Abstract: The α-L-rhamnosidase plays an important role in the conversion of natural products, and Pichia pastoris was used to express α-L-rhamnosidase AnRhaE. The pPIC9K-AnRhaE expression vector was constructed and transformed into Pichia pastoris GS115 and KM71H. After induced expression, SDS-PAGE electrophoresis showed that GS115 was more conducive to the production of AnRhaE. High AnRhaE activity was obtained at 45-55 ℃, and the optimum temperature was 55 ℃. AnRhaE was stable at pH 4.5-8, and the optimum pH was 5. AnRhaE could hydrolyze α-1,2, α-1,6 and rhamnosyl-rhamnosyl glycosidic bond, including naringin dihydrogen chalcone to trilobatin, naringin to prunin, rutin to isoquercetin, neohesperidin to hesperetin-7-O-glucoside, hesperidin to hesperetin-7-O-glucoside and epimedin C to icariin. AnRhaE had high activity to α-1,2 glycosidic bond than the α-1,6 glycosidic bond, and could complete hydrolysis epimedin C to icariin. Therefore, AnRhaE is potentially applicable in enzymatic conversion of natural products.
卢宇靖,林育成,叶德晓,等. α-L-鼠李糖苷酶AnRhaE在毕赤酵母中的表达及应用[J]. 食品与发酵工业, 2021, 47(3): 25-30.
LU Yujing,LIN Yucheng,YE Dexiao,et al. Expression of α-L-rhamnosidase AnRhaE in Pichia pastoris and its application[J]. Food and Fermentation Industries, 2021, 47(3): 25-30.
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2021, Vol. 47 
