Microbial diversity of wine grape skins from four different regions in China
ZHAO Yu1, LI Jingyuan2, XIE Wancui1, ZHAO Hongwei1*
1(College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao 266045,China) 2(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)
Abstract: The natural microorganisms on the skin of wine grapes could directly affect the aroma and taste of wine. Cabernet Sauvignon grapes from four representative regions in central and eastern China were selected to study the microbial diversity of grape skins using high-throughput sequencing technology. Bioinformatics analysis of the sequencing data showed that the dominant bacteria were Proteobacteria and the dominant fungi were Ascomycota and Basidiomycota at the phylum level in the four regions. At the genus level, Phenylobacterium was the dominant bacteria in the samples from the regions of Huailai in Hebei, Qingdao in Shandong and Helan Mountain in Ningxia, while it was Ralstonia in Penglai. The dominant fungi in Helan Mountain, Qingdao and Penglai samples were Aureobasidium. Moreover, Rhodotorula also showed higher abundance in Qingdao and Penglai samples. The results confirmed that the dominant microorganisms on the grape skin showed obvious differences due to different geographic locations. In addition, the difference on genus level was higher than that on phylum level. This may be related to natural and man-made factors such as climate, water and cultivation conditions.
赵昱,李静媛,解万翠,等. 四个不同产区酿酒葡萄果皮微生物多样性的研究[J]. 食品与发酵工业, 2021, 47(11): 53-60.
ZHAO Yu,LI Jingyuan,XIE Wancui,et al. Microbial diversity of wine grape skins from four different regions in China[J]. Food and Fermentation Industries, 2021, 47(11): 53-60.
CAPECE A,ROMANIELLO R,SIESTO G,et al.Selection of indigenous Saccharomyces cerevisiae strains for Nero d'Avola wine and evaluation of selected starter implantation in pilot fermentation[J].International Journal of Food Microbiology,2010,144(1):187-192.
魏玉洁, 邹弯,马文瑞,等.应用高通量测序技术研究新疆产区葡萄果实、叶片及果园土壤微生物多样性[J].食品科学,2018,39(6):162-170.WEI Y J,ZOU W,MA W R,et al.Microbial diversity of berries,leaves and soil of grapevine plants grown in Xinjiang analyzed by high-throughput sequencing[J].Food Science,2018,39(6):162-170.
许维娜, 刘希萌,孔祥君,等.田间施药对自然发酵葡萄酒酵母菌群落结构的影响[J].微生物学通报,2019,46(4):721-728.XU W N,LIU X M,KONG X J,et al.Effect of pesticide application on the community structure of wine yeasts during spontaneous fermentation[J].Microbiology Sinica,2019,46(4):721-728.
张世伟, 陈曦,钟其顶,等.不同品种酿酒葡萄表皮微生物群落多样性分析[J].生物技术通报,2017,33(3):128-137.ZHANG S W,CHEN X,ZHONG Q D,et al.Microbial communities on the wine grape surfaces of different cultivars[J].Biotechnology Bulletin,2017,33(3):128-137.
王晓雯. 贵州地区葡萄园土壤微生物多样性研究[D].广州:华南理工大学,2017.WANG X W.Study on microbial diversity in vineyard soil from regions of Guizhou[D].Guangzhou:South China University of Technology,2017.
张俊杰, 郭晨,尚益民,等.市售夏黑无核葡萄果表微生物多样性研究[J].轻工学报,2017,32(6):20-26.ZHANG J J,GUO C,SHANG Y M,et al.Study on the microbial diversity on the grape surface of marketed summer black seedless variety[J].Journal of Light Industry,2017,32(6):20-26.
WANG X H,XU X,BAO Z,et al.High-throughput sequencing analysis of microbial diversity in the combined fermentation stages of cow dung and corn straw[J].Food and Fermentation Industries,2019,45(3):47-55.
BOKULICH N A,THORNGATE J H,RICHARDSON P M,et al.Microbial biogeography of wine grapes is conditioned by cultivar,vintage,and climate[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(1):E139-E148.
MORGAN H H,DU T M,SETATI M E.The grapevine and wine microbiome:Insights from high-throughput amplicon sequencing[J].Frontiers in Microbiology,2017,8:820.
INAM U K,HABIB N,XIAO M,et al.Phenylobacterium terrae sp.nov.,isolated from a soil sample of Khyber-Pakhtun-Khwa,Pakistan[J].Antonie van Leeuwenhoek,2018,111(10):1 767-1 775.
