New technology of total nutrient feeding fermentation of glutamic acid
LIU Jingyang1, LIU Yunpeng1, XU Qingyang1,2,3*
1(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China) 2(Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture,Tianjin 300457,China) 3(National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin 300457,China)
Abstract: Total nutrient feeding mainly refers to feeding a suitable total nutrient medium at an appropriate time to supplement the nutrients consumed by bacterial growth and metabolism. Adopting the strategy of total nutrient feeding can also reduce the concentration of the fermentation medium to avoid the inhibition of eutrophication to the cell viability. Therefore, adopting a total nutrient feeding strategy can solve the problems of insufficient cell viability and decreased acid production capacity in the late stage of L-glutamic acid fermentation. Through experimental analysis, it was determined that the optimal feeding condition was to start feeding with a volume fraction of 60% for 24 h after 2 h of fermentation. Under this condition, the biomass reached 66, which was increased by 29.4%, the transformation time of the bacteria was advanced by 2 h, and the L-glutamic acid production reached 168 g/L, which was an increase of 22.6%. The lactic acid content was 3.1 g/L, decreased by 13.8%; the alanine content was 2.06 g/L, with a reduction of 17.6%, and the sugar-acid conversion rate was 63%, with an increase of 1.5%. Total nutrient feeding fermentation has a positive effect on accelerating the transformation of the bacteria, improving the cell viability, increasing the production of L-glutamic acid and sugar acid conversion rate.
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2021, Vol. 47 
