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食品与发酵工业  2021, Vol. 47 Issue (5): 22-27    DOI: 10.13995/j.cnki.11-1802/ts.025490
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
鲊肉粉中乳酸菌和葡萄球菌的筛选及鉴定
王曼, 杨琛, 覃晓玉, 康孟杰, 郝桂芳, 王承明*
(华中农业大学 食品科学技术学院, 湖北 武汉, 430070)
Screening and identification of lactic acid bacteria and Staphylococcus in fermented meat rice
WANG Man, YANG Chen, QIN Xiaoyu, KANG Mengjie, HAO Guifang, WANG Chengming*
(College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
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摘要 从传统发酵鲊肉粉中分离、筛选发酵性能优良的乳酸菌和葡萄球菌, 为鲊肉粉接种发酵提供理论依据。按照肉制品发酵菌株的筛选标准, 利用形态学特征和16S rDNA序列分析鉴定菌株, 筛选出2株乳酸菌A1、C7和2株葡萄球菌S6、S7。结果表明:乳酸菌菌株A1、C7均为植物乳杆菌(Lactobacillus plantarum), 能快速产酸, 具有较好抑菌特性;葡萄球菌菌株S6为沃氏葡萄球菌(Staphylococcus warneri), S7为巴氏葡萄球菌(Staphylococcus pasteuri), 能产蛋白酶和脂肪酶, 具有良好的耐盐性和耐亚硝酸盐性, 对不同抗生素有不同的敏感性。筛选得到的4株菌株具有良好的发酵特性, 乳酸菌和葡萄球菌之间无拮抗作用, 复配后用可于鲊肉粉接种发酵。
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王曼
杨琛
覃晓玉
康孟杰
郝桂芳
王承明
关键词:  鲊肉粉  葡萄球菌  乳酸菌  发酵特性  分离筛选    
Abstract: This study aimed to isolate and screen lactic acid bacteria and Staphylococcus with good fermentation property from traditionally fermented meat rice and provide theoretical basis for inoculating the fermentation of meat rice. According to the screening criteria of fermented meat products, the strains were identified through morphological characteristics and 16S rDNA sequence analysis. Finally, two strains of lactic acid bacteria (A1, C7) and two strains of Staphylococcus (S6, S7) were obtained. Both the A1 and C7 strains were Lactobacillus plantarum, which can produce acid rapidly and has antibacterial properties. The strains of S6 and S7 were Staphylococcus warneri and Staphylococcus pasteuri, respectively. The selected strains can produce protease and lipase and have good salt and nitrite resistance property, and different sensitivities to different antibiotics. The four strains screened in this study had good fermented properties, and there was no conflict between the lactic acid bacteria and Staphylococcus, thus can be used as excellent strains for the inoculation of fermented meat rice.
Key words:  fermented meat rice    Staphylococcus    lactic acid bacteria    fermentation characteristics    isolation screening
收稿日期:  2020-08-26      修回日期:  2020-09-09                发布日期:  2021-03-31      期的出版日期:  2021-03-15
基金资助: 中央高校基础研究项目(2662018PY060)
作者简介:  硕士研究生(王承明教授为通讯作者, E-mail:cmwang@mail.hzau.edu.cn)
引用本文:    
王曼,杨琛,覃晓玉,等. 鲊肉粉中乳酸菌和葡萄球菌的筛选及鉴定[J]. 食品与发酵工业, 2021, 47(5): 22-27.
WANG Man,YANG Chen,QIN Xiaoyu,et al. Screening and identification of lactic acid bacteria and Staphylococcus in fermented meat rice[J]. Food and Fermentation Industries, 2021, 47(5): 22-27.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025490  或          http://sf1970.cnif.cn/CN/Y2021/V47/I5/22
[1] 张瑶, 白娟, 王承明.鲊肉粉的加工工艺研究[J].中国酿造, 2017, 36(10):175-180.
ZHANG Y, BAI J, WANG C M.Processing technology of fermented meat rice[J].China Brewing, 2017, 36(10):175-180.
[2] 张瑶, 宫克, 汪思佚, 等.鲊肉粉发酵过程中理化指标动态变化[J].食品工业科技, 2018, 39(20):6-11;17.
ZHANG Y, GONG K, WANG S Y, et al.Dynamic changes of physicochemical indexs in fermented meat rice during fermentation[J].Science & Technology of Food Industry, 2018, 39(20):6-11;17.
[3] BUCKENHUSKES H J.Selection criteria for lactic acid bacteria to be used as starter cultures for various food commodities[J].FEMS Microbiology Reviews, 1993, 12(1-3):253-272.
[4] 张晓东.发酵香肠菌种的筛选及对香肠理化性质的影响[D].长沙:湖南农业大学, 2017.
ZHANG X D.Screening of bacteria in fermented sausage and its effects on physical and chemical properties of fermented sausage[D].Changsha:Hunan Agricultural University, 2017.
[5] LUCKE F K.Utilization of microbes to process and preserve meat[J].Meat Science, 2000, 56(2):105-115.
[6] 王俊钢, 刘成江, 李宇辉, 等.发酵肉制品中风味物质研究进展[J].江苏农业科学, 2014, 42(9):251-253;281.
WANG J G, LIU C J, LI Y H, et al.Studies on flavor of fermented meat products[J].Jiangsu Agricultural Sciences, 2014, 42(9):251-253;281.
