Epidemiology of inflammatory bowel diseases from west to east[J].Journal of Digestive Diseases,2017,18(2):92-98.
[6] 兰艳. 脂多糖对葡聚糖硫酸钠所致炎症性肠病的影响及机制研究[D].武汉:华中科技大学,2012.LAN Y.Effect of lipopolysaccharide on inflammatory bowel disease caused by dextran sodium sulfate and its mechanism[D].Wuhan:Huazhong University of Science and Technology,2012.
[7] 魏江洲, 张建鹏,刘军华,等.海螵蛸多糖CPS-1对小鼠实验性溃疡性结肠炎作用的初步观察[J].第二军医大学学报,2006,27(1):28-30.WEI J Z,ZHANG J P,LIU J H,et al.Preliminary observation on the effect of cuttlefish polysaccharide CPS-1 on experimental ulcerative colitis in mice[J].Journal of Second Military Medical University,2006,27(1):28-30.
[8] 王百亿. 海带所含昆布多糖能防肠炎[J].世界最新医学信息文摘,2018,18(32):10.WANG B Y.Laminaria polysaccharide contained in kelp can prevent enteritis[J].Digest of World Latest Medical Information,2018,18(32):10.
[9] 周文丽. 东海厚壳贻贝多糖的抗衰老生物学活性研究[D].上海:第二军医大学,2009.Zhou W L.Study on the anti-aging biological activity of polysaccharides from the East China Sea mussels[D].Shanghai:Second Military Medical University,2009.
[10] 侯重文, 朱梓昂,张金华,等.高效凝胶色谱法测定贻贝多糖含量及分子量[J].中国生化药物杂志,2016,36(11):9-12.HOU C W,ZHU Z A,ZHANG J H,et al.Determination of mussel polysaccharide content and molecular weight by high performance gel chromatography[J].Chinese Journal of Biochemical Pharmaceutics,2016,36(11):9-12.
[11] 徐红丽. 东海厚壳贻贝水溶性多糖MP-Ⅰ的结构鉴定及生物学活性研究[D].上海:第二军医大学,2007.XU H L.Study on the structure identification and biological activity of the water-soluble polysaccharide MP-Ⅰ from the east China sea mussel mussels[D].Shanghai:Second Military Medical University,2007.
[12] 李江滨, 侯敢.翡翠贻贝多糖免疫调节作用研究[J].食品研究与开发,2009(5):10-12.LI J B,HOU G.Study on the immunomodulatory effect of polysaccharides from emerald mussel[J].Food Research and Development,2009(5):10-12.
[13] YANG R H.Investigation on extraction of mussel juice polysaccharides and their biological activities[J].Journal of Chinese Institute of Food Science & Technology,2009 (1):13-14.
[14] 周越. 贻贝肽与贻贝多糖对衰老的干预作用及其机制[D].镇江:江苏大学,2013.ZHOU Y.Interventional effects of mussel peptides and mussel polysaccharides on aging and its mechanism[D].Zhenjiang:Jiangsu University,2013.
[15] ZHANG H Y,LUO G H,HAO J Y,et al.Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from Cynomorium songaricum and their antitumor activity[J].Food Science,2016:895-902.
[16] WU J X,SHAO H R,ZHANG J H,et al.Mussel polysaccharide α-D-glucan (MP-A) protects against non-alcoholic fatty liver disease via maintaining the homeostasis of gut microbiota and regulating related gut-liver axis signaling pathways[J].International Journal of Biological Macromolecules,2019,130:68-78.
[17] 李孟婕, 范秀萍,吴红棉,等.翡翠贻贝粗多糖降血脂作用的研究[J].食品科学,2012,33(1):264-268.LI M J,FAN X P,WU H M,et al.Study on the hypolipidemic effect of the crude polysaccharide of Emerald mussel[J].Food Science,2012,33(1):264-268.
[18] 姚滢.东海厚壳贻贝多糖的分离提纯和免疫学活性研究[D].上海:第二军医大学,2005.YAO Y.Isolation,purification and immunological activity of polysaccharides from the east China sea mussels[D].Shanghai:Second Military Medical University,2005.
[19] 栾洁, 辛甜,储智勇,等.贻贝多糖对小鼠免疫功能的调节作用[J].中国海洋药物,2010(4):43-45.LUAN J,XIN T,CHU Z Y,et al.The regulatory effect of mussel polysaccharide on the immune function of mice[J].China Marine Medicine,2010(4):43-45.