CHANDRASEKARAN M,SUBRAMANIAND,YOON E,et al.Meta-analysis reveals that the genus Pseudomonas can be a better choice of biological control agent against bacterial wilt disease caused by Ralstonia solanacearum [J].The Plant Pathology Journal,2016,32(3):216-227.
SIROVA D,SANTRUCEK J,ADAMEC L,et al.Dinitrogen fixation associated with shoots of aquatic carnivorous plants:Is it ecologically important?[J].Annals of Botany,2014,114(1):125-133.
YANG J R,WANG Y,CHEN H,et al.Ammonium removal characteristics of an acid-resistant bacterium Acinetobacter sp.JR1 from pharmaceutical wastewater capable of heterotrophic nitrification-aerobic denitrification[J].Bioresource Technology,2019,274:56-64.
张世伟.酿酒葡萄微生物群落多样性及其氮代谢通量研究[D].北京:中国矿业大学(北京),2018.ZHANG S W.Study on microbial community diversity and the nitrogen metabolism flux of wine grapes[D].Beijing:China University of Mining and Technology(Beijing),2018.
杨敏, 殷绒,张国涛,等.基于高通量测序技术的香格里拉葡萄酒产区根际微生物多样性研究[J].云南农业大学学报(自然科学版),2020,35(3):392-400.YANG M,YIN R,ZHANG G T,et al.Study on the grape rhizosphere microbial diversity in Shangri-La wine region by high-throughput sequencing technology[J].Journal of Yunnan Agricultural University(Natural Science Edition),2020,35(3):392-400.
刘思. 贺兰山东麓葡萄园行内覆盖对微域生态环境、葡萄及葡萄酒品质的影响[D].银川:宁夏大学,2019.LIU S,Effects of within-row mulching on micro-ecological environment grape and wine quality in vineyards of eastern foothill of Helan mountain[D].Yinchuan:Ningxia University,2019.
焦红茹. 不同酿酒葡萄品种相关酵母菌的分离及分类鉴定[D].杨凌:西北农林科技大学,2008.JIAO H R.Isolation and identification of wine-related yeast associated to different varieties[D].Yangling:Northwest A&F University,2008.
侯芳彬, 王蕊,SALMAN A,等.黄土区沟道泥沙微生物群落变化特征及其影响因素[J].中国环境科学,2019,39(10):4 350-4 359.HOU F B,WANG R,SALMAN A,et al.Variations of soil microbial communities along a valley bottom of the loess plateau and the influencing factors[J].China Environmental Science,2019,39(10):4 350-4 359.
LEVEAU J H J,TECH J J.Grapevine microbiomics:Bacterial diversity on grape leaves and berries revealed by high-throughput sequence analysis of 16S rRNA amplicons[J].Acta Horticulturae,2011(905):31-42.
MARTINS G,LAUGA B,MIOT-SERTIER C,et al.Characterization of epiphytic bacterial communities from grapes,leaves,bark and soil of grapevine plants grown,and their relations[J].PLoS One,2013,8(8):e73 013.
金刚, 王华,张昂,等.中国葡萄酒产区酒酒球菌种质资源遗传多样性分析[J].食品科学,2015,36(17):134-139.JIN G,WANG H,ZHANG A,et al.Genetic diversity analysis of Oenococcus oeni strains isolated from Chinese wines[J].Food Science,2015,36(17):134-139.
屈慧鸽.葡萄酒生产过程中醋酸菌的危害及影响因素分析[J].酿酒科技,2009(2):43-46.QU H G.Analysis of the hazard and the influencing factors of acetic acid bacteria in the production of grape wine[J].Liquor-Making Science & Technology,2009(2):43-46.
BOKULICH N A,JOSEPH C M L,ALLEN G,et al.Next-generation sequencing reveals significant bacterial diversity of botrytized wine[J].PLoS One,2012,7(5):e36 357.
GARMENDIA G,VERO S.Occurrence and biodiversity of Aspergillus section Nigri on ‘Tannat' grapes in Uruguay[J].International Journal of Food Microbiology,2016,216:31-39.
HONG Y A,PARK H D.Role of non-Saccharomyces yeasts in Korean wines produced from Campbell early grapes:Potential use of Hanseniaspora uvarum as a starter culture[J].Food Microbiology,2013,34(1):207-214.