[7] ANIHOUVI V B, SAKYI-DAWSON E, AYERNOR G S, et al.Microbiological changes in naturally fermented cassava fish (Pseudotolithus sp.) for lanhouin production[J].International Journal of Food Microbiol, 2007, 116(2):287-291.
[8] ROSEIRO L C, SANTOS C, SOL M, et al.Proteolysis in Painho de Portalegre dry fermented sausage in relation to ripening time and salt content[J].Meat Science, 2007, 79(4):784-794.
[9] ZENG X F, XIA W S, JIANG Q X, et al.Chemical and microbial properties of Chinese traditional low-salt fermented whole fish product Suan yu[J].Food Control, 2013, 30(2):590-595.
[10] FLORES M, TOLDRA F.Microbial enzymatic activities for improved fermented meats[J].Trends in Food Science & Technology, 2010, 22(2):81-90.
[11] 潘晓倩, 成晓瑜, 张顺亮, 等.不同发酵剂对北方风干香肠色泽和风味品质的改良作用[J].食品科学, 2015, 36(14):81-86.
PAN X Q, CHENG X Y, ZHANG S L, et al.Analysis of color and flavor improvement of different starter cultures on northern air-dried sausage[J].Food Science, 2015, 36(14):81-86.
[12] 林胜利.乳酸菌的筛选鉴定及其在淡水鱼糜发酵中的应用研究[D].杭州:浙江工业大学, 2012.
LIN S L.Screening of lactic bacteria and its application in the fermentation of freshwater fish surimi[D].Hangzhou:Zhejiang University of Technology, 2012.
[13] 朱明明.巴氏球菌TS-82产类胡萝卜素裂解酶及其催化类胡萝卜素降解机理的研究[D].杨凌:西北农林科技大学, 2015.
ZHU M M.Carotenoid cleavage enzyme from Staphylococcus pasteuri TS-82 and its catalytic mechanism on carotenoids degradation[D].Yangling:Northwest A&F University, 2015.
[14] 王海燕, 马长伟, 李平兰.传统湖南腊肉中产香葡萄球菌的筛选及鉴定[J].食品与发酵工业, 2006, 32(5):45-49.
WANG H Y, MA C W, LI P L.Selection and identification of aroma producing Staphylococci from Hunan traditional bacon[J].Food and Fermentation Industries, 2006, 32(5):45-49.
[15] 赵俊仁, 孔保华.自然发酵风干肠中乳酸菌的分离与鉴定[J].食品工业科技, 2010, 31(11):158-160;324.
ZHAO J R, KONG B H.Isolation and identification of lactic acid bacteria from the Chinese-style naturally dry fermented sausages[J].Science and Technology of Food Industry, 2010, 31(11):158-160;324.
[16] 潘晓倩, 赵燕, 张顺亮, 等.中温乳化香肠中一株优势腐败菌的分离鉴定与生物学特性[J].食品科学, 2016, 37(7):93-98.
PAN X Q, ZHAO Y, ZHANG S L, et al.Isolation, identification and biological characterization of a dominant spoilage strain in emulsified sausages sterilized at medium temperature[J].Food Science, 2016, 37(7):93-98.
[17] 李建, 李丽, 彭翠珍, 等.几株适用于发酵肉制品的乳酸菌的分离筛选及鉴定[J].食品研究与开发, 2018, 39(6):172-177.
LI J, LI L, PENG C Z, et al.Selection and identification of several lactic acid bacteria strains for fermented meat products[J].Food Research and Development, 2018, 39(6):172-177.
[18] 邓展瑞, 贠建民, 郭娟, 等.陇西腊肉加工过程中优势乳酸菌的分离及其发酵性能研究[J].生物技术进展, 2019, 9(2):200-209.
DENG Z R, YUN J M, GUO J, et al.Study on isolation and fermentation performance of dominant lactic acid bacteria during the processing of Longxi bacon[J].Current Biotechnology, 2019, 9(2):200-209.
[19] 刘丽莉, 杨协力.发酵肉制品中乳酸菌菌种筛选研究[J].农业机械学报, 2006, 37(8):229-231.
LIU L L, YANG X L.Screening and study of lactic acid bacteria in fermented meat products[J].Transactions of the Chinese Society for Agricultural Machinery, 2006, 37(8):229-231.
[20] 胡楠, 雷鸣, 王远一飞, 等.传统发酵蓝莓饮料中乳酸菌的分离鉴定及筛选[J].食品研究与开发, 2019, 40(23):205-211.
HU N, LEI M, WANG Y Y F, et al.Isolation, identification and screen of lactic acid bacteria from traditional fermented blueberry beverages[J].Food Research and Development, 2019, 40(23):205-211.
[21] 吉莉莉, 魏艳, 何丹, 等.发酵香肠中分离纯化的三株乳酸菌产酸特性研究[J].中国调味品, 2020, 45(2):36-39.
JI L L, WEI Y, HE D, et al.Study on acid production characteristics of three strains of lactic acid bacteria isolated and purified from fermented sausage[J].China Condiment, 2020, 45(2):36-39.
[22] 刘夏炜.内蒙古传统肉制品中葡萄球菌的分离筛选及其在羊肉发酵香肠中的应用[D].呼和浩特:内蒙古农业大学, 2015.
LIU X W.The separation of Staphylococcus in Inner Mongolia traditional meat products and the application of fermented mutton sausage[D].Hohhot:Inner Mongolia Agricultural University, 2015.
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