[20] ZURONG T,KAIDA X,SHUOBO L,et al.Effects of low concentrations copper on antioxidant responses,DNA damage and genotoxicity in thick shell mussel Mytilus coruscus[J].Fish & Shellfish Immunology,2018,82:77-83.
[21] 付元庆. 厚壳贻贝脂质提取物对肠道损伤、溃疡性结肠炎以及类风湿性关节炎的影响和其机理研究[D].杭州:浙江大学,2015.FU Y Q.Study on the effect and mechanism of the lipid extract of mussel thick shell on intestinal injury,ulcerative colitis and rheumatoid arthritis[J].Hangzhou:Zhejiang University,2015.
[22] WENWEI CHEN,ZHENBAO JIA,JIAJIE ZHU,et al.Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from thick-shell mussel (Mytilus coruscus) and their antioxidant activities[J].International Journal of Biological Macromolecules,2019,140:1 116-1 125.
[23] 刘淑集, 罗钦钦,潘裕添,等.翡翠贻贝不同醇沉物体外抗氧化活性研究[J].食品与发酵工业,2017,43(6):187-192.LIU S J,LUO Q Q,PAN Y T,et al.Study on antioxidant activity in different alcohol precipitation of emerald mussel[J].Food and Fermentation Industries,2017,43(6):187-192.
[24] ROBIN G L,VANCE J M,CHARLES O E.Animal models of intestinal inflammation:Ineffective communication between coalition members[J].Springer Seminars in Immunopathology,2005,27(2):233-247.
[25] PODOLSKY D K,XAVIER R J.Unravelling the pathogenesis of infIammatory bowel disease[J].Nature Protocols,2007,448(7 152):427-434.
[26] YAN H,ZHAO J M,XIN G,et al.Effect of warming moxibustion on P2RX_7 regulation in tianshu acupoint skin of rats with ulcerative colitis[J].Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2016(6),245.
[27] STEFAN W,VANESSA P,MARKUS K,et al.Chemically induced mouse models of acute and chronic intestinal inflammation[J].Nature Protocols,2017,12(7):1 295-1 309.
[28] 徐丽红, 肖芳,兰小琴,等.葡聚糖硫酸钠诱导C57BL/6 J小鼠急性结肠炎模型的建立与评价[J].医学研究生学报,2014,27(9):918-922.XU L H,XIAO F,LAN X Q,et al.Establishment and evaluation of acute colitis model in C57BL/6 J mice induced by dextran sodium sulfate[J].Journal of Medical Postgraduates,2014,27(9):918-922.
[29] TOIYAMA Y,MIKI C,INOUE Y,et al.Evaluation of an inflammation-based prognostic score for the identification of patients requiring postoperative adjuvant chemotherapy for stage II colorectal cancer[J].Experimental & Therapeutic Medicine,2011,2(1):95-101.
[30] 曾江宁, 彭玲,黄伟,等.厚壳贻贝抗氧化酶活性和脂质过氧化物含量对Cd和Aroclor1254胁迫的响应[J].生态学杂志,2015,34(3):718-726.ZENG J N,PENG L,HUANG W,et al.Antioxidant enzyme activity and lipid peroxidation content of mussel thick shell in response to Cd and aroclor 1254 stress[J].Chinese Journal of Ecology,2015,34(3):718-726.
[31] LI Q,XIONG C,CHEN C,et al.Neuroprotective effect of crude polysaccharide isolated from the fruiting bodies of Morchella importuna against H2O2-induced PC12 cell cytotoxicity by reducing oxidative stress[J].Biomedicine & Pharmacotherapy,2016,83:569-576.
[32] 浦江, 王少鑫,刘超群,等.炎症因子TNF-α、IL-6和IL-4在溃疡性结肠炎中的表达及临床意义[J].胃肠病学和肝病学杂志,2015,3:104-106.PU J,WANG S X,LIU C Q,et al.Expression and clinical significance of inflammatory factors TNF-α,IL-6 and IL-4 in ulcerative colitis[J].Stomach Journal of Enterology and Hepatology,2015,3:104-106.
[33] WU X X,GUO W J,WU L M,et al.Selective sequestration of STAT1 in the cytop lasm via phosphorylated SHP-2 amel iorates murine exper imental colitis[J].Journal of Immunology,2019,189(7):3 497-3 507.
