Abstract: Tyrosinase is a key enzyme in browning and melanin biosynthesis of fruit and vegetable, and tyrosinase inhibitors are of great significance in the field of fruit and vegetable preservation, skin whitening and medicine. In this study, ribavirin was first evaluated for its anti-tyrosinase activity and application in the preservation of Gong pear. The results of enzyme kinetics experiments indicated that ribavirin was an efficient [IC50 (0.3±0.05) mmol/L], reversible, and competitive type inhibitor of tyrosinase. Nonradiative energy transfer analysis and fluorescence quenching assays demonstrated that ribavirin formed ribavirin-enzyme complex with tyrosinase through one binding site, and accompanied by the change of enzyme conformation and nonradiative energy transfer. The results of molecular docking showed that ribavirin could enter the active center of tyrosinase and form hydrogen bonds with its six amino acid residues (Lys379, Gln356, Gln307, Asp312, Val313, Asn310) on the B chain. The experiment of Gong pears preservation showed that ribavirin could effectively reduce the weight-loss rate of fresh-cut pears and the browning degree of pear juice. The results provided theoretical and practical basis for the development of novel fruit and vegetable preservatives.
邓维良,柴纬明,罗麟霜. 利巴韦林抗酪氨酸酶活性及其在贡梨中的保鲜应用[J]. 食品与发酵工业, 2021, 47(5): 162-167.
DENG Weiliang,CHAI Weiming,LUO Linshuang. Anti-tyrosinase activity of ribavirin and its application in Gong pear preservation[J]. Food and Fermentation Industries, 2021, 47(5): 162-167.
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