Abstract: To investigate the heterologous expression in Bacillus licheniformis and improve the fermentation level of maltotetraose-forming amylase, a xylose-inducible expression system was constructed in B. licheniformis CICIM B1391 to express the maltotetraose-forming amylase from Pseudomonas saccharophila, and a preliminary fermentation optimization was conducted. It was observed that maltotetraose-forming amylase was successfully secreted into the fermentation broth induced by xylose. After optimized induction time, temperature and fermentation time, the maximum enzyme activity reached to (168.2±9.41) U/mL in shake flask fermentation. The optimal fermentation conditions were adding the inducer at 24 h, the induction temperature of 30 ℃, and ending fermentation at the end of stable period.
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