[34] KRETZMANN N A,FILLMANNH H,JOSÉ L M,et al.Effects of glutamine on proinflannatory gene expression and activation of nuclear factor kappa B and signal transducers and activators of transcription in TNBS-induced colitis[J].Inflammatory Bowel Diseases,2010,14(11):1 504-1 513.
[35] LIU Y,DU Y Q,WANG J H,et al.Structural analysis and antioxidant activities of polysaccharide isolated from Jinqian mushroom[J].International Journal of Biological Macromolecules,2014(64):63-68.
[36] 蔡洁珠,陈宁.炎症性肠病治疗的实验室监测[J].中华检验医学杂志,2018,41(10):781-783.CAI J Z,CHEN N.Laboratory monitoring for the treatment of inflammatory bowel disease[J].Chinese Journal of Laboratory Medicine,2018,41(10):781-783.
[1] 张涛, 邓思, 陈艳红, 杜希萍. 虾青素和β-胡萝卜素的抗氧化活性及其协同作用研究[J]. 食品与发酵工业, 2021, 47(9): 8-15.
[2] 冯艳钰, 臧延青. 三种小麦麸皮总黄酮的体外抗氧化活性[J]. 食品与发酵工业, 2021, 47(9): 16-24.
[3] 刘晓晨, 杨光, 杨波, 周盛敏. 光照萌发对亚麻籽油中脂质伴随物含量的影响[J]. 食品与发酵工业, 2021, 47(9): 208-214.
[4] 余祥英, 陈晓纯, 李玉婷, 李琳. 陈皮挥发油组成分析及其单体的抗氧化性研究[J]. 食品与发酵工业, 2021, 47(9): 245-252.
[5] 曹联飞, 何程豪, 孙玉敬. 大蜜蜂和黑大蜜蜂蜂蜜的理化指标及抗氧化活性分析[J]. 食品与发酵工业, 2021, 47(9): 262-267.
[6] 王春幸, 张东, 贺稚非, 李芳, 陈茹, 李洪军. 天然保鲜剂的作用机理及其在调理肉制品中的应用研究进展[J]. 食品与发酵工业, 2021, 47(9): 328-334.
[7] 牛娜娜, 沙如意, 杨陈铭, 王珍珍, 茹语婷, 戴静, 韩洪庚, 张黎明, 毛建卫. 预处理工艺对黑蒜功能性成分、抗氧化活性影响及相关性研究[J]. 食品与发酵工业, 2021, 47(8): 67-75.
[8] 郭佳, 李云飞, 李诗佳, 关红霞, 陈晓光. 酸浆宿萼抗衰老作用及机理研究[J]. 食品与发酵工业, 2021, 47(8): 140-144.
[9] 孟洋, 卢红梅, 杨双全, 章之柱, 陈莉, 刘兵, 王利萍. 铁皮石斛复配花茶制作工艺及其功能性研究[J]. 食品与发酵工业, 2021, 47(8): 170-179.
[10] 王子涵, 向敏, 徐茂, 蒋和体. 响应面优化黑果腺肋花楸汁澄清工艺及其抗氧化活性评价[J]. 食品与发酵工业, 2021, 47(8): 189-196.
[11] 顾欣, 高涛, 刘梦雅, 丛之慧, 张诚雅, 肖乐艳, 李迪, 胡景涛. 梁平柚柚皮多糖的提取、结构解析及抗氧化能力研究[J]. 食品与发酵工业, 2021, 47(7): 137-145.
[12] 陈思雨, 梁鑫, 雷钰, 孔倩倩, 万欣, 张宝善. 豆腐黄浆水发酵阶段产物抗氧化成分和色泽变化[J]. 食品与发酵工业, 2021, 47(6): 34-41.
[13] 赵昊, 宋晶晶, 于佳俊, 张晓蒙, 张凤杰, 李涛, 武运, 薛洁. 不同产区葡萄酒多酚物质抗氧化活性差异及相关性分析[J]. 食品与发酵工业, 2021, 47(6): 84-91.
[14] 李江阔, 高静, 张鹏, 霍俊伟. 微环境气调对蓝果忍冬贮藏品质和抗氧化酶的影响[J]. 食品与发酵工业, 2021, 47(6): 152-159.
[15] 张耀, 张露, 刘俊, 涂宗财. 青鱼肉活性肽的制备及其抗肿瘤活性研究[J]. 食品与发酵工业, 2021, 47(5): 35-42.
No Suggested Reading articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
版权所有 © 《食品与发酵工业》编辑部
地址:北京朝阳区酒仙桥中路24号院6号楼111室